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Heterologous expression of a halophilic archaeon manganese superoxide dismutase enhances salt tolerance in transgenic rice

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Abstract

In order to investigate new gene resource for enhancing rice tolerance to salt stress, manganese superoxide dismutase gene from halophilic archaeon (Natrinema altunense sp.) (NaMnSOD) was isolated and introduced into Oryza sativa L. cv. Nipponbare by Agrobacterium-mediated transformation. The transformants (L1 and L2) showed some NaMnSOD expression and increased total SOD and CAT activity, which contributed to higher efficiency of ROS elimination under salt stress. The levels of superoxide anion radicals (O ·−2 ) and hydrogen peroxide (H2O2) were significantly decreased. In addition, they exhibited higher levels of photosynthesis, whereas lower relative ion leakage and MDA content compared to wild-type plants. Therefore, transgenic seedlings were phenotypically healthier, and heterologous expression of NaMnSOD could improve rice salt tolerance.

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Abbreviations

CaMV:

cauliflower mosaic virus

CAT:

catalase

Chl:

chlorophyll

F 0 :

the minimum fluorescence yield

F m :

the maximum fluorescence yield

F s :

stable fluorescence yield

GUS:

β-glucuronidase

L1/L2:

transgenic lines

NBT:

nitroblue tetrazolium chloride

O ·−2 :

superoxide anion radical

PCR:

polymerase chain reaction

RT-PCR:

reverse transcription-polymerase chain reaction

SOD:

superoxide dismutase

WT:

wild type

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, P.R. China

    Z. Chen, L. Y. An, W. J. Yang, L. G. Xu & C. Zhu

  2. College of Life Sciences, China Jiliang University, Hangzhou, 310018, P.R. China

    Y. H. Pan & C. Zhu

  3. School of Life Sciences, Taizhou University, Taizhou, 317000, P.R. China

    Z. Chen

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  1. Z. Chen
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  2. Y. H. Pan
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  3. L. Y. An
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  4. W. J. Yang
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  5. L. G. Xu
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  6. C. Zhu
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Correspondence to Z. Chen.

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Chen, Z., Pan, Y.H., An, L.Y. et al. Heterologous expression of a halophilic archaeon manganese superoxide dismutase enhances salt tolerance in transgenic rice. Russ J Plant Physiol 60, 359–366 (2013). https://doi.org/10.1134/S1021443713030059

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