Skip to main content
Log in

Lipid Composition, Content of Polyphenols, and Antiradical Activity in Some Representatives of Marine Algae

Russian Journal of Plant Physiology Aims and scope Submit manuscript


Qualitative and quantitative composition of the lipid complex and total content of polyphenols were compared in aqueous alcoholic extracts from three species of marine macrophytes collected in Peter the Great Bay, the Sea of Japan. Examined seaweeds belonged to different phyla: green alga Ulva lactuca L. sea lettuce, brown alga Sargassum pallidum (Turner) C. Agardh sargassum pallidum, and red alga Ahnfeltia tobuchiensis (Kanno et Matsubara) Makijenko ahnfeltia tobuchiensis. It was shown that glycolipids (30.3–41.5%) and neutral lipids (34–48.5%) prevailed among total lipids in the extracts from all three algal species; phospholipids accounted for 10–25.7% of total lipids. Among neutral lipids in all the seaweeds, triacylglycerols and free sterols prevailed. The greatest quantity of triacylglycerols was found in A.tobuchiensis and that of free sterols was in S. pallidum. As to the individual fractions of phospholipids, their composition and content considerably differed. In terms of content, predominant phospholipid fractions in the extract from U. lactuca were phosphatidyl glycerol, phosphatidyl ethanolamine, and phosphatidyl inositol, those from S. pallidum were phosphatidyl ethanolamine and phosphatidyl glycerol, and those from A. tobuchiensis were phosphatidyl choline and phosphatidyl glycerol. As to the percentage of main fatty acids comprising the lipid component of the extracts from the examined algal species, it was found that the extract from brown alga S. pallidum contained the greatest content of PUFA from the family n-6. The extract from green alga U. lactuca contained the greatest content of PUFA from the family n-3. In the extract from red alga A. tobuchiensis, arachidonic (family n-6) and eicosapentaenoic (family n-3) acids prevailed among PUFA. The greatest quantity of polyphenols was detected in the extract from brown alga S. pallidum, which 15–24 times exceeded respective figures in U. lactuca and A. tobuchiensis. Moreover, the extract from S. pallidum showed a much higher level of antiradical activity in respect to ABTS+, which was 5–12.5 times greater than in U. lactuca and A. tobuchiensis, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions


  1. Michalak, I. and Chojnacka, K., Algae as production systems of bioactive compounds—a review, Eng. Life Sci., 2015, vol. 15, pp. 160–176.

    Article  CAS  Google Scholar 

  2. Khotimchenko, S.V., Lipids of Marine Macrophytic A-lgae and Grasses: Structure, Distribution, Analysis, Vladivostok: Dalnauka, 2003.

    Google Scholar 

  3. Goncharova, S.N., Kostetsky, E.Y., and Sanina, N.M., The effect of seasonal shifts in temperature on the lipid composition of marine macrophytes, Russ. J. Plant Physiol., 2004, vol. 51, pp. 169–175.

    Article  CAS  Google Scholar 

  4. Schoenwaelder, M.E.A. and Clayton, M.N., The presence of phenolic compounds in isolated cell walls of brown algae, Phycologia, 1999, vol. 38, pp. 161–166.

    Article  Google Scholar 

  5. Ragan, M.A. and Glombitza, K.W., Phlorotannins, brown algal polyphenols, Prog. Phycol. Res., 1986, vol. 4, pp. 129–241.

    CAS  Google Scholar 

  6. Fomenko, S.E., Kushnerova, N.F., and Lesnikova, L.N., Experimental assessment of the efficiency of erythrocyte membrane repair by an extract of the tunic of the ascidian purple sea squirit in carbon tetrachloride poisoning, Pharm. Chem. J., 2013, vol. 46, no. 10, pp. 606–611.

    Article  CAS  Google Scholar 

  7. Sprygin, V.G., Fomenko, S.E., Kushnerova, N.F., Sizova, L.A., and Momot, T.V., The hepatoprotective properties of an extract from the brown alga Saccharina japonica,Russ. J. Mar. Biol., 2013, vol. 39, pp. 65–69.

    Article  Google Scholar 

  8. Fomenko, S.E., Kushnerova, N.F., Sprygin, V.G., and Momot, T.V., The antioxidant and stress-protective properties of an extract from the green alga Ulva lactuca Linnaeus, 1753, Russ. J. Mar. Biol., 2013, vol. 42, pp. 509–514.

    Article  Google Scholar 

  9. Hwang, E.S. and Thi, N.D., Effects of extraction and processing methods on antioxidant compound contents and radical scavenging activities of laver (Porphyra te-nera), Prev. Nutr. Food Sci., 2014, vol. 19, pp. 40–48.

    Article  Google Scholar 

  10. Folch, J., Less, M., and Sloane-Stanley, G.H., A simple method for the isolation and purification of total lipids from animal tissue, Biol. Chem., 1957, vol. 226, pp. 497–509.

    CAS  Google Scholar 

  11. Svetachev V.I. and Vaskovsky, V., A simplified technique for thin-layer microchromatography of lipids, J. Chromatogr., 1972, vol. 67, pp. 376–378.

    Article  Google Scholar 

  12. Vaskovsky, V.E. and Khotimchenko, S.V., HPTLC of polar lipids of algae and other plants, Sep. Sci., 1982, vol. 5, pp. 635–636.

    CAS  Google Scholar 

  13. Vaskovsky, V.E., Kostetsky, E.Y., and Vasendin, I.M., Universal reagent for phospholipid analysis, J. Chromatogr., 1975, vol. 114, pp. 129–141.

    Article  CAS  Google Scholar 

  14. Van Gent, C.M., Roseleur, O.J., and van der Bijl, P., Detection of cerebrosides on thin-layer chromatograms with an anthrone spray reagent, J. Chromatogr., 1973, vol. 85, pp. 174–176.

    Article  CAS  Google Scholar 

  15. Rouser, G., Kritchevsky, G., and Yamamoto, A., Column chromatographic and associated procedures, in Lipid Chromatographic Analysis, Marinetti, G.V., Ed., New York: Dekker, 1967, vol. 1, pp. 99–162.

    Google Scholar 

  16. Kates, M., Techniques of Lipidology: Isolation, Analysis and Identification of Lipids, New York: Elsevier, 1972.

    Book  Google Scholar 

  17. Wagner, H., Horhammer, L., and Wolf, F., Thin-layer chromatography of phosphatides and glycolipides, Bioche-m. Z., 1961, vol. 334, pp. 175–184.

    CAS  Google Scholar 

  18. Amenta, J.S., A rapid chemical method for quantification of lipids separated by thin-layer chromatography, J. Lipid Res., 1964, vol. 5, pp. 270–272.

    CAS  PubMed  Google Scholar 

  19. Carreau, J.P. and Dubaco, J.P., Adaptation of macro-scale method to the micro-scale for fatty acid methyl transesterification of biological lipid extracts, J. Chromatogr., 1978, vol. 151, pp. 384–390.

    Article  CAS  Google Scholar 

  20. Christie, W.W., Equivalent chain-lengths of methyl ester derivatives of fatty acids on gas chromatography: a reappraisal, J. Chromatogr., 1988, vol. 447, pp. 305–314.

    Article  CAS  Google Scholar 

  21. Parys, S., Rosenbaum, A., Kehraus, S., Reher, G., Glombitza, K.W., and König, G.M., Evaluation of quantitative methods for the determination of polyphenols in algal extracts, J. Nat. Prod., 2007, vol. 70, pp. 1865–1870.

    Article  CAS  Google Scholar 

  22. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C., Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med., 1999, vol. 26, pp. 1231–1237.

    Article  CAS  Google Scholar 

  23. Bartosz, G., Janaszewska, A., Ertel, D., and Bartosz, M., Simple determination of peroxyl radical-trapping capacity, Biochem. Mol. Biol., 1998, vol. 46, pp. 519–528.

    CAS  Google Scholar 

  24. Sanina, N.M., Goncharova, S.N., and Kostetsky, E.Y., Seasonal changes of fatty acid composition and thermotropic behavior of polar lipids from marine macrophytes, Phytochemistry, 2008, vol. 69, pp. 1517–1527.

    Article  CAS  Google Scholar 

  25. Dembitsky, V.M. and Rozentsvet, O.A., Diacylglyceryltrimethylhomoserines and phospholipids of some green marine macrophytes, Phytochemistry, 1989, vol. 28, pp. 3341–3343.

    Article  Google Scholar 

  26. Parys, S., Kehraus, S., and Pete, R., Seasonal variation of polyphenolics in Ascophyllum nodosum (Pheophyceae), Eur. J. Phycol., 2009, vol. 44, pp. 331–338.

    Article  CAS  Google Scholar 

  27. Tierney, M.S., Soler-Vila, A., Rai, D.K., Croft, A.K., Brunton, N., and Smyth, T.J., UPLC-MS profiling of low molecular weight phlorotannin polymers in As-cophyllum nodosum, Pelvetia canaliculata and Fucus spiralis,Metabolomics, 2014, vol. 10, pp. 534–535.

    Article  Google Scholar 

  28. Alagan, V.T., Valsala, R.N., and Rajesh, K.D., Bioactive chemical constituent analysis, in vitro antioxidant and antimicrobial activity of whole plant methanol extracts of Ulva lactuca Linn., Br. J. Pharm. Res., 2017, vol. 15, pp. 1–14.

    Article  CAS  Google Scholar 

  29. De Quiros, A.R.B., Lage-Yusty, M.A., and Lopez-Hernandez, J., Determination of phenolic compounds in macroalgae for human consumption, Food Chem., 2010, vol. 121, pp. 634–638.

    Article  Google Scholar 

  30. Shibata, T., Ishimaru, K., Kawaguchi, S., Yoshikawa, H., and Hama, Y., Antioxidant activities of phlorotannins isolated from Japanese Laminariaceae, J. Appl. Phycol., 2008, vol. 20, pp. 705–711.

    Article  CAS  Google Scholar 

Download references


We thank the reviewers and scientific editor for critical remarks and insertions that improved the paper.


This work was supported by budgetary funds.

Author information

Authors and Affiliations


Corresponding author

Correspondence to S. E. Fomenko.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by N. Balakshina

Abbreviations: ARA—antiradical activity; DGTS—1,2-diacylglyceryl-О-4-(N,N,N-trimethyl)-homoserine; GA—gallic acid; MUFA—monounsaturated fatty acids; NL—neutral lipids; PC—phosphatidyl choline; PE—phosphatidyl ethanolamine; PG—phosphatidyl glycerol; PHEG—phosphatidyl-О-[N-(2-hydroxyethyl) glycine]; PI—phosphatidyl inositol; PL—phospholipids; PP—polyphenols; PS—phosphatidyl serine; PUFA—polyunsaturated fatty acids; SFA—saturated fatty acids; TAG—triacylglycerols; TPP—total polyphenols.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fomenko, S.E., Kushnerova, N.F., Sprygin, V.G. et al. Lipid Composition, Content of Polyphenols, and Antiradical Activity in Some Representatives of Marine Algae. Russ J Plant Physiol 66, 942–949 (2019).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: