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Shift in Expression of the Genes of Primary Metabolism and Chloroplast Transporters in Chlamydomonas reinhardtii under Different Trophic Conditions

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Abstract

Chlamydomonas reinhardtii P.A. Dangeard is a unicellular green alga capable to assimilate acetate. C. reinhardtii growth and metabolism distinctly depend on trophic conditions. Its influence on batch culture interferes with changes in the medium composition and metabolism of microalgae occurring during culture growth. The aim of this work was to estimate the effect of acetate on changes in the expression of 32 genes encoding enzymes of central metabolism and plastid transporters during growth of batch cultures of C. reinhardtii cc-124. In autotrophic conditions, transcription profiles considerably differed from the profiles in the presence of acetate. The strongest influence of trophic conditions was observed in the log phase of growth. In the presence of acetate, a more intense expression of gene ACS2 encoding plastid acetyl-CoA synthase and of the genes encoding subunits of enzymes directing acetyl groups to the synthesis of fatty acids was recorded. Elevated expression of genes PCK1 and PPT1 under mixotrophic conditions may be corresponded to the entry of acetate carbon to gluconeogenesis. In the presence of acetate, a high expression level of the starch metabolism genes was observed. Autotrophic conditions were notable for an elevated accumulation of transcripts of the genes encoding subunits of citrate lyase, which may be responsible for an outflow of acetyl groups from the Krebs cycle. Moreover, a higher level of expression was shown for genes of plastid transporters participating in the export of sugars from plastids, which is associated with the redistribution of reducing power in the cell. It was concluded that, on the level of transcription, similarity between mixotrophic and autotrophic cultures becomes more pronounced in the course of development.

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Funding

This work was partially supported by the Russian Foundation for Basic Research, project no. 19-04-00655, and by a State Assignment given to Komarov Botanical Institute, Russian Academy of Sciences, no. АААА-А18-118032390136-5.

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Authors and Affiliations

  1. St. Petersburg State University, Department of Plant Physiology and Biochemistry, 199034, St. Petersburg, Russia

    R. K. Puzanskiy & M. F. Shishova

  2. Komarov Botanical Institute of the Russian Academy of Sciences, Laboratory of Dynamics of the Arctic Vegetation Cover, 197376, St. Petersburg, Russia

    R. K. Puzanskiy

  3. All-Russia Research Institute of Agricultural Microbiology, Laboratory of Genetics of Plant-Microbial Interactions, 196608, St. Petersburg, Russia

    D. A. Romanyuk

Authors
  1. R. K. Puzanskiy
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  2. D. A. Romanyuk
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  3. M. F. Shishova
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Correspondence to M. F. Shishova.

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This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by N. Balakshina

Abbreviations: DAI—day after inoculation; FDR—false discovery rate; MWW—Mann–Whitney–Wilcoxon test; (O)PLS-DA—(orthogonal) partial least squares (or projection to latent structures) discriminant analysis; PC—principal component; PCA—principal component analysis; TAP—Tris acetate phosphate medium; TM—Tris minimal medium; VIP—variable importance in projection.

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Puzanskiy, R.K., Romanyuk, D.A. & Shishova, M.F. Shift in Expression of the Genes of Primary Metabolism and Chloroplast Transporters in Chlamydomonas reinhardtii under Different Trophic Conditions. Russ J Plant Physiol 67, 867–878 (2020). https://doi.org/10.1134/S102144372005012X

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