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Responses of the antioxidant defense system to drought stress in the leaves of Fargesia denudata seedlings, the staple food of the giant panda

Abstract

The responses of the antioxidant defense system in plant species to drought stress are still relatively unknown. In order to further understand how the system responds to drought stress, the leaves of Fargesia denudata seedlings were investigated. Antioxidant enzyme activities, antioxidant contents, hydrogen peroxide (H2O2), superoxide anion (O ·−2 ) and MDA contents in the seedling leaves were measured under well-watered (WW), moderate drought-stressed (MD), and severe drought-stressed (SD) treatments. Although drought stress significantly increased H2O2 and O ·−2 levels in F. denudata leaves, only weak lipid peroxidation was observed. This is attributed to the higher superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) activities in F. denudata leaves during the entire drought period. Reduced and oxidized ascorbate (AsA and DHA) contents were almost not affected by drought except that DHA under SD showed an obvious increase on day 30. Furthermore, reduced glutathione (GSH) content under drought stress significantly decreased, while oxidized glutathione (GSSG) markedly increased under SD on days 30 and 45 as well as under MD on day 30; as a result, the ratio GSH/GSSG declined considerably. These results indicated that GSH was involved in scavenging H2O2 and O ·−2 under drought stress and it was more sensitive to drought stress in scavenging H2O2 and O ·−2 than AsA. As a result, a highly efficient antioxidant defense system in drought-stressed F. denudate leaves operated mainly through the synergistic functioning of SOD, CAT, APX, MDHAR, DHAR, GR, and GSH against oxidative damage.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbate

CAT:

catalase

DHA:

ascorbate oxidized form; dehydroascorbate

DHAR:

dehydroascorbate reductase

GR:

glutathione reductase

GSH:

glutathione (reduced form)

GSSG:

glutathione (oxidized form)

MDHAR:

monodehydroascorbate reductase

O ·−2 :

superoxide anion

POD:

peroxidase

SOD:

superoxide dismutase

tAsA:

total ascorbate

tGSH:

total glutathione

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Correspondence to Y. Wang.

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Liu, C., Wang, Y., Pan, K. et al. Responses of the antioxidant defense system to drought stress in the leaves of Fargesia denudata seedlings, the staple food of the giant panda. Russ J Plant Physiol 61, 374–383 (2014). https://doi.org/10.1134/S1021443714020083

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Keywords

  • Fargesia denudata
  • drought stress
  • antioxidant defense system
  • reactive oxygen species
  • lipid peroxidation