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Strong light elevates thermotolerance of photosynthetic apparatus and the content of membranes and polar lipids in wheat leaves

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Abstract

The influence of excess irradiance on resistance of wheat (Triticum aestivum L.) photosynthetic apparatus to heating in darkness and in the light was investigated and compared with changes in leaf cell ultra-structure and composition of cell lipids and fatty acids. The leaves of 14- to 16-day-old plants grown at low irradiance (about 20 W/m2) were exposed for 1 h to irradiance of 370 or 600 W/m2 PAR. Using infrared gas analysis, we found that the preexposure of leaves to excess irradiation elevated resistance of apparent photosynthesis to 10-min heat treatment at 40–45°C. The rate of Hill reaction (reduction of 2,6-dichlorophenolindophenol by isolated chloroplasts) was higher for leaves heated at high irradiance than for leaves heated in darkness. During illumination of leaves with strong light, mesophyll cells became more abundant in mitochondria and peroxysomes, as well as in cisternae of endoplasmic reticulum and Golgi complex. The chloroplast thylakoids and grana became more extensive and numerous. At the same time, the leaf content of main classes of membrane glycerolipids increased in parallel with the increase in the phospholipid/glycolipid and lipid/chlorophyll ratios. The unsaturation index of fatty acids of membrane lipids increased because of the elevated content of linolenic acid. Thus, excessive light (not fully utilized in photosynthesis) induced in wheat leaves a series of nonspecific adaptive changes that were similar to those occurring under the action of other environmental factors, such as heat shock, cooling, salinity, and osmotic stresses.

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Abbreviations

Chl:

chlorophyll

DCPIP:

2,6-dichlorophenolindophenol

DGDG:

digalactosyldiacylglycerols

ER:

endoplasmic reticulum

FA:

fatty acids

GL:

glycolipids

JA:

jasmonic acid

MGDG:

monogalactosyldiacylglycerols

PC:

phosphatidylcholines

PE:

phosphatidylethanolamines

PG:

phosphatidylglycerols

PI:

phosphatidylinositols

PL:

phospholipids

PSII:

photosystem II

SQDG:

sulfoquinovosyldiacylglycerol

UI:

unsaturation index of fatty acids

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Authors and Affiliations

  1. Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova 2, St. Petersburg, 197376, Russia

    I. M. Kislyuk, L. S. Bubolo, O. D. Bykov, I. E. Kamentseva, E. R. Kotlova & M. A. Vinogradskaya

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  1. I. M. Kislyuk
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  2. L. S. Bubolo
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  3. O. D. Bykov
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  4. I. E. Kamentseva
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  5. E. R. Kotlova
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  6. M. A. Vinogradskaya
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Correspondence to I. M. Kislyuk.

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Original Russian Text © I.M. Kislyuk, L.S. Bubolo, O.D. Bykov, I.E. Kamentseva, E.R. Kotlova, M.A. Vinogradskaya, 2013, published in Fiziologiya Rastenii, 2013, Vol. 60, No. 5, pp. 649–660.

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Kislyuk, I.M., Bubolo, L.S., Bykov, O.D. et al. Strong light elevates thermotolerance of photosynthetic apparatus and the content of membranes and polar lipids in wheat leaves. Russ J Plant Physiol 60, 611–622 (2013). https://doi.org/10.1134/S1021443713050099

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