Russian Journal of Plant Physiology

, Volume 62, Issue 4, pp 524–533 | Cite as

Participation of cytokinins in plant response to competitors

  • T. N. Arkhipova
  • L. B. Vysotskaya
  • E. V. Martinenko
  • I. I. Ivanov
  • G. R. Kudoyarova
Research Papers


An attempt was made to look into participation of cytokinins in regulation of stomatal behavior and growth response of plants to the presence of competitors in the neighborhood. The plants of tobacco (Nicotiana tabacum L.) and lettuce (Lactuca sativa L.) were accommodated by one or several plants per pot. In rival plants growing by two or three per pot, the level of transpiration considerably fell, accumulation of shoot biomass decelerated, and leaf area decreased as compared with single plants. Reduction in transpiration and plant biomass was accompanied by a decrease in the content of endogenous cytokinins. When the plants were grown in the presence of competitors for a long time, an increase in the content of hormones in transgenic tobacco plants as a result of heat induction of ipt-gene and inoculation of lettuce plants with bacteria Bacillus subtilis, strain IB-22, producing cytokinin, reduced suppression of transpiration and shoot growth in competing plants. This suggests that cytokinins participate in late stages of plant adaptation to competitors.


Nicotiana tabacum Lactuca sativa Bacillus subtilis growth competition cytokinins 



heat shock


total moisture capacity


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  1. 1.
    Pierik, R., Sasidharan, R., and Voesenek, L.A.C.J., Growth control by ethylene: adjusting phenotypes to the environment, J. Plant Growth Regul., 2007, vol. 26, pp. 188–200.CrossRefGoogle Scholar
  2. 2.
    Vysotskaya, L.B. and Kudoyarova, G.R., Role of ethylene in growth and stomatal response of lettuce plants in common growth, Fiziol. Biokh. Kul’t. Rast., 2011, vol. 43, pp. 143–148.Google Scholar
  3. 3.
    Vysotskaya, L., Wilkinson, S., Davies, W.J., Arkhipova, T., and Kudoyarova, G., The effect of competition from neighbours on stomatal conductance in lettuce and tomato plants, Plant Cell Environ., 2011, vol. 34, pp. 729–737.PubMedCrossRefGoogle Scholar
  4. 4.
    Boccalandro, H., Ploschuk, E.L., Yanovsky, M.J., Sanchez, R.A., Gatz, C., and Casal, J.J., Increased phytochrome B alleviates density effects on tuber yield of field potato crops, Plant Physiol., 2003, vol. 133, pp. 1539–1546.PubMedCentralPubMedCrossRefGoogle Scholar
  5. 5.
    Deng, J., Zuo, W., Wang, Z., Fan, Z., Ji, M., Wang, G., Ran, J., Zhao, C., Liu, J., Niklas, K.J., Hammond, S.T., and Brown, J.H., Insights into plant size-density relationships from models and agricultural crops, Proc. Natl. Acad. Sci. USA, 2012, vol. 109, pp. 8600–8605.PubMedCentralPubMedCrossRefGoogle Scholar
  6. 6.
    Forster, M.A., Ladd, B., and Bonser, S.P., Optimal allocation of resources in response to shading and neighbours in the heteroblastic species, Acacia implexa, Ann. Bot., 2011, vol. 107, pp. 219–228.CrossRefGoogle Scholar
  7. 7.
    Pandey, S.P., Gaquerel, E., Gase, K., and Baldwin, I.T., RNA-directed RNA polymerase3 from Nicotiana attenuata is required for competitive growth in natural environments, Plant Physiol., 2008, vol. 147, pp. 1212–1224.PubMedCentralPubMedCrossRefGoogle Scholar
  8. 8.
    Davies, W.J., Kudoyarova, G.R., and Hartung, W., Long-distance ABA signaling and its relation to other signaling pathways in the detection of soil drying and the mediation of the plant’s response to drought, J. Plant Growth Regul., 2005, vol. 24, pp. 285–295.CrossRefGoogle Scholar
  9. 9.
    Mok, D.W.S. and Mok, M.C., Cytokinin metabolism and action, Annu. Rev. Plant Physiol. Plant Mol. Biol., 2001, vol. 52, pp. 89–118.PubMedCrossRefGoogle Scholar
  10. 10.
    Carabelli, M., Possenti, M., Sessa, G., Ciolfi, A., Sassi, M., Morelli, G., and Ruberti, I., Canopy shade causes a rapid and transient arrest in leaf development through auxin-induced cytokinin oxidase activity, Genes Dev., 2007, vol. 21, pp. 1863–1868.PubMedCentralPubMedCrossRefGoogle Scholar
  11. 11.
    Kiba, T., Kudo, T., Kojima, M., and Sakakibara, H., Hormonal control of nitrogen acquisition: roles of auxin, abscisic acid, and cytokinin, J. Exp. Bot., 2011, vol. 62, pp. 1399–1409.PubMedCrossRefGoogle Scholar
  12. 12.
    Schmülling, T., Beinsberger, S., de Greef, J., Shell, J., van Onckelen, H., and Spena, A., Construction of a heat-inducible chimaeric gene to increase the cytokinin content in transgenic plant tissue, FEBS Lett., 1989, vol. 249, pp. 401–406.CrossRefGoogle Scholar
  13. 13.
    Veselova, S.V., Farhutdinov, R.G., Veselov, S.Yu., Kudoyarova, G.R., Veselov, D.S., and Hartung, W., The effect of root cooling on hormone content, leaf conductance and root hydraulic conductivity of durum wheat seedlings (Triticum durum L.), J. Plant Physiol., 2005, vol. 162, pp. 21–26.PubMedCrossRefGoogle Scholar
  14. 14.
    Arkhipova, T.N., Veselov, S.Yu., Melentiev, A.I., Martynenko, E.V., and Kudoyarova, G.R., Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants, Plant Soil, 2005, vol. 272, pp. 201–209.CrossRefGoogle Scholar
  15. 15.
    Vysotskaya, L.B., Kudoyarova, G.R., Veselov, S.Yu., and Jones, H.G., Unusual stomatal behaviour on partial root excision in wheat seedlings, Plant Cell Environ., 2004, vol. 27, pp. 69–77.CrossRefGoogle Scholar
  16. 16.
    Veselov, S.Yu., Ispol’zovanie antitel dlya kolichestvennogo opredeleniya, ochistki i lokalizatsii regulyatorov rosta rastenii (Use of Antibodies for the Quantitative Determination, Purification and Localization of Plant Growth Regulators), Ufa: Bashkir. Gos. Univ., 1998.Google Scholar
  17. 17.
    Wintermans, J.F.G.M. and de Mots, A., Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol, Biochem. Biophys. Acta, 1965, vol. 109, pp. 448–453.PubMedGoogle Scholar
  18. 18.
    Wilkinson, S., Kudoyarova, G.R., Veselov, D.S., Arkhipova, T.N., and Davies, W.J., Plant hormone interactions: innovative targets for crop breeding and management. Review, J. Exp. Bot., 2012, vol. 63, pp. 3499–3509.PubMedCrossRefGoogle Scholar
  19. 19.
    Alwerdt, J.L., Gibson, D.J., Ebbs, S.D., and Wood, A.J., Intraspecific interactions in Arabidopsis thaliana and the stomatal mutants tmm1-1 and sdd1-2, Biol. Plant., 2006, vol. 50, pp. 205–209.CrossRefGoogle Scholar
  20. 20.
    Ramirez, D.A., Querejeta, J.I., and Bellots, J., Bulk leaf δ18O and δ13C reflect the intensity of intraspecific competition for water in a semi-arid tussock grassland, Plant Cell Environ., 2009, vol. 32, pp. 1346–1356.PubMedCrossRefGoogle Scholar
  21. 21.
    Wilkinson, S., Bacon, M.A., and Davies, W.J., Nitrate signalling to stomata and growing leaves: interactions with soil drying, ABA and xylem sap pH in maize, J. Exp. Bot., 2007, vol. 58, pp. 1705–1716.PubMedCrossRefGoogle Scholar
  22. 22.
    Batasheva, S.N., Abdrakhimov, F.A., Bakirova, G.G., and Chikov, V.I., Effect of nitrates supplied with the transpiration flow on assimilate translocation, Russ. J. Plant Physiol., 2007, vol. 54, pp. 373–380.CrossRefGoogle Scholar
  23. 23.
    Lu, P., Outlaw, W.H., Jr., Smith, B.G., and Freed, G.A., A new mechanism for the regulation of stomatal aperture size in intact leaves (accumulation of mesophyll-derived sucrose in the guard-cell wall of Vicia faba), Plant Physiol., 1997, vol. 114, pp. 109–118.PubMedCentralPubMedGoogle Scholar
  24. 24.
    Kudoyarova, G.R., Vysotskaya, L.B., Cherkozyanova, A., and Dodd, I.C., Effect of partial rootzone drying on the concentration of zeatin-type cytokinins in tomato (Solanum lycopersicum L.) xylem sap and leaves, J. Exp. Bot., 2007, vol. 58, pp. 161–168.PubMedCrossRefGoogle Scholar
  25. 25.
    Vysotskaya, L.B., Korobova, A.V., Veselov, S.Yu., Dodd, I.C., and Kudoyarova, G.R., ABA mediation of shoot cytokinin oxidase activity: assessing its impacts on cytokinin status and biomass allocation of nutrient deprived durum wheat, Funct. Plant Biol., 2009, vol. 36, pp. 66–72.CrossRefGoogle Scholar
  26. 26.
    Vysotskaya, L.B., Veselov, S.Yu., and Kudoyarova, G.R., Effect on shoot water relations, and cytokinin and abscisic acid levels of inducing expression of a gene coding for isopentenyltransferase in roots of transgenic tobacco plants, J. Exp. Bot., 2010, vol. 61, pp. 3709–3717.PubMedCrossRefGoogle Scholar
  27. 27.
    Werner, T., Motyka, V., Laucou, V., Smets, R., van Onckelen, H., and Schmülling, T., Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity, Plant Cell, 2003, vol. 15, pp. 2532–2550.PubMedCentralPubMedCrossRefGoogle Scholar
  28. 28.
    Tarakanov, I.G. and Wang, J., Light trophic and signal roles in the control of morphogenesis of the Brassica plants developing storage roots, Russ. J. Plant Physiol., 2009, vol. 56, pp. 232–241.CrossRefGoogle Scholar
  29. 29.
    Teplova, I.R., Farkhutdinov, R.G., Mitrichenko, A.N., Ivanov, I.I., Veselov, S.Yu., Valcke, R.L., and Kudoyarova, G.R., Response of tobacco plants transformed with the ipt gene to elevated temperature, Russ. J. Plant Physiol., 2000, vol. 47, pp. 367–369.Google Scholar
  30. 30.
    Arkhipova, T.N., Prinsen, E., Veselov, S.Yu., Martinenko, E.V., Melentiev, A.I., and Kudoyarova, G.R., Cytokinin producing bacteria enhance plant growth in drying soil, Plant Soil, 2007, vol. 292, pp. 305–315.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • T. N. Arkhipova
    • 1
  • L. B. Vysotskaya
    • 1
  • E. V. Martinenko
    • 1
  • I. I. Ivanov
    • 1
  • G. R. Kudoyarova
    • 1
  1. 1.Institute of Biology, Ufa Scientific CenterRussian Academy of SciencesUfaRussia

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