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Relationship between seed quality and changes in conjugated polyamine in plasma membrane purified from wheat embryos during grain ripening

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Abstract

In order to explore the relationship between seed quality and changes in conjugated polyamine in plasma membrane purified from wheat embryos during grain ripening. Plasma membrane (PM) vesicles were isolated from the embryos of ripening wheat (Triticum aestivum L.) grains by the gradient centrifugation method. The contents of polyamines conjugated (covalently and non-covalently) to the PM vesicles were investigated. Results showed that after pollination, from the 22nd to the 32nd day, the embryos of wheat grains underwent dehydration, as judged by the decrease of embryo relative water content (ERWC). During embryo ripening, non-covalently conjugated (NCC)-Spd and NCC-Spm, covalently conjugated (CC)-Put and CC-Spd contents increased markedly, while relative embryo cell vigor (RECV) decreased slightly. The treatment with methylglyoxyl-bis (guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC), inhibited the increases of the NCC-Spd and NCC-Spm contents, enhanced the decrease of RECV simultaneously, and decreased mature seed relative germination potential (RGP). The effects of MGBG were reversed by exogenous Spd and Spm. Phenanthrolin (o–Phen), an inhibitor of transglutaminase (TGase), inhibited the increases of CC-Put and CC-Spd contents, enhanced the decrease of RECV simultaneously, and decreased mature seed RGP. These results suggest that during embryo ripening, the levels of NCC-Spd, NCC-Spm, CC-Put, and CC-Spd increase, and these increases might affect embryo cell vigor and seed germination potential.

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Abbreviations

CC:

covalently conjugated

ERWC:

embryo relative water content

MGBG:

methylglyoxyl-bis (guanylhydrazone)

NCC:

noncovalently conjugated

o–Phen:

phenanthrolin

PA:

polyamine

PCA:

perchloric acid

PM:

plasma membrane

Put:

putrescine

PVP:

polyvinyl pyrrolidone

RECV:

relative embryo cell vigor

RGP:

relative germination potential

SAMDC:

S-adenosylmethionine decarboxylase

Spd:

spermidine

Spm:

spermine

TGase:

transglutaminase

TTC:

triphenyltetrazolium chloride

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Authors and Affiliations

  1. College of Life Science and Agronomy/Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou Henan, 466001, China

    H. Y. Du, H. P. Liu & R. Kurtenbach

  2. College of biological Science, China Agricultural University, Beijing, 100193, China

    H. Y. Du & G. T. Liu

  3. Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang Henan, 453003, China

    X. G. Zhou

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  1. H. Y. Du
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  2. X. G. Zhou
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  3. G. T. Liu
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Correspondence to H. P. Liu.

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Du, H.Y., Zhou, X.G., Liu, G.T. et al. Relationship between seed quality and changes in conjugated polyamine in plasma membrane purified from wheat embryos during grain ripening. Russ J Plant Physiol 64, 325–332 (2017). https://doi.org/10.1134/S1021443717030074

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  • DOI: https://doi.org/10.1134/S1021443717030074

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