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Salicylic Acid (SA) Induced Alterations in Growth, Biochemical Attributes and Antioxidant Enzyme Activity in Faba Bean (Vicia faba L.) Seedlings under NaCl Toxicity

Russian Journal of Plant Physiology volume 65, pages 104–114 (2018)Cite this article

Abstract

In the present study we tried to evaluate the effect of salicylic acid (SA) in alleviating the negative effects of salinity stress. NaCl stress (50 and 100 mM) declines the shoot and root length and maximum decrease was observed at 100 mM concentration of NaCl. Similarly shoot dry weight decreased by 57.14% and root dry weight by 67.24% with 100 mM NaCl stress. The pigments and leaf relative water content (LRWC) were also observed to decline with increase in NaCl concentration. However, supplementation of SA to NaCl stressed seedlings showed enhanced length and dry weight of shoot and root. The pigment and LRWC also increased by the application of SA in the present study. NaCl stress also enhanced proline and glycine betaine (GB) by 3.01 and 2.04 folds, respectively; further enhancement was recorded by the application of SA. Hydrogen peroxide (H2O2) and malondialdehyde (MDA) content also showed rise in accumulation, however, seedlings treated with SA and NaCl (100 mM + SA) declines the H2O2 accumulation to 1.90 from 2.45 folds and MDA to 1.69 from 2.34 folds over the control. Antioxidants were observed to increase with NaCl concentration and further increase was recorded by the application of SA. Indoleacetic acid (IAA) and indole butyric acid (IBA) decreased by 36.60 and 44.16%, respectively, and ABA increased by 750% with 100 mM NaCl. Addition of SA to NaCl stressed seedlings enhanced the IAA and IBA and decreased the ABA concentration to appreciable level. NaCl is also responsible for the higher accumulation of Na+ and Na+/K+ ratio and decreased uptake of Ca2+ and K+. Supplementation of SA decreased the Na+ accumulation and enhanced the uptake of Ca2+ and K+ in NaCl stressed seedlings. In conclusion, SA supplementation mitigates the negative effects of NaCl toxicity in faba bean seedlings through the modulation of different osmoprotectants, antioxidants and nutrients uptake.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

DAT:

days after treatment

GB:

glycine betaine

GR:

glutathione reductase

SA:

salicylic acid

LRWC:

leaf relative water content

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Authors and Affiliations

  1. Botany and Microbiology Department, College of Science, King Saud University, P. O. Box 2460, Riyadh, 11451, Saudi Arabia

    P. Ahmad, M. N. Alyemeni & L. Wijaya

  2. Department of Botany, S.P. College, Srinagar, Jammu and Kashmir, 190001, India

    P. Ahmad

  3. School of Studies in Botany Jiwaji University, Gwalior, MP-474011, India

    M. A. Ahanger

  4. Institute of Landscape Biogeochemistry, Leibniz Centre for Agricultural Landscape Research, Müncheberg, Germany

    D. Egamberdieva

  5. Biology Department, College of Science and Humanities, Prince Sattam bin Abdulaziz University (PSAU), Alkharj, 11942, Kingdom of Saudi Arabia

    P. Alam

Authors
  1. P. Ahmad
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  2. M. N. Alyemeni
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  4. D. Egamberdieva
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Correspondence to P. Ahmad.

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Ahmad, P., Alyemeni, M.N., Ahanger, M.A. et al. Salicylic Acid (SA) Induced Alterations in Growth, Biochemical Attributes and Antioxidant Enzyme Activity in Faba Bean (Vicia faba L.) Seedlings under NaCl Toxicity. Russ J Plant Physiol 65, 104–114 (2018). https://doi.org/10.1134/S1021443718010132

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  • DOI: https://doi.org/10.1134/S1021443718010132

Keywords

  • Vicia faba
  • lipid peroxidation
  • antioxidants
  • osmolytes
  • salicylic acid
  • salinity stress