New microalgal strains that are native to South-East Kazakhstan were isolated and characterized with a view to identifying suitable candidates for biodiesel production. Six strains of chlorophyte algae (named K1–K6) were recovered from environmental samples as axenic cultures, and molecular analysis revealed that five (K1–K5) are strains of Parachlorella kessleri, whereas K6 is a strain of Chlorella vulgaris. A third isolate from Uzbekistan (termed UZ) was also identified as a separate strain of P. kessleri. All strains show high growth rates and an ability to utilize acetate as an exogenous source of fixed carbon. Furthermore, under conditions of nitrogen depletion, all three strains showed a significant accumulation of neutral lipids (triacylglycerides). P. kessleri K5 and C. vulgaris K6 therefore represent promising autochthon strains for large-scale cultivation and biodiesel production in Kazakhstan.
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Field, C.B., Behrenfeld, M.J., Randerson, J.T., and Falkowski, P., Primary production of the biosphere: integrating terrestrial and oceanic components, Science, 1998, vol. 281, pp. 237–240.
Chisti, Y., Biodiesel from microalgae, Biotechnol. Adv., 2007, vol. 25, pp. 294–306.
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., and Darzins, A., Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances, Plant J., 2008, vol. 54, pp. 621–639.
Georgianna, D.R. and Mayfield, S.P., Exploiting diversity and synthetic biology for the production of algal biofuels, Nature, 2012, vol. 488, pp. 329–335.
Larkum, A.W., Ross, I.L., Kruse, O., and Hankamer, B., Selection, breeding and engineering of microalgae for bioenergy and biofuel production, Trends Biotechnol., 2012, vol. 30, pp. 198–205.
Malcata, F.X., Microalgae and biofuels: a promising partnership? Trends Biotechnol., 2011, vol. 29, pp. 542–549.
Tamiya, H., Morimura, M., Yorota, M., and Kunieda, R., Mode of nuclear division in synchronous cultures of Chlorella: comparison of various methods of synchronization, Plant Cell Physiol., 1961, vol. 2, pp. 383–403.
Vasyurenko, Z.P. and Sinyak, K.M., Influence of culture medium of the fatty-acid profile in enteric bacteria, J. Hyg. Epidemiol. Microbiol. Immunol., 1979, vol. 23, pp. 397–406.
Berthold, D.A., Best, B.A., and Malkin, R., A rapid DNA preparation for PCR from Chlamydomonas reinhardtii and Arabidopsis thaliana, Plant Mol. Biol. Rep., 1993, vol. 11, pp. 338–344.
Hall J.D., Fucíková K., Lo, C., Lewis, L.A., and Karol, K.G., An assessment of proposed DNA barcodes in freshwater green algae, Cryptogamie, Algologie, 2010, vol. 31, pp. 529–555.
Gorman, D.S. and Levine, R.P., Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardtii, Proc. Natl. Acad. Sci. USA, 1965, vol. 54, pp. 1665–1669.
Cooksey, K.E., Guckert, J.B., Williams, S.A., and Callis, P.R., Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red, J. Microbiol. Methods, 1987, vol. 6, pp. 333–345.
Ratha, S.K., Babu, S., Renuka, N., Prasanna, R., Prasad, R.B., and Saxena, A.K., Exploring nutritional modes of cultivation for enhancing lipid accumulation in microalgae, J. Basic Microbiol., 2012, doi 10.1002/jobm.201200001
De la Hoz, Siegler, H., Ayidzoe, W., Ben-Zvi, A., Burrell, R.E., and McCaffrey, W.C., Improving the reliability of fluorescence-based neutral lipid content measurements in microalgal cultures, Algal Res., 2012, vol. 1, pp. 176–184.
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Dyo, Y.M., Vonlanthen, S.E., Purton, S. et al. Evaluating new isolates of microalgae from Kazakhstan for biodiesel production. Russ J Plant Physiol 60, 549–554 (2013). https://doi.org/10.1134/S1021443713040031
- Chlorella vulgaris
- Parachlorella kessleri