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Effect of laser irradiation and ethylene on chilling tolerance of wheat seedlings

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Abstract

In order to determine the mechanism of laser-enhanced chilling tolerance in wheat (Triticum aestivum L.) seedlings, the seeds were exposed to different treatments, and some physiological and biochemical parameters were measured in 5-day-old seedlings. The results showed that in wheat seedlings subjected to 1-aminocyclopropane-1-carboxylic acid (ACC) followed by chilling stress (CS), the concentrations of H2O2, O2 .− and MDA were lower, while the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as the soluble protein content (SPC) and the seedling length (SL) were higher than those in chilling stress treatment. When wheat seedlings were subjected to aminooxyacetic acid (AOA) followed by chilling stress, the results were different from those in which the seedlings were subjected to ACC. Although ACC and AOA treatments followed by laser irradiation led to an increase in the aforementioned enzyme activities, SPC, and SL, with a simultaneous decrease in concentrations of H2O2, O2 .−, and MDA, the effect of ACC treatment followed by laser irradiation was more favorable than that of AOA treatment followed by laser light. Similarly, the oxidative damage induced by chilling stress was alleviated in treatment with ACC+LR and AOA+LR, but alleviation effects of ACC+LR+CS were more prominent than in the treatment AOA+LR+CS. These results suggest that the laser light and ethylene have a positive synergistic effect, thus enhancing chilling tolerance in wheat seedlings.

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Abbreviations

ACC:

1-aminocyclopropane-1-carboxylic acid

AOA:

aminooxyacetic acid

APX:

ascorbate peroxidase

CAT:

catalase

CS:

chilling stress

GR:

glutathione reductase

LR:

laser radiation

POD:

peroxidases

ROS:

reactive oxygen species

SL:

shoot length

SOD:

superoxide dismutase

SPC:

soluble protein content

ACC+CS:

treatment in which the seeds were incubated in the presence of ACC and then exposed to chilling stress

ACC+LR:

seeds were treated with ACC followed by exposure to LR

ACC+LR+CS:

seeds were treated with ACC followed by exposure to LR and CS

AOA+CS:

seeds were treated with AOA followed by CS

AOA+LR:

seeds were treated with AOA followed by exposure to LR

AOA+LR+CS:

seeds were treated with AOA followed by exposure to LR and CS

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Authors and Affiliations

  1. SKLLQG, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an, 710075, China

    Y. P. Chen & Q. Liu

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  1. Y. P. Chen
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Chen, Y.P., Liu, Q. Effect of laser irradiation and ethylene on chilling tolerance of wheat seedlings. Russ J Plant Physiol 62, 299–306 (2015). https://doi.org/10.1134/S1021443715030048

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  • DOI: https://doi.org/10.1134/S1021443715030048

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