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Effect of Pseudomonas Bacteria on Peroxidase Activity in Wheat Plants when Infected with Bipolaris sorokiniana

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Abstract

We investigated the effect of treating soft wheat seeds (Triticum aestivum L.) with two Pseudomonas bacteria strains, isolated from earthworm coprolites, showing a significant antifungal and growth-promoting action in preliminary screening on the activity of guaiacol-dependant peroxidase under phytopathogenic load in the presence of Bipolaris sorokiniana (Sacc.) Shoemaker as a mechanism for inducing plant resistance to the pathogen. We established a statistically significant decrease (P < 0.05) in root rot disease incidence and severity during bacterization, which is indicative both of antifungal activity of the used bacterial isolates and of their successful colonizing the rhizosphere of wheat plants. We noted a response of free and weakly bound peroxidase of wheat plants to infection with B. sorokiniana: the enzyme activity increased during pathogenesis. Bacterization also increased peroxidase activity in plant leaves and roots, the greatest differences from non-bacterized plants being observed in wheat roots in the presence of the pathogen. We detected a direct link between peroxidase activity in wheat roots and leaf tissues in the absence of the pathogen and the feedback between peroxidase activity and plant infestation by the root rot pathogen. In the presence of the phytopathogen, there is a lack of correlation between peroxidase activity in wheat roots and leaves, and there is a shift of activity towards its increase in roots, which plays an important role in the development of systemic resistance against the root rot pathogen that penetrates into plants through the roots and root collar.

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Authors and Affiliations

  1. Institute of Biology, Tomsk State University, Tomsk, 634050, Russia

    O. M. Minaeva, E. E. Akimova, N. N. Tereshchenko, T. I. Zyubanova & M. V. Apenysheva

  2. BIOCENTR, Siberian Research Institute of Agriculture and Peat, Branch of the Federal State Budgetary Institution of Science, Siberian Federal Scientific Center of Agrobiotechnologies, Russian Academy of Sciences, Tomsk, 634050, Russia

    E. E. Akimova, N. N. Tereshchenko, T. I. Zyubanova & A. V. Kravets

Authors
  1. O. M. Minaeva
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  2. E. E. Akimova
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  3. N. N. Tereshchenko
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  4. T. I. Zyubanova
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  5. M. V. Apenysheva
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  6. A. V. Kravets
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Correspondence to O. M. Minaeva.

Additional information

Published in Russian in Fiziologiya Rastenii, 2018, Vol. 65, No. 5, pp. 366–375.

The article was translated by authors.

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Minaeva, O.M., Akimova, E.E., Tereshchenko, N.N. et al. Effect of Pseudomonas Bacteria on Peroxidase Activity in Wheat Plants when Infected with Bipolaris sorokiniana. Russ J Plant Physiol 65, 717–725 (2018). https://doi.org/10.1134/S1021443718040052

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  • DOI: https://doi.org/10.1134/S1021443718040052

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