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Comparative study on calli from two reed ecotypes under heat stress

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Abstract

The different physiological responses to heat stress in calli from two ecotypes of common reed (Phragmites communis Trin.) plants (dune reed (DR) and swamp reed (SR)) were studied. The relative water content, the relative growth rate, cell viability, membrane permeability (MP), H2O2 content, MDA content, proline level, and the activities of enzymes, such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR), and lipoxygenase (LOX) were assayed. Results showed that under heat stress, DR callus could maintain the higher relative growth rate and cell viability than SR callus, while H2O2 content, MDA content, and MP in SR callus increased more than in DR callus. The activities of antioxidant enzymes, such as SOD, CAT, POD, APX, and GR in two calli were enhanced by high temperature. However, antioxidant enzymes in DR callus showed the higher thermal stability than those in SR callus. LOX activity increased more in SR callus than in DR callus under heat stress. High temperature markedly elevated proline content in DR callus whereas had no effect on that in SR callus. Taken together, DR callus is more thermotolerant than SR callus, which might be due to the higher activity of antioxidant enzymes and proline level compared with SR callus under heat stress.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

DR:

dune reed

GR:

glutathione reductase

LOX:

lipoxygenase

MP:

membrane permeability

NBT:

nitro blue tetrazolium

POD:

peroxidase

PVP:

polyvinylpyrrolidone

RGR:

relative growth rate

RWC:

relative water contents

SR:

swamp reed

SOD:

superoxide dismutase

TTC:

2,3,5-triphenyl tetrazolium chloride

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Authors and Affiliations

  1. School of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai, 200235, P.R. China

    L. Song, H. Zhao & Zh. Zhang

  2. State-Owned Miaoshou Forest Farm, Jingde Anhui, 242604, P.R. China

    Y. Jiang

Authors
  1. L. Song
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  2. Y. Jiang
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  3. H. Zhao
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  4. Zh. Zhang
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Correspondence to L. Song.

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The text was submitted by the authors in English.

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Song, L., Jiang, Y., Zhao, H. et al. Comparative study on calli from two reed ecotypes under heat stress. Russ J Plant Physiol 59, 381–388 (2012). https://doi.org/10.1134/S1021443712030168

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  • DOI: https://doi.org/10.1134/S1021443712030168

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