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Plants water soluble chlorophyll binding proteins act as enzyme-inhibitor pair

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Abstract

The hydrophilic water-soluble chlorophyll binding proteins (WSCP) which form complex with chlorophyll molecules have been numerously isolated from the chloroplasts of plants. Although, their molecular properties have been partly characterized, but their physio-biochemical roles are still unclear in the photosynthesizing organs. In this study, using bioinformatic tools WSCP pair were predicted to act as hydrolase and hydrolase inhibitor towards chlorophyll molecules. To enhance our information regarding the possible functions of WSCP, we cloned WSCP1 and WSCP2 cDNAs from Chenopodium album L. and Brassica oleracea L. leaves and expressed them as soluble maltose-binding fusion proteins in Escherichia coli. The purified fused products were subjected to chlorophyll hydrolyzing activity in vitro. The results showed that WSCP1 and WCSP2 are antagonistically involved in chlorophyll breakdown, while WSCP1 acts as chlorophyll hydrolyzing enzyme (with the hydrolysis rate of about 40% per 12 h), WSCP2 exerts inhibitory activity (with the inhibition rate of about 38% per 12 h) towards chlorophyll hydrolysis. This is the first ever time report speculates the hydrolase/inhibitory roles for WSCP and proposes that the relative activity of WSCP pair might balance and regulate the chlorophyll breakdown process in the photosynthetic apparatus of plants. It may open the new gate to investigate the potent roles of WSCP in plant system.

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Abbreviations

BCIP:

5-bromo-1-chloro-3-indolyl phosphate

BPI:

bactericidal permeability increasing

DMF:

dimethylformamide

DUF:

domain of unknown function

IPTG:

isopropyl beta-D-1-thiogalactopyranoside

MBP:

maltose binding protein

NBT:

nitro blue tetrazolium

TBS:

Tris-buffered saline

WSCP:

water soluble chlorophyll binding proteins.

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Authors and Affiliations

  1. Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Tabriz, Iran

    A. Gholizadeh

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  1. A. Gholizadeh
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Correspondence to A. Gholizadeh.

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Gholizadeh, A. Plants water soluble chlorophyll binding proteins act as enzyme-inhibitor pair. Russ J Plant Physiol 64, 91–99 (2017). https://doi.org/10.1134/S1021443717010095

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