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Mitochondrial retrograde regulation of HSP101 expression in Arabidopsis thaliana under heat stress and amiodarone action

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Abstract

Heat stress in plants elevates the potential across the inner mitochondrial membrane (mtΔψ) and activates the expression of heat shock proteins (HSPs). The treatment of Saccharomyces cerevisiae cells with amiodarone (AMD) elevated the cytosolic Ca2+ level ([Ca2+]cyt) in parallel with (mtΔψ) increase and led to the induction of Hsp104 synthesis. The hyperpolarization was presumably due to the increase in [Ca2+]cyt. In the present study the effects of AMD (0–100 μM) on cell viability, HSP expression, mtΔψ, and [Ca2+]cyt were investigated using the cell culture of Arabidopsis thaliana (L.) Heynh. The treatment of cultured cells with AMD led to the elevation of [Ca2+]cyt, which was accompanied by the increase in mtΔψ and by activation of HSP101 expression. The increase in [Ca2+]cyt and expression of HSP101 were also observed upon the treatment with the protonophore CCCP (carbonyl cyanide m-chlorophenylhydrazone, 4 μM) known to diminish mtΔψ. The results suggest that plant cell mitochondria modulate the cytosolic Ca2+ level by changing the potential at the inner mitochondrial membrane and, thereby, participate in the retrograde regulation of HSP101 expression.

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Abbreviations

AMD:

amiodarone (2-butyl-3-(3,5-diiodo-4-diethylaminoethoxybenzoyl) benzofuran)

CCCP:

carbonyl cyanide m-chlorophenylhydrazone

FDA:

fluorescein diacetate

HSP:

heat shock protein

JC:

1-5,5′,6,6′-tetrachloro-1,1′,2,2′-tetraethyl-benzimidazol-carbocyanine

mtΔϖ:

potential across the inner mitochondrial membrane

PI:

propidium iodide

RT:

PCR-reverse transcription polymerase chain reaction

SE:

standard error

TTC:

2,3,5-triphenyltetrazolium chloride

[Ca2+]mit:

concentration of mitochondrial Ca2+

[Ca2+]cyt:

concentration of cytosolic Ca2+

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, ul. Lermontova 132, POB 317, Irkutsk, 664033, Russia

    D. V. Pyatrikas, E. G. Rikhvanov, I. V. Fedoseeva, N. N. Varakina, T. M. Rusaleva, E. L. Tauson, A. V. Stepanov, G. B. Borovskii & V. K. Voinikov

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  1. D. V. Pyatrikas
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  2. E. G. Rikhvanov
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  3. I. V. Fedoseeva
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  4. N. N. Varakina
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  5. T. M. Rusaleva
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  6. E. L. Tauson
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  7. A. V. Stepanov
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  8. G. B. Borovskii
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  9. V. K. Voinikov
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Correspondence to D. V. Pyatrikas.

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Original Russian Text © D.V. Pyatrikas, E.G. Rikhvanov, I.V. Fedoseeva, N.N. Varakina, T.M. Rusaleva, E.L. Tauson, A.V. Stepanov, G.B. Borovskii, V.K. Voinikov, 2014, published} in Fiziologiya Rastenii, 2014, Vol. 61, No. 1, pp. 88–98.

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Pyatrikas, D.V., Rikhvanov, E.G., Fedoseeva, I.V. et al. Mitochondrial retrograde regulation of HSP101 expression in Arabidopsis thaliana under heat stress and amiodarone action. Russ J Plant Physiol 61, 80–89 (2014). https://doi.org/10.1134/S1021443714010117

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