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The Difference in Low-Temperature Tolerance of Arabidopsis thaliana Plants and Its Ethylene-Insensitive Mutants Is Related to Activities of Antioxidant Enzymes

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Abstract

Arabidopsis thaliana Heynh. (L.) plants of the Columbia ecotype (Col-0) and its ethylene-insensitive etr1-1 (ethylene resistant 1) and ein2-1 (ethylene insensitive 2) mutants were studied. The plants were compared in respect to their cold tolerance conferred by cold acclimation. The tolerance to negative temperature increased in all the three genotypes after 5-day cooling of the plants at 2°C. Meanwhile, a quantitative difference was observed between them: the Col-0 plants survived better than the mutants, and the electrolyte leakage from the tissues reached 50% at higher (by 1°C and more) temperature in the hardened mutants than in the Col-0. Only the mutants manifested the increased lipid peroxidation over the hardening period; this indicates ROS production and general oxidative stress. From this point, we speculated that the tested Arabidopsis genotypes are different in the efficiency of their antioxidant systems. For testing, the total activities of the chief antioxidant enzymes superoxide dismutase (SOD) and catalase, including their isoenzymes, were monitored in the course of hardening. The total SOD activity was found to be lower in the mutants than in the Col-0 both at the normal growing temperature and over the whole time of the cold hardening. The isozyme analysis revealed the link of the reduced total SOD activity of the mutants with the reduced activity of their Cu/Zn-SOD isoforms at 22°C and at the beginning of the hardening. This relationship was supported by the lower relative content of the CSD1 and CSD2 gene transcripts of Cu/Zn-SOD in the mutants in comparison with the Col-0. The decrease in the total catalase activity was also observed and would be ascribed to the lowered activity of the CAT2 isoform. The reported results evidence to the principal feasibility of the ethylene signaling control of the Cu/Zn-SOD and catalase activities associated with the cold acclimation of Arabidopsis.

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Funding

This work was supported by the Ministry of Education and Science of the Russian Federation and was carried out through State Task no. AAAA-A19-119080690056-3.

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276, Moscow, Russia

    M. S. Sin’kevich, N. V. Naraikina, G. P. Alieva, N. V. Astakhova, T. I. Trunova & I. E. Moshkov

Authors
  1. M. S. Sin’kevich
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  2. N. V. Naraikina
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  3. G. P. Alieva
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  4. N. V. Astakhova
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  5. T. I. Trunova
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  6. I. E. Moshkov
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Corresponding author

Correspondence to N. V. Naraikina.

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The authors declare that they have no conflict of interest. This article does not contain any work conducted on animal or human participants.

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Translated by A. Aver’yanov

Abbreviations: CAT1, CAT2, CAT3—isoforms of catalase; CBF—C-repeat binding factor; ein2-1—ethylene insensitive 2 mutant; ERF—ethylene response factor; etr1-1—ethylene resistant 1 mutant; NBT—nitroblue tetrazolium; SOD—superoxide dismutase.

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Sin’kevich, M.S., Naraikina, N.V., Alieva, G.P. et al. The Difference in Low-Temperature Tolerance of Arabidopsis thaliana Plants and Its Ethylene-Insensitive Mutants Is Related to Activities of Antioxidant Enzymes. Russ J Plant Physiol 67, 1083–1093 (2020). https://doi.org/10.1134/S1021443720050155

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