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Response of Сucumber (Cucumis sativus L.) Plants to Prolonged Permanent and Short-Term Daily Exposures to Chilling Temperature

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Abstract

We investigated the response of chilling-sensitive plants of cucumber (Cucumis sativus L.) to prolonged permanent (6 days) (PLT) and short-term (3 h) daily exposures to low non-freezing temperatures (9 and 12°С) (DROP) lying on different sides of the critical value (10°С) corresponding to a phase transition of membrane lipids in chilling-sensitive plants from a liquid-crystalline to a solid gel structure. Effects and aftereffects of DROP treatments at temperatures of 9 and 12°С (DROP9 and DROP12, respectively) were identical. They caused a reduction in linear dimensions of plants (as compared with control plants) and enhanced chilling tolerance of leaves but did not influence photosynthetic activity and water relations. However, when exposure to these temperatures was permanent (PLT9 and PLT12), their effects on plants were different. PLT9 almost entirely suppressed plant growth and development, inactivated photosynthetic apparatus (PSA), increased relative electrolite leakage (REL), and boosted lipid peroxidation (LPO). PLT12 also considerably reduced plant height and leaf area and suppressed (but did not inactivate) PSA; it did not boost POL or increased REL. It is important that, after the termination of chilling, PLT9 plants (unlike PLT12 plants) could not quickly resume growth and restore photosynthetic activity. Thus, considerable differences in plant responses to various patterns of chilling (long permanent or short-term daily) are probably due to the fact that, in the case of DROP treatments, relatively short-term (3 h) chilling of plants is followed in the diurnal cycle by a prolonged period (21 h) of optimal temperature when possible deviations and/or disturbances of PSA are restored and toxic substances that could accumulate in the course of chilling metabolized and/or neutralized. Pronounced differences in plant response to permanent exposure to temperatures of 9 and 12°С probably depend on the fact that these temperatures lie on different sides of a critical value (10°С) below which chilling-sensitive plants suffer grave physiological disturbances due to cooling. In addition, we showed that different responses of plants to PLT and DROP treatments are not determined by a usual dose-effect relationship but depend in many respects on the pattern of temperature influence (prolonged or short-term, single or recurring). As a result, the number of DROP spans experienced by plants in the experiments proved to be more important than their duration (at least, within a time interval from 2 to 12 h).

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Abbreviations

CT:

chilling tolerance

DROP:

short-term daily drops of temperature

MDA:

malonic dialdehyde

PAR:

photosynthetically active radiation

PLT:

permanent low temperature

LPO:

lipid peroxidation

PS:

photosystem

PSA:

photosynthetic apparatus

REL:

relative electrolyte leakage

RWC:

relative water content

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Institute of Biology, Karelian Research Center, Russian Academy of Sciences, Petrozavodsk, 185910, Russia

    T. G. Shibaeva, E. G. Sherudilo & A. F. Titov

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  1. T. G. Shibaeva
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  2. E. G. Sherudilo
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  3. A. F. Titov
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Correspondence to T. G. Shibaeva.

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Original Russian Text © T.G. Shibaeva, E.G. Sherudilo, A.F. Titov, 2018, published in Fiziologiya Rastenii, 2018, Vol. 65, No. 2, pp. 143–152.

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Shibaeva, T.G., Sherudilo, E.G. & Titov, A.F. Response of Сucumber (Cucumis sativus L.) Plants to Prolonged Permanent and Short-Term Daily Exposures to Chilling Temperature. Russ J Plant Physiol 65, 286–294 (2018). https://doi.org/10.1134/S1021443718020061

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