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Russian Journal of Plant Physiology

, Volume 64, Issue 6, pp 861–868 | Cite as

Salinity–induced modulations in the protective defense system and programmed cell death in Nostoc muscorum

  • A. Shamim
  • A. Farooqui
  • M. H. Siddiqui
  • S. Mahfooz
  • J. Arif
Research Papers
  • 52 Downloads

Abstract

To study the biochemical adaptive responses of the blue green algae Nostoc muscorum to the salinity- induced stress they were exposed to various concentrations (5, 10, 15, 20 or 200 mM) of sodium chloride (NaCl). A dose-dependent inhibition of total protein content showed an adverse effect of NaCl on the growth of N. muscorum. Four-day treatment of NaCl (5–20 mM) progressively increased the content of the total peroxide with subsequent increase of the superoxide dismutase (SOD) activity, proline and total phenol content only up to 10 mM NaCl. Higher concentrations of NaCl caused significant decrease in both the enzymatic and non-enzymatic antioxidants. Induction of two polypeptides of ~29.10 and 40.15 kD as well as upregulation of many polypeptides as compared to control indicates the induction of SOD and dehydrin-like proteins, which supports the theory of adaptation against the salt stress. Furthermore, adaptation of N. muscorum to lower concentrations (5–20 mM) of NaCl was also confirmed by no fragmentation of DNA while DNA fragmentation indicating programmed cell death (PCD) could only be seen at 200 mM NaCl for 12 hours. We hypothesized that proline may confer a positive role to combat salinity stress and the same was confirmed by treatment of the test blue green algae with exogenous proline (1 and 10 μM). The results exhibited 16% reduction in the level of total peroxides, which is a well known oxidative stress marker in the 10 μM proline-treated NaCl group as compared to direct exposure to NaCl.

Keywords

Nostoc muscorum sodium chloride stress total peroxide programmed cell death adaptive response dehydrin 

Abbreviations

chl

chlorophyll

DHN

dehydrin

MDA

malondialdehyde

PCD

programmed cell death

ROS

reactive oxygen species

SDS-PAGE

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

SOD

superoxide dismutase

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References

  1. 1.
    Syiem, B.M. and Nongrum, A.N., Increase in intracellular proline content in Anabaena variabilis during stress conditions, J. Appl. Nat. Sci., 2011, vol. 3, pp. 119–123.Google Scholar
  2. 2.
    Demiral, T. and Türkan, I., Exogenous glycinebetaine affects growth and proline accumulation and retards senescence in two rice cultivars under NaCl stress, Environ. Exp. Bot., 2006, vol. 56, pp. 72–79.CrossRefGoogle Scholar
  3. 3.
    Ma, P. and Liu, J., Leymus chinensis that enhances salt stress tolerance in Saccharomyces cerevisiae: isolation and characterization of a novel plasma membrane intrinsic protein gene, LcPIP1, Appl. Biochem. Biotechnol., 2012, vol. 166, pp. 479–485.CrossRefPubMedGoogle Scholar
  4. 4.
    Affenzeller, M.J., Darehshouri, A., Andosch, A., Lutz, C., and Lutz, M.U., Salt stress-induced cell death in the unicellular green alga Micrasterias denticulate, J. Exp. Bot., 2009, vol. 60, pp. 939–954.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Unsal, N.P., Buyuktuncer, E.D., and Tufekci, M.A., Programmed cell death in plants, J. Cell Mol. Biol., 2005, vol. 4, pp. 9–23.Google Scholar
  6. 6.
    Srivastava, A.K., Bhargava, A.P., and Rai, L.C., Salinity and copper-induced oxidative damage and changes in antioxidative defense system of Anabaena doliolum, World J. Microbiol. Biotechnol., 2005, vol. 22, pp. 1291–1298.CrossRefGoogle Scholar
  7. 7.
    Srivastava, A.K., Bhargava, P., Kumar, A., Rai, L.C., and Neilan, B.A., Molecular characterization and effect of salinity on cyanobacterial community from rice fields of Eastern Uttar Pradesh, India, Saline Syst., 2009, vol. 5:4.CrossRefGoogle Scholar
  8. 8.
    Srivastava, A.K., Assessment of salinity-induced antioxidative defense system of diazotrophic cyanobacterium Nostoc muscorum, J. Microbiol. Biotechnol., 2010, vol. 20, pp. 1506–1512.CrossRefPubMedGoogle Scholar
  9. 9.
    Huges, E.O. and Garham, P.R., A toxicity of a unialgal culture of Microcystis aeruginosa, Can. J. Microbiol., 1958, vol. 4, pp. 215–236.CrossRefGoogle Scholar
  10. 10.
    Lowry, O.H., Rosenbrough, N.J., Farr, A.L., and Randall, R.J., Protein measurement with the Folin phenol reagent, J. Biol. Chem., 1951, vol. 193, pp. 269–275.Google Scholar
  11. 11.
    Mackinney, G., Absorption of light by chlorophyll solutions, J. Biol. Chem., 1941, vol. 140, pp. 315–322.Google Scholar
  12. 12.
    Giannopolitis, C.N. and Ries, S.K., Superoxide dismutase. I. Occurrence in higher plants, Plant Physiol., 1977, vol. 59, pp. 309–314.CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Sagisaka, S., The occurrence of peroxide in a perennial plant, Populus gelrica, Plant Physiol., 1976, vol. 57, pp. 308–309.CrossRefPubMedGoogle Scholar
  14. 14.
    Heath, R.L. and Packer, L., Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation, Arch. Biochem. Biophys., 1968, vol. 125, pp. 189–198.CrossRefPubMedGoogle Scholar
  15. 15.
    Bates, L.S., Waldren, R.P., and Teare, I.D., Rapid determination of free proline for water stress studies, Plant Soil, 1975, vol. 39, pp. 205–207.CrossRefGoogle Scholar
  16. 16.
    Singleton, V.L. and Rossi, J.A., Colorimetry of total phenolics with phosphor molybdic-phosphotungstic acid reagents, Am. J. Enol. Viticult., 1965, vol. 16, pp. 144–158.Google Scholar
  17. 17.
    Ivleva, N.B. and Golden, S.S., Circadian rhythms: methods and protocols, Meth. Mol. Biol., 2008, vol. 362, pp. 365–373.CrossRefGoogle Scholar
  18. 18.
    Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.CrossRefPubMedGoogle Scholar
  19. 19.
    Sambrook, J. and Russell, D., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 2001.Google Scholar
  20. 20.
    Whitton, B.A. and Potts, M., The ecology of cyanobacteria: their diversity in time and space, New York: Kluwer, 2000.Google Scholar
  21. 21.
    Tang, D., Shi, S., Li, D., Hua, C., and Liu, Y., Physiological and biochemical responses of Scytonema javanicum (cyanobacterium) to salt stress, J. Arid. Environ., 2007, vol. 71, pp. 312–320.CrossRefGoogle Scholar
  22. 22.
    Liang, X. and Zhang, L., Natarajan, S.K., and Becker, D.F., Proline mechanisms of stress survival, Antioxid. Redox Signal., 2013, vol. 19, pp. 998–1011.CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Hayat, S., Hayat, Q., Alyemeni, M.N., Wani, A.S., Pichtel, J., and Ahmad, A., Role of proline under changing environments, Plant Signal. Behav., 2012, vol. 7, pp. 1456–1466.CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Mazid, M., Khan, T.A., and Mohammad, F., Role of secondary metabolites in defense mechanisms of plants, Biol. Med., 2011, vol. 3, pp. 232–249.Google Scholar
  25. 25.
    Apte, S.K. and Bhagwat, A.A., Salinity-stress-induced proteins in two nitrogen-fixing Anabaena strains differentially tolerant to salt, J. Bacteriol., 1989, vol. 171, pp. 909–915.CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Close, T.J. and Lammers, P.J., An osmotic stress protein of cyanobacteria is immunologically related to plant dehydrins, Plant Physiol., 1993, vol. 101, pp. 773–779.CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Ruibal, C., Salamó, I.P., Carballo, V., Castro, A., Bentancor, M., Borsani, O., Szabados, L., and Vidal, S., Differential contribution of individual dehydrin genes from Physcomitrella patens to salt and osmotic stress tolerance, Plant Sci., 2012, vol. 190, pp. 89–102.CrossRefPubMedGoogle Scholar
  28. 28.
    Okuma, E., Soeda, K., Tada, M., and Murata, Y., Exogenous proline mitigates the inhibition of growth of Nicotiana tabacum cultured cell under saline conditions, Soil Sci. Plant Nutr., 2000, vol. 46, pp. 257–263.CrossRefGoogle Scholar
  29. 29.
    Chris, A., Zeeshan, M., Abrahama, G., and Prasad, S.M., Proline accumulation in Cylindrospermum sp., Environ. Exp. Bot., 2006, vol. 57, pp. 154–159.CrossRefGoogle Scholar
  30. 30.
    Khedr, A.H.A., Abbas, M.A., Wahid, A.A.A., Quick, W.P., and Abogadallah, G.M., Proline induces the expression of salt-stress-responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt-stress, J. Exp. Bot., 2003, vol. 392, pp. 2553–2562.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • A. Shamim
    • 2
  • A. Farooqui
    • 1
  • M. H. Siddiqui
    • 1
  • S. Mahfooz
    • 2
  • J. Arif
    • 2
  1. 1.Department of Bioengineering, Faculty of EngineeringIntegral UniversityLucknowIndia
  2. 2.Department of Biosciences, Faculty of Applied SciencesIntegral UniversityLucknowIndia

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