Abstract
The seedlings of six cultivars of oil-seed flax (Linum humile Mill.) differing in the extent of adaptation to abiotic stresses (hypo- and hyperthermia, osmotic stress, and salinity) were used to assess hemag-glutination activity and carbohydrate specificity of total lectin preparations extracted from various cell compartments. In the course of adaptation of plants resistant to hyperthermia, osmotic stress and salinity, we observed a considerable rise in the coefficient of activity of membrane lectins, whereas the adaptation to hypothermia elevated the coefficient of activity of cell wall lectins. As to total soluble lectins, the adaptation of flax plants was associated with the changes in the range of their carbohydrate specificity. For instance, following the adaptation to hyperthermia, they were found to bind glucose and glucosamine, to osmotic stress—mannose and xylose, to salinity—galactose, glucose, and glucosamine; after cold resistance was developed, total soluble lectins were found to recognize lactose and fructose. It was concluded that lectins may participate in specific adaptation of flax plants to various abiotic stress factors.
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Abbreviations
- LA:
-
lectin activity.
References
Kletochnye mekhanizmy adaptatsii rastenii k neblagopriyatnym vozdeistviyam ekologicheskikh faktorov v estestvennykh usloviyakh (Cellular Mechanisms for Plant Adaptation to Environmental Ecological Factors), Kordyum, E.L., Ed., Kiev: Nauk. Dumka, 2003.
Kolupaev, Yu.E. and Karpets, Yu.V., Formirovanie adaptivnykh reaktsii rastenii na deistvie abioticheskikh stressorov (Development of Adaptive Plant Responses to Abiotic Stressors), Kiev: Osnova, 2010.
Jiang, S., Ma, Z., and Ramachandran, S., Evolutionary History and Stress Regulation of the Lectin Superfamily in Higher Plants, BMC Evol. Biol., 2010, vol. 10, pp. 79–103.
Lutsik, M.D., Panasyuk, V.M., and Lutsik, A.D., Lektiny (Lectins), Lviv: Vishcha Shkola, 1981.
Antonyuk, V.O., Lektini ta ikh sirovinni dzherela (Lectins and Their Origin). Lviv: PP “Kvart”, 2005.
Shakirova, F.M., Nespetsificheskaya ustoichivost’ rastenii k stressovym faktoram i ee regulyatsiya (Nonspecific Plant Resistance to Stress Factors and Its Regulation), Ufa: Gilem, 2001.
Babosha, A.V., Inducible Lectins and Plant Resistance to Pathogens and Abiotic Stress, Russ. J. Biochem., 2008, vol. 73, pp. 812–827.
Timofeeva, O.A., Khokhlova, L.P., Trifonova, T.V., Belyaeva, N.E., and Chulkova, Yu.Yu., Effect of Cytoskeleton Modifiers on the Lectin Activity during Plant Acclimation to Low Temperatures and under ABA Treatment, Russ. J. Plant. Physiol., 1999, vol. 46, pp. 147–151.
Komarova, E.M., Vyskrebentseva, E.I., and Trunova, T.I., Activity of Lectin-Like Proteins of the Cell Walls and the Outer Organelle Membranes as Related to Endogenous Ligands in Cold-Adapted Seedlings of Winter Wheat, Russ. J. Plant Physiol., 2003, vol. 50, pp. 455–459.
Cammue, B.P.A., Broecaert, W.F., Kellens, J.T.S., Raikhel, N.V., and Peumans, V.I., Stress-Induced Accumulation of Wheat Germ Agglutinin and Abscisic Acid in Roots of Wheat Seedlings, Plant Physiol., 1989, vol. 91, pp. 1432–1435.
Shakirova, F.M., Bezrukova, M.V., Aval’baev, A.M., and Fatkhutdinova, R.A., Control Mechanisms of Lectin Accumulation in Wheat Seedlings under Salinity, Russ. J. Plant Physiol., 2003, vol. 50, pp. 301–304.
Kil’dibekova, A.R., Bezrukova, M.V., Aval’baev, A.M., Fatkhutdinova, R.A., and Shakirova, F.M., Mechanisms of Wheat Embryo Lectin Protective Effect on Root Cell Growth of Wheat Seedlings under Salinity, Tsitologiya, 2004, vol. 46, pp. 312–315.
Bezrukova, M., Kildibecova, A., and Shakirova, F., WGA Reduces the Level of Oxidative Stress in Wheat Seedlings, Plant Growth Regul., 2008, vol. 54, pp. 195–201.
Joshi, A., Dang, H.Q., Vaid, N., and Tuteja, N., Pea Lectin Receptor-Like Kinase Promoters High Salinity Stress Tolerance in Bacteria and Expresses in Response to Stress In Planta, Glycoconjug. J., 2010, vol. 27, pp. 133–150.
Levchuk, G.M. and Voitovich, O.M., Linum Lectin as the Factor of Adaptation to Extremal Temperatures, Naukovi, prikladni ta osvitni aspekti fiziologiï, genetiki, biotekhnologiï roslin i mikroorganizmiv: Mater. XI Konf. Molod. Vchenikh (Proc. XI Conf. Young Scientists Scientific and Applied, Aspects of Plant and Microorganism Physiology, Genetics, and Biotechnology), Kiev, 2010, pp. 96–98.
Komarova, E.M., Vyskrebentseva, E.I., and Trunova, T.I., Distribution of Lectin Activity in Mitochondria of Winter Wheat Crowns during Hardening, Russ. J. Plant Physiol., 1995, vol. 42, pp. 543–546.
Dawson, R., Elliot, D., Elliot, U., and Jones, K., Data for Biochemical Research, Oxford: Clarendon, 1986.
Lakin, G.F., Biometriya (Biometrics), Moscow: Vysshaya Shkola, 1990.
Komarova, E.M., Vyskrebentseva, E.I., and Trunova, T.I., Distribution of Lectin Activity in Cell Walls of Winter Wheat Etiolated Seedlings during Cold Hardening, Dokl. Ross. Akad. Nauk, 1993, vol. 329, pp. 680–682.
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Original Russian Text © A.N. Levchuk, E.N. Voitovich, V.A. Lyakh, 2013, published in Fiziologiya Rastenii, 2013, Vol. 60, No. 1, pp. 75–81.
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Levchuk, A.N., Voitovich, E.N. & Lyakh, V.A. Lectins of oil-seed flax plants exposed to abiotic stress. Russ J Plant Physiol 60, 77–83 (2013). https://doi.org/10.1134/S1021443712060106
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DOI: https://doi.org/10.1134/S1021443712060106