Skip to main content
SpringerLink
Log in

Effect of laser irradiation and ethylene on chilling tolerance of wheat seedlings

  • Research Papers
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

In order to determine the mechanism of laser-enhanced chilling tolerance in wheat (Triticum aestivum L.) seedlings, the seeds were exposed to different treatments, and some physiological and biochemical parameters were measured in 5-day-old seedlings. The results showed that in wheat seedlings subjected to 1-aminocyclopropane-1-carboxylic acid (ACC) followed by chilling stress (CS), the concentrations of H2O2, O2 .− and MDA were lower, while the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as the soluble protein content (SPC) and the seedling length (SL) were higher than those in chilling stress treatment. When wheat seedlings were subjected to aminooxyacetic acid (AOA) followed by chilling stress, the results were different from those in which the seedlings were subjected to ACC. Although ACC and AOA treatments followed by laser irradiation led to an increase in the aforementioned enzyme activities, SPC, and SL, with a simultaneous decrease in concentrations of H2O2, O2 .−, and MDA, the effect of ACC treatment followed by laser irradiation was more favorable than that of AOA treatment followed by laser light. Similarly, the oxidative damage induced by chilling stress was alleviated in treatment with ACC+LR and AOA+LR, but alleviation effects of ACC+LR+CS were more prominent than in the treatment AOA+LR+CS. These results suggest that the laser light and ethylene have a positive synergistic effect, thus enhancing chilling tolerance in wheat seedlings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ACC:

1-aminocyclopropane-1-carboxylic acid

AOA:

aminooxyacetic acid

APX:

ascorbate peroxidase

CAT:

catalase

CS:

chilling stress

GR:

glutathione reductase

LR:

laser radiation

POD:

peroxidases

ROS:

reactive oxygen species

SL:

shoot length

SOD:

superoxide dismutase

SPC:

soluble protein content

ACC+CS:

treatment in which the seeds were incubated in the presence of ACC and then exposed to chilling stress

ACC+LR:

seeds were treated with ACC followed by exposure to LR

ACC+LR+CS:

seeds were treated with ACC followed by exposure to LR and CS

AOA+CS:

seeds were treated with AOA followed by CS

AOA+LR:

seeds were treated with AOA followed by exposure to LR

AOA+LR+CS:

seeds were treated with AOA followed by exposure to LR and CS

References

  1. Morsy, M.R., Jouve, L., Hausman, J.F., Hoffmann, L., and Stewart, J.M., Alteration of oxidative and carbohy-drate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance, J. Plant Physiol., 2007, vol. 164, pp. 157–167.

    Article  CAS  PubMed  Google Scholar 

  2. Wang, C.Y. and Adams, D.O., Chilling-induced ethylene production in cucumbers (Cucumis sativus L.), Plant Physiol., 1982, vol. 69, pp. 424–427.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Kacperska, A., Ethylene synthesis and a role in plant responses to different stressors, Biology and Biotechnology of the Plant Hormone Ethylene, Kanellis, A.K., Chang, C., Kende, H., Grierson, D., Eds., Dordrecht: Kluwer Academic, 1997, pp. 207–216.

    Chapter  Google Scholar 

  4. Yang, S.F. and Hoffman, N.E., Ethylene biosynthesis and its regulation in higher plants, Annu. Rev. Plant Physiol., 1984, vol. 35, pp. 155–189.

    Article  CAS  Google Scholar 

  5. Nandwal, A.S., Maan, A., Kundu, B.S., Sheokand, S., Kamboj, D.V., Sheoran, A., Kumar, B., and Dutta, D., Ethylene evolution and antioxidant defence mechanism in Cicer arietinum roots in the presence of nitrate and aminoethoxyvinylglycine, Plant Physiol. Biochem., 2000, vol. 38, pp. 709–715.

    Article  CAS  Google Scholar 

  6. Larrigaudiere, C., Vilaplana, R., Soria, Y., and Recasens, I., Oxidative behaviour of Blanquilla pears treated with 1-methylcyclopropene during cold storage, J. Sci. Food Agric., 2004, vol. 84, pp. 1871–1877.

    Article  CAS  Google Scholar 

  7. Lee, D.H. and Lee, C.B., Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays, Plant Sci., 2000, vol. 159, pp. 75–85.

    Article  CAS  PubMed  Google Scholar 

  8. Djanaguiraman, M., Sheeba, J.A., Devi, D.D., Bangarusamy, U., and Prasad, P.V.V., Nitrophenolates spray can alter boll abscission rate in cotton through enhanced peroxidase activity and increased ascorbate and phenolics levels, J. Plant Physiol., 2010, vol. 167, pp. 1–9.

    Article  CAS  PubMed  Google Scholar 

  9. Ramasarma, T., Many faces of superoxide dismutase, originally known as erythrocuprein, Curr. Sci., 2007, vol. 92, pp. 184–191.

    CAS  Google Scholar 

  10. Smirnoff, N., The role of active oxygen in the response of plants to water deficit and desiccation, New Phytol., 1993, vol. 125, pp. 27–58.

    Article  CAS  Google Scholar 

  11. Corpas, F.J., Palma, J.M., Sandalio, L.M., Lopez-Huertas, E., Romero-Puertas, M.C., Barroso, J.B., and Del Río, L.A., Purification of catalase from pea leaf peroxisomes: identification of five different isoforms, Free Radic. Res., 1999, vol. 31, pp. 235–241.

    Article  Google Scholar 

  12. Kawano, T., Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction, Plant Cell Rep., 2003, vol. 21, pp. 829–837.

    CAS  PubMed  Google Scholar 

  13. Sakihama, Y., Cohen, M.F., Grace, S.C., and Yamasaki, H., Plant phenolic antioxidant and peroxidant activities: phenolics-induced oxidative damage mediated by metals in plants, Toxicology, 2002, vol. 177, pp. 67–80.

    Article  CAS  PubMed  Google Scholar 

  14. Perveen, R., Jamil, Y., Ashraf, M., Ali, Q., Iqbal, M., and Ahmad, M.R., He-Ne laser-induced improvement in biochemical, physiological, growth and yield characteristics in sunflower (Helianthus annuus L.), Photochem. Photobiol., 2011, vol. 87, pp. 1453–1463.

    Article  CAS  PubMed  Google Scholar 

  15. Qiu, Z.B., Liu, X., Tian, X.J., and Yue, M., Effects of CO2 laser pretreatment on drought stress resistance in wheat, Photochem. Photobiol., 2008, vol. 90, pp. 17–25.

    Article  CAS  Google Scholar 

  16. Chen, Y.P., Jia, J.F., and Yue, M., Effect of CO2 laser radiation on physiological tolerance of wheat seedlings exposed to chilling stress, Photochem. Photobiol., 2010, vol. 86, pp. 600–605.

    Article  CAS  PubMed  Google Scholar 

  17. Predieri, S., Norman, H.A., Krizek, D.T., Pillai, P., Mirecki, R.M., and Zimmerman, R.H., Influence of UV-B radiation on membrane lipid composition and ethylene evolution in ‘Doyenne d’Hiver’ pear shoots grown in vitro under different photosynthetic photon fluxes, Environ. Exp. Bot., 1995, vol. 35, pp. 151–160.

    Article  CAS  Google Scholar 

  18. Lin, Z.F., The accumulation of hydrogen peroxide in senescing leaves and chloroplasts in relation to lipid peroxidation, Acta Photophysiol. Sin., 1988, vol. 14, pp. 16–22.

    CAS  Google Scholar 

  19. Gao, J.F., Method of determining O2 .- in plant, Technology of Plant Physiological Experiment,Beijing: High Education Press, 2001.

    Google Scholar 

  20. Giannopolitis, C.N. and Ries, S.K., Superoxide dismutase. II. Purification and quantitative relationship with water-soluble protein in seedlings, Plant Physiol., 1977, vol. 59, pp. 315–318.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Nakano, Y. and Asada, K., Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts, Plant Cell Physiol., 1981, vol. 22, pp. 867–880.

    CAS  Google Scholar 

  22. Cakmak, I. and Marschner, H., Magnesium deficiency and high light intensity enhance activity of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves, Plant Physiol., 1992, vol. 98, pp. 1222–1227.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  23. Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding, Anal. Biol. Chem., 1976, vol. 72, pp. 248–254.

    CAS  Google Scholar 

  24. Hoffman, N.E., Liu, Y., and Yang, S.F., Changes in 1-(malonylamino)-cyclopropane-L-carboxylic acid in wilted wheat leaves in relation to their ethylene production rates and L-aminocyclopropane-L-carboxylic acid content, Planta, 1983, vol. 157, pp. 518–523.

    Article  CAS  PubMed  Google Scholar 

  25. Chen, Y.Z. and Patte, B.D., Ethylene and L-aminocyclopropane-L-carboxylic acid as indicator of chilling sensitivity in various plant species, Aust. J. Plant Physiol., 1985, vol. 12, pp. 377–385.

    Article  Google Scholar 

  26. Wang, H.H., Liang, X.L., Wan, Q., Wang, X.M., and Bi, Y.R., Ethylene and nitric oxide are involved in maintaining ion homeostasis in Arabidopsis callus under salt stress, Planta, 2009, vol. 230, pp. 293–307.

    Article  CAS  PubMed  Google Scholar 

  27. Achard, P., Cheng, H., Grauwe, D.L., Decat, J., Schoutteten, H., Moritz, T., Straeten, D.V.D., Peng, J.R., and Harberd, N.P., Integration of plant responses to environmentally activated phytohormonal signals, Science, 2006, vol. 311, pp. 91–94.

    Article  CAS  PubMed  Google Scholar 

  28. Jung, J.Y., Shin, R., and Schachtman, D.P., Ethylene mediates response and tolerance to potassium deprivation in Arabidopsis, Plant Cell, 2009, vol. 21, pp. 607–621.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Baker, A.J.M. and Walker, P.I., Physiological responses of plants to heavy metals and the quantification of tolerance and toxicity, Chem. Spec. Bioavailab., 1989, vol. 1, pp. 7–17.

    CAS  Google Scholar 

  30. Breckle, S.W. and Kahle, H., Effect of toxic heavy metals (Cd, ) on growth and mineral nutrition of beech (Fagus sylvatica L.), Vegetation, 1992, vol. 101, pp. 43–53.

    Article  Google Scholar 

  31. Chen, Y.P., Liu, Y.J., Wang, X.L., Ren, Z.Y., and Yue, M., Effect of microwave and He-Ne laser on enzyme activity and biophoton emission of Isatis indigotica Fort, J. Integr. Plant Biol., 2005, vol. 47, pp. 849–855.

    Article  CAS  Google Scholar 

  32. Liburdy, R.P. and Penn, A., Microwave bioeffects in erythrocytes are temperature and pO2 dependent: cation permeability and protein shedding occur at the membrane phase transition, Bioelectromagnetics, 1984, vol. 5, pp. 283–291.

    Article  CAS  PubMed  Google Scholar 

  33. Daniel, R.M., The upper limits of enzyme thermal stability, Enzyme Microb. Technol., 1996, vol. 19, pp. 74–79.

    Article  CAS  Google Scholar 

  34. Yan, L.F. and Zhang, Y.L., Molecular Biology, Beijing: China Agricultural University Press, 1997 [in Chinese].

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. SKLLQG, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an, 710075, China

    Y. P. Chen & Q. Liu

Authors
  1. Y. P. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Q. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y. P. Chen.

Additional information

The text was submitted by the authors in English.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, Y.P., Liu, Q. Effect of laser irradiation and ethylene on chilling tolerance of wheat seedlings. Russ J Plant Physiol 62, 299–306 (2015). https://doi.org/10.1134/S1021443715030048

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1021443715030048

Keywords

Search

Navigation