Total phenol content and total antioxidant activity drop during Tacitus bellus direct shoot organogenesis
- 81 Downloads
Changes in total phenolics content (TPC) and total antioxidant activity (TAA) in different morphogenic stages of Tacitus bellus (L.) Moran and J. Meyrán (Crassulaceae) direct shoot organogenesis in vitro were examined. A HPLC method was used for the assessment of changes in phenolic profile. Significant decrease in TPC and TAA, as well as the decline of the amount of specific phenolic compounds coincided with the initiation of shoot organogenesis. Additional decrease in TPC and TAA, and the disappearance of procyanidin glycoside, occurred associated with shoot development. Performed correlation analysis suggests phenolics as the main component of nonenzymatic antioxidant system involved in T. bellus direct shoot organogenesis, it argues in favor of involvement of specific phenolics in the regulation of early stages of T. bellus direct shoot organogenesis and indicates different fine regulatory mechanisms of early and late stages of shoot organogenesis.
KeywordsTacitus bellus phenolics shoot organogenesis total antioxidant activity
total antioxidant activity
total phenolics content
Unable to display preview. Download preview PDF.
- 11.Agarwal, M. and Kamal, R., Studies on flavonoid production using in vitro cultures of Momordica charantia L., Ind. J. Biotechnol., 2007, vol. 6, pp. 277–279.Google Scholar
- 12.Shilpashree, H.P. and Raim, R., In vitro plant regeneration and accumulation of flavonoids in Hypericum mysorense, Int. J. Integr. Biol., 2009, vol. 8, pp. 43–49.Google Scholar
- 17.Singleton, V.L. and Rossi, J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Vitic., 1965, vol. 16, pp. 144–158.Google Scholar
- 19.Cheng, Z.H., Wang, L., Sun, W., Zhang, Y., Zhou, C., Sum, Y.H., Li, W., Sun, T.T., Zhao, X.Y., Li, X.G., Cheng, Y., Zhao, Y., Xie, Q., and Zhang, X.S., Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3, Plant Physiol., 2013, vol. 161, pp. 240–251.PubMedCentralCrossRefPubMedGoogle Scholar