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Russian Journal of Plant Physiology

, Volume 64, Issue 6, pp 883–888 | Cite as

Antioxidant capacity and cadmium accumulation in parsley seedlings exposed to cadmium stress

  • Y. Ulusu
  • L. Öztürk
  • M. Elmastaş
Research Papers
  • 118 Downloads

Abstract

Parsley (Petroselinum hortense L.) plants cultivated under controlled conditions were exposed to different doses of cadmium to investigate the antioxidant capacity and cadmium accumulation in the samples. Two-months-old parsley seedlings were treated with four different concentrations of CdCl2 (0, 75, 150, and 300 μM) for fifteen days. Cadmium level in leaves increased significantly by increasing the Cd contamination in the soil. Total chlorophyll and carotenoid content declined considerably with Cd concentration. Cd treatment caused a significant increase lipid peroxidation in tissue of leaf. Superoxide dismutase activity (SOD, EC 1.15.1.1) increased partially at 75 and 150 μM CdCl2 concentrations whereas the activity decreased at 300 μM CdCl2. Catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11) activities were reduced by Cd application. Total phenolic compound amount increased significantly with increasing Cd concentration, as ferric reduction power, superoxide anion radical, and DPPH˙ free radical scavenging activities elevated slightly by the concentration. These results suggest that antioxidant enzymes activities were repressed depending on accumulation of cadmium in leaves of parsley while the non-enzymatic antioxidant activities slightly increased.

Keywords

Petroselinum hortense ascorbate peroxidase antioxidant activity cadmium catalase photosynthetic pigment polyphenol contents superoxide dismutase 

Abbreviations

APX

ascorbate peroxidase

CAT

catalase

DPPH˙

2,2-diphenyl-1-picryl-hydrazil radical

FRAP

Fe3+ reducing antioxidant power

SOD

superoxide dismutase

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References

  1. 1.
    Michalack, A., Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress, Pol. J. Environ. Stud., 2006, vol. 15, no. 4, pp. 523–530.Google Scholar
  2. 2.
    Valko, M., Morris, H., and Cronin, M.T.D., Metals, toxicity and oxidative stress, Curr. Med. Chem., 2005, vol. 12, no. 10, pp. 1161–1208.CrossRefPubMedGoogle Scholar
  3. 3.
    Dixit, V., Pandey, V., and Shyam, R., Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad), J. Exp. Bot., 2001, vol. 52, no. 358, pp. 1101–1109.CrossRefPubMedGoogle Scholar
  4. 4.
    Groppa, M.D., Ianuzzo, M.P., Rosales, E.P., Vázquez, S.C., and Benavides, M.P., Cadmium modulates NADPH oxidase activity and expression in sunflower leaves, Biol. Plant., 2012, vol. 56, no. 1, pp. 167–171.CrossRefGoogle Scholar
  5. 5.
    Shah, K. and Nongkynrih, J.M., Metal hyperaccumulation and bioremediation, Biol. Plant., 2007, vol. 51, no. 4, pp. 618–634.CrossRefGoogle Scholar
  6. 6.
    Kovácik, J.E., Klejdus, B., Hedbavny, J., Štork, F., and Backor, M., Comparison of cadmium and copper effect on phenolic metabolism, mineral nutrients and stressrelated parameters in Matricaria chamomilla plants, Plant Soil, 2009, vol. 320, nos. 1–2, pp. 231–242.Google Scholar
  7. 7.
    Dey, S.K., Dey, J., Patra, S., and Pothal, D., Changes in the antioxidative enzyme activities and lipid peroxidation in wheat seedlings exposed to cadmium and lead stress, Braz. J. Plant Physiol., 2007, vol. 19, no. 1, pp. 53–60.CrossRefGoogle Scholar
  8. 8.
    Kisa, D., Elmastas, M., Öztürk, L., and Kayir, Ö., Responses of the phenolic compounds of Zea mays under heavy metal stress, Appl. Biol. Chem., 2016, vol. 59, no. 6, pp. 813–820.CrossRefGoogle Scholar
  9. 9.
    Jemal, F., Didierjean, L., Ghrir, R., Ghorbal, M.H., and Burkard, G., Characterization of cadmium binding peptides from pepper (Capsicum annuum), Plant Sci., 1998, vol. 137, no. 2, pp. 143–154.CrossRefGoogle Scholar
  10. 10.
    Arnon, D.I., Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris, Plant Physiol., 1949, vol. 24, no. 1, pp. 1–15.CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Jaspars, E.M.J., Pigmentation of tobacco crown-gall tissues cultured in vitro in dependence of the composition of the medium, Physiol. Plant., 1965, vol. 18, no. 4, pp. 933–940.CrossRefGoogle Scholar
  12. 12.
    Velikova, V., Yordanov, I., and Edrava, A., Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines, Plant Sci., 2000, vol. 151, no. 1, pp. 59–66.CrossRefGoogle Scholar
  13. 13.
    Beyer, W.F., Jr. and Fridovich, I., Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions, Anal. Biochem., 1987, vol. 161, no. 2, pp. 559–566.CrossRefPubMedGoogle Scholar
  14. 14.
    Havir, E.A. and Mchale, N.A., Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves, Plant Physiol., 1987, vol. 84, no. 2, pp. 450–455.CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Wang, S.Y., Jiao, J.H., and Faust, M., Changes in ascorbate, glutathione and related enzyme activities during thidiazuron-induced bud break of apple, Plant Physiol., 1991, vol. 82, no. 2, pp. 231–236.Google Scholar
  16. 16.
    Singleton, V.L., Orthofer, R., and Lamuela-Raventos, R.M., Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent, Methods Enzymol., 1999, vol. 299, pp. 152–178.CrossRefGoogle Scholar
  17. 17.
    Oyaizu, M., Studies on product of browning reaction prepared from glucose amine, Jpn. J. Nutr., 1986, vol. 44, no. 6, pp. 307–315.CrossRefGoogle Scholar
  18. 18.
    Beauchamp, C. and Fridovich, I., Superoxide dismutase: improved assays and an assay applicable to acrylamide gels, Anal. Biochem., 1971, vol. 44, no. 1, pp. 276–287.CrossRefPubMedGoogle Scholar
  19. 19.
    Blois, M.S., Antioxidant determinations by the use of a stable free radical, Nature, 1958, vol. 181, pp. 1199–1200.CrossRefGoogle Scholar
  20. 20.
    De Maria, S., Puschhenreiter, M., and Rivelli, A.R., Cadmium accumulation and physiological response of sunflower plants to Cd during the vegetative growing cycle, Plant Soil Environ., 2013, vol. 59, no. 6, pp. 254–261.Google Scholar
  21. 21.
    Mishra, S. and Agrawal, S.B., Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L., Braz. J. Plant Physiol., 2006, vol. 18, no. 2, pp. 307–314.CrossRefGoogle Scholar
  22. 22.
    Chamseddine, M., Wided, B.A., Guy, H., Marie-Edith, C., and Fatma, J., Cadmium and cooper induction of oxidative stress and antioxidative response in tomato (Solanum lycopersicon) leaves, Plant Growth Regul., 2009, vol. 57, no. 1, pp. 89–99.CrossRefGoogle Scholar
  23. 23.
    Schutzendubel, A., Schwanz, P., Teichman, T., Gross, K., Langenfeld-Heyser, R., Godbold, A., and Polle, A., Cadmium-induced changes in antioxidative system H2O2 content and differentiation in Scots pine (Pinus sylvestris) roots, Plant Physiol., 2001, vol. 127, pp. 887–898.CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Márquez-García, B., Fernández-Recamales, M.Á., and Cordoba, F., Effects of cadmium on phenolic composition and antioxidant activities of Erica andevalensis, J. Bot., 2012, vol. 2012, art. ID 936950. doi 10.1155/2012/936950Google Scholar
  25. 25.
    Wojdylo, A., Oszmianski, J., and Czemerys, R., Antioxidant activity and phenolic compounds in 32 selected herbs, Food Chem., 2007, vol. 105, no. 3, pp. 940–949.CrossRefGoogle Scholar
  26. 26.
    Okem, A., Stirk, W.A., Street, R.A., Southway, C., Finnie, J.F., and van Staden, J., Effects of Cd and Al stress on secondary metabolites, antioxidant and antibacterial activity of Hypoxis hemerocallidea Fisch. & C.A. Mey, Plant Physiol. Biochem., 2015, vol. 97, pp. 147–155.Google Scholar
  27. 27.
    Fariss, M.W., Cadmium toxicity: unique cytoprotective properties of alpha tocopheryl succinate in hepatocytes, Toxicology, 1991, vol. 69, no. 1, pp. 63–77.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  1. 1.Department of Bioengineering, Faculty of EngineeringKaramanoğlu Mehmetbey UniversityKaramanTurkey
  2. 2.Department of Biology, Faculty of Science and ArtsGaziosmanpaşa UniversityTokatTurkey
  3. 3.Department of Chemistry, Faculty of Science and ArtsGaziosmanpaşa UniversityTokatTurkey

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