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Cloning and expression analysis of Chitinase genes from Populus canadensis

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Abstract

Plant chitinases play a key role in conferring resistance to environmental stresses, including attack by fungal pathogens. In the present study, we employed rapid amplification of cDNA ends (RACE) to identify five chitinase genes in Populus canadensis Moench. Sequence alignment revealed that these genes belong to five subfamilies of chitinase genes. The full-length cDNAs of these genes ranged in size from 991 to 1358 bp and encoded proteins with mol wts from 29.5 to 40.3 kD. Five genes were grouped into three major clades based on amino acid sequences of encoded proteins. Exon-intron gene structure and protein domain analysis further supported the designation. A three-dimensional structure comparison showed the high similarity between five P. canadensis chitinases and three well-studied chitinases from other species. The expression levels of all five genes were up-regulated during Populus infection with the pathogenic fungus Marssonina brunnea, and four of them were highly induced by salt and drought stresses. Furthermore, such factors as elicitors, wounding, and low temperature also elevated the expression of these chitinase genes to varying extents. We postulated that these chitinase genes may be involved in pathways of the defense against fungal infection and function in response to various abiotic stresses.

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Abbreviations

CTAB:

cetyltrimethylammonium bromide

RACE:

rapid amplification of cDNA ends

RT-PCR:

reverse transcription-polymerase chain reaction

References

  1. Cohen-Kupiec, R. and Chet, I., The Molecular Biology of Chitin Digestion, Curr. Opin. Biotechnol., 1998, vol. 9, pp. 270–277.

    Article  PubMed  CAS  Google Scholar 

  2. Kasprzewska, A., Plant Chitinases — Regulation and Function, Cell Mol. Biol. Lett., 2003, vol. 8, pp. 809–824.

    PubMed  CAS  Google Scholar 

  3. Sharma, N., Sharma, K., Gaur, R., and Gupta, V., Role of Chitinase in Plant Defense, Asian. J. Biochem., 2011, vol. 6, pp. 29–37.

    Article  CAS  Google Scholar 

  4. Ubhayasekera, W., Structure and Function of Chitinases from Glycoside Hydrolase Family 19, Polymer Int., 2011, vol. 60, pp. 890–896.

    Article  CAS  Google Scholar 

  5. Shimono, K., Matsuda, H., and Kawamukai, M., Functional Expression of Chitinase and Chitosanase, and Their Effects on Morphologies in the Yeast Schizosaccharomyces pombe, BioSci. Biotechnol. Biochem., 2002, vol. 66, pp. 1143–1147.

    Article  PubMed  CAS  Google Scholar 

  6. Zhang, J., Zhang, X., Arakane, Y., Muthukrishnan, S., Kramer, K.J., Ma, E., and Zhu, K.Y., Comparative Genomic Analysis of Chitinase and Chitinase-Like Genes in the African Malaria Mosquito (Anopheles gambiae), PLoS One, 2011, vol. 6, p. e19899.

    Google Scholar 

  7. Santos, P., Fortunato, A., Ribeiro, A., and Pawlowski, K., Chitinases in Root Nodules, Plant Biotechnol., 2008, vol. 25, pp. 299–307.

    Article  CAS  Google Scholar 

  8. Passarinho, P.A. and de Vries, S.C., Arabidopsis Chitinases: A Genomic Survey, The Arabidopsis Book, 2002, pp. 1–25.

    Google Scholar 

  9. Kim, S.H., Choi, H.S., Cho, Y.C., and Kim, S.R., Cold-Responsive Regulation of a Flower-Preferential Class III Peroxidase Gene, ospox1, in Rice (Oryza sativa L.), J. Plant Biol., 2012, vol. 55, pp. 123–131.

    Article  CAS  Google Scholar 

  10. Brameld, K.A. and Goddard, W.A., The Role of Enzyme Distortion in the Single Displacement Mechanism of Family 19 Chitinases, Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 4276–4281.

    Article  PubMed  CAS  Google Scholar 

  11. Van Aalten, D., Komander, D., Synstad, B., Gåseidnes, S., Peter, M., and Eijsink, V., Structural Insights into the Catalytic Mechanism of a Family 18 Exo-Chitin, Proc. Natl. Acad. Sci. USA,2001, vol. 98, pp. 8979–8984.

    Article  PubMed  Google Scholar 

  12. Hamel, F., Boivin, R., Tremblay, C., and Bellemare, G., Structural and Evolutionary Relationships among Chitinases of Flowering Plants, J. Mol. Evol., 1997, vol. 44, pp. 614–624.

    Article  PubMed  CAS  Google Scholar 

  13. Graham, L.S. and Sticklen, M.B., Plant Chitinases, Can. J. Bot., 1994, vol. 72, pp. 1057–1083.

    Article  CAS  Google Scholar 

  14. Nandakumar, R., Babu, S., Kalpana, K., Raguchander, T., Balasubramanian, P., and Samiyappan, R., Agrobacterium-Mediated Transformation of Indica Rice with Chitinase Gene for Enhanced Sheath Blight Resistance, Biol. Plant., 2007, vol. 51, pp. 142–148.

    Article  CAS  Google Scholar 

  15. Salami, S., Ebadi, A., Naghavi, M., and Dry, I., Cloning and Functional Characterization of a Class III Chitinase Gene from Grapevine: Inhibition of Fungal Growth by Recombinant VvChiF III, Afr. J. Biotechnol., 2008, vol. 7, pp. 4400–4405.

    CAS  Google Scholar 

  16. Naher, L., Ho, C.L., Tan, S.G., Yusuf, U.K., and Abdullah, F., Cloning of Transcripts Encoding Chitinases from Elaeis guineensis Jacq. and Their Expression Profiles in Response to Fungal Infections, Physiol. Mol. Plant Pathol., 2011, vol. 76, pp. 96–103.

    Article  CAS  Google Scholar 

  17. Tuskan, G.A., Difazio, S., Jansson, S., Bohlmann, J., Grigoriev, I., Hellsten, U., Putnam, N., Ralph, S., Rombauts, S., Salamov, A., Schein, J., Sterck, L., Aerts, A., Bhalerao, R.R., Bhalerao, R.P., Blaudez, D., Boerjan, W., Brun, A., Brunner, A., Busov, V., Campbell, M., Carlson, J., Chalot, M., Chapman, J., Chen, G.L., Cooper, D., Coutinho, P.M., Couturier, J., Covert, S., Cronk, Q., Cunningham, R., Davis, J., Degroeve, S., Dejardin, A., Depamphilis, C., Detter, J., Dirks, B., Dubchak, I., Duplessis, S., Ehlting, J., Ellis, B., Gendler, K., Goodstein, D., Gribskov, M., Grimwood, J., Groover, A., Gunter, L., Hamberger, B., Heinze, B., Helariutta, Y., Henrissat, B., Holligan, D., Holt, R., Huang, W., Islam-Faridi, N., Jones, S., Jones-Rhoades, M., Jorgensen, R., Joshi, C., Kangasjärvi, J., Karlsson, J., Kelleher, C., Kirkpatrick, R., Kirst, M., Kohler, A., Kalluri, U., Larimer, F., Leebens-Mack, J., Leplé, J.C., Locascio, P., Lou, Y., Lucas, S., Martin, F., Montanini, B., Napoli, C., Nelson, D.R., Nelson, C., Nieminen, K., Nilsson, O., Pereda, V., Peter, G., Philippe, R., Pilate, G., Poliakov, A., Razumovskaya, J., Richardson, P., Rinaldi, C., Ritland, K., Rouzé, P., Ryaboy, D., Schmutz, J., Schrader, J., Segerman, B., Shin, H., Siddiqui, A., Sterky, F., Terry, A., Tsai, C.J., Uberbacher, E., Unneberg, P., Vahala, J., Wall, K., Wessler, S., Yang, G., Yin, T., Douglas, C., Marra, M., Sandberg, G., van de Peer, Y., and Rokhsar, D., The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray), Science, 2006, vol. 313, pp. 1596–1604.

    Article  PubMed  CAS  Google Scholar 

  18. Chung, H.S., Koo, A.J., Gao, X., Jayanty, S., Thines, B., Jones, A.D., and Howe, G.A., Regulation and Function of Arabidopsis JASMONATE ZIM-Domain Genes in Response to Wounding and Herbivory, Plant Physiol., 2008, vol. 146, pp. 952–964.

    Article  PubMed  CAS  Google Scholar 

  19. Cheng, Q., Cao, Y., Jiang, C., Xu, L., Wang, M., Zhang, S., and Huang, M., Identifying Secreted Proteins of Marssonina brunnea by Degenerate PCR, Proteomics, 2010, vol. 10, pp. 2406–2417.

    Article  PubMed  CAS  Google Scholar 

  20. Xu, M., Zhang, B., Yao, H.S., and Huang, M.R., Isolation of High Quality RNA and Molecular Manipulations with Various Tissues of Populus, Russ. J. Plant Physiol., 2009, vol. 56, pp. 716–719.

    Article  CAS  Google Scholar 

  21. Zhang, Y., I-Tasser Server for Protein 3D Structure Prediction, BMC Bioinformatics, 2008, vol. 9, p. 40.

    Article  PubMed  Google Scholar 

  22. Roy, A., Kucukural, A., and Zhang, Y., I-Tasser: A Unified Platform for Automated Protein Structure and Function Prediction, Nat. Protoc., 2010, vol. 5, pp. 725–738.

    Article  PubMed  CAS  Google Scholar 

  23. Holm, L. and Park, J., Dalilite Workbench for Protein Structure Comparison, Bioinformatics, 2000, vol. 16, pp. 566–567.

    Article  PubMed  CAS  Google Scholar 

  24. Kezuka, Y., Kojima, M., Mizuno, R., Suzuki, K., Watanabe, T., and Nonaka, T., Structure of Full-Length Class I Chitinase from Rice Revealed by X-Ray Crystallography and Small-Angle X-Ray Scattering, Proteins, 2010, vol. 78, pp. 2295–2309.

    Article  PubMed  CAS  Google Scholar 

  25. Ohnuma, T., Numata, T., Osawa, T., Mizuhara, M., Lampela, O., Juffer, A.H., Skriver, K., and Fukamizo, T., A Class V Chitinase from Arabidopsis thaliana: Gene Responses, Enzymatic Properties, and Crystallographic Analysis, Planta, 2011, vol. 234, pp. 123–137.

    Article  PubMed  CAS  Google Scholar 

  26. Hennig, M., Jansonius, J.N., Terwisscha van Scheltinga, A.C., Dijkstra B.W., and Schlesier, B., Crystal Structure of Concanavalin B at 1.65 A Resolution. An “Inactivated” Chitinase from Seeds of Canavalia ensiformis, J. Mol. Biol., 1995, vol. 254, pp. 237–246.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing, 210037, China

    C. Jiang, J. Song, R. Huang, M. Huang & L. Xu

Authors
  1. C. Jiang
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  2. J. Song
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  3. R. Huang
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  4. M. Huang
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  5. L. Xu
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Correspondence to C. Jiang or L. Xu.

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This text was submitted by the authors in English.

These authors contributed equally to this work.

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Jiang, C., Song, J., Huang, R. et al. Cloning and expression analysis of Chitinase genes from Populus canadensis . Russ J Plant Physiol 60, 396–403 (2013). https://doi.org/10.1134/S1021443713030072

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  • DOI: https://doi.org/10.1134/S1021443713030072

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