Cytophysiological characteristics of Arabidopsis thaliana cultivated cells with disable perception of ethylene signal by the ETR1 receptor
- 83 Downloads
Contradictory data about ethylene influence on cell growth and division prompted us to investigate cytophysiological characteristics of suspension cultures of Arabidopsis thaliana of wild type Col-0 and ert1-1 mutant carrying a point mutation in the site of ethylene binding by the ETR1 receptor. Some cytophysiological characteristics of the etr1-1 cultivated cells differed from those of Col-0: the growth rate of mutant cells was less and cell sizes were smaller, the culture was committed to the formation of tracheary elements (TE), had a pronounced modal class of nuclei (54%) with the amount of DNA 8C and a tendency to expand the ploidy toward 32C. Despite the absence of ethylene perception by the ETR1 receptor, the cell culture of mutant responded to treatment with ethylene by growth acceleration, an increase in cell viability and in the number of cells in the S-phase of the cell cycle. The inhibitor of ethylene binding to receptors, 1-methylcyclopropene, suppressed growth and viability of the cells of both genotypes. In the etr1-1 cell culture, the inhibitor reduced the number of S-phase nuclei and activated TE formation. All data obtained indicate that ethylene perception and transduction of ethylene signal are required for the maintenance of cell viability and active in vitro growth. It is supposed that the functional activity of the ETR1 receptor is necessary for optimal cell expansion, whereas other receptors are responsible for cell proliferation.
KeywordsArabidopsis thaliana etr1-1 ethylene 1-methylcyclopropene cell culture growth S-phase tracheary elements DNA cytophotometry
DNA amount in the diploid chromosome set
phosphate buffered saline
Unable to display preview. Download preview PDF.
- 1.Abeles, F.B., Morgan, P.W., and Salveit, M.E., Ethylene in Plant Biology, San Diego: Academic, 1992.Google Scholar
- 2.Grierson, D., 100 years of ethylene — a personal view, Annu. Plant Rev., 2012, vol. 44, pp. 1–17.Google Scholar
- 10.Binder, B.M., Chang, C., and Schaller, G.E., Perception of ethylene by plants — ethylene receptors, Annu. Plant Rev., 2012, vol. 44, pp. 117–145.Google Scholar
- 12.Qu, X., Hall, B.P., Gao, Z., and Schaller, G.E., A strong constitutive ethylene-response phenotype conferred on Arabidopsis plants containing null mutations in the ethylene receptor ETR1 and ERS1, BMC Plant Biol., 2007, vol. 7, no. 3, doi: 10.1186/1471-2229-7-3Google Scholar
- 13.Moshkov, I.E., Novikova, G.V., Hall, M.A., and George, E.F., Plant growth regulators III: Gibberellins, ethylene, abscisic acid, their analogues and inhibitors; miscellaneous compounds, Plant Propagation by Tissue Culture, vol. 1, The Background, George, E.F., Hall, M.A., and de Klerk, G.J., Eds., Dordrecht: Springer, 2008, pp. 227–281.Google Scholar
- 17.Kotogány, E., Dudits, D., Horváth, G.V., and Ayaydin, F., A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine, Plant Methods, 2010, vol. 6, no. 5, doi: 10.1186/1746-4811-6-5Google Scholar
- 18.Rakitin, V.Yu. and Rakitin, L.Yu., Determination of gas exchange and ethylene, carbon dioxide, and oxygen contents in plant tissues, Sov. Plant Physiol., 1986, vol. 33, pp. 403–413.Google Scholar
- 20.Stepanchenko, N.S., Fomenkov, A.A., Moshkov, I.E., Rakitin, V.Yu., Novikova, G.V., and Nosov, A.V., Phytohormone interplay controls proliferation of in vitro cultivated cells of Arabidopsis thaliana ethylene-insensitive mutants, Dokl. Biol. Sci., 2012, vol. 442, pp. 46–49.PubMedCrossRefGoogle Scholar
- 25.Polko, J.K., Voesenek, L.A.C.J., Peeters, A.J.M., and Pierik, R., Petiole hyponasty: an ethylene-driven, adaptive response to changes in the environment, AoB Plants, 2011: plr031, doi 10.1093/aobpla/plr031Google Scholar