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iTRAQ-Based Proteomic Reveals Cell Cycle and Translation Regulation Involving in Peanut Buds Cold Stress

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Abstract

Cold stress is one major threats to field crops. The cold tolerant ability is a key limiting factor for the popularization of peanut (Arachis hypogaea L.) in cold areas. Our previous research showed three key metabolic change time points of peanut buds in response to low temperature. The present study applied iTRAQ proteomic to further quantify differentially expressed proteins in three key metabolic change time points. A total of 2684 non-redundant proteins and 333 differentially expressed proteins across the three cold stress treatment points were identified. The results enrich the cold stress regulation network and provide useful information for further improving the ability of peanut under cold stress.

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Funding

This research was supported by Basal Research Fund of Jiangsu Academy of Agricultural Sciences ZX(18)2014, Xuzhou Science and Technology Project KC16NG068, and Liaoning S&T Project (2014024, 2014201002).

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    Authors and Affiliations

    1. Xuzhou Academy of Agricultural Sciences, 221131, Xuzhou, Jiangsu, PR China

      X. J. Wang, D. L. Sun, N. F. Bian, Z. M. Zhang & X. Wang

    2. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, PR China

      Y. Shen, Z. D. Chen, Y. H. Liu & Y. Shen

    3. Institute of Sandy Land Amelioration and Utilization of Liaoning, 123000, Liaoning, PR China

      P. X. Shi

    Authors
    1. X. J. Wang
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    2. Y. Shen
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    3. D. L. Sun
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    4. N. F. Bian
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    5. P. X. Shi
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    6. Z. M. Zhang
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    Contributions

    X.W. and Y.S. contributed equally to this work. X.W. and Y.S. performed the experiments. D.S., N.B. and P.S analysed the data. Z.Z., Z.C. and Y.L. helped with experiments. Y.S. and X.W. provided overall supervision, supervised the experiments and wrote the manuscript.

    Corresponding authors

    Correspondence to Y. Shen or X. Wang.

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    This article does not contain any studies using animals as objects or any research involving people as objects of research. The authors declare that they have no conflict of interest.

    Additional information

    Abbreviations: iTRAQ—isobaric tags for relative and absolute quantitation; 2D—two-dimensional; 2D DIGE—2D difference gel electrophresis.

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    Wang, X.J., Shen, Y., Sun, D.L. et al. iTRAQ-Based Proteomic Reveals Cell Cycle and Translation Regulation Involving in Peanut Buds Cold Stress. Russ J Plant Physiol 67, 103–110 (2020). https://doi.org/10.1134/S1021443720010239

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    • DOI: https://doi.org/10.1134/S1021443720010239

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