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Antioxidant activity in the leaves of Melilotus albus and Trifolium medium from man-made disturbed habitats in the Middle Urals under the influence of copper

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Abstract

Physiological mechanisms of adaptation to copper-induced stress in two widespread legume plants, white sweet clover (Melilotus albus Merik.) and zigzag clover (Trifolium medium L.), growing in habitats differing in the man-made pollution. An antioxidant plant defense system was activated in response to 10 mM CuSO4, which is a stress factor. Specific biochemical features related to adaptation to soil contamination with copper were observed in tested plant species. Superoxide dismutase was activated in response to stress in both species from various habitats. M. albus from the impact zone manifested the better capacity of proline accumulation as compared with plants from less polluted habitats. T. medium plants from the impact zone contained more active peroxidase. It was suggested that plants growing for a long time under stressful conditions manifest the greater tolerance to copper ions than plants, which did not experience stress or were subjected to the milder stress.

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Abbreviations

POL:

peroxidation of lipids

SOD:

superoxide dismutase

References

  1. Erofeeva, E.A. and Naumova, M.M., Intensity of Lipid Peroxidation in the Leaf Blade of Betula pendula from Biotopes with Different Levels of Anthropogenic Load, Vestn. Nizhegorod. Gos. Univ. im. N.I. Lobachevskogo, 2008, no. 3, pp. 87–91.

  2. Weber, H., Chetelat, A., Reymond, P., and Farmer, E., Selective and Powerful Stress Gene Expression in Arabidopsis in Response to Malondialdehyde, Plant J., 2004, vol. 37, pp. 877–888.

    Article  PubMed  CAS  Google Scholar 

  3. Kuznetsov, Vl.V. and Shevyakova, N.I., Proline under Stress: Biological Role, Metabolism, and Regulation, Russ. J. Plant Physiol., 1999, vol. 46, pp. 274–288.

    CAS  Google Scholar 

  4. Veselov, A.P., Chumankina, E.A., and Markina, I.V., Effect of Exogenous Hydrogen Peroxide on Lipid Peroxidation and Enzymes of Antioxidant Defense during Wild Plant Adaptation to Salt Stress, Vestn. Nizhegorod. Gos. Univ. im. N.I. Lobachevskogo, Ser. Biologiya, 2001, pp. 164–167.

  5. Kartashov, A.V., Radyukina, N.L., Ivanov, Yu.V., Pashkovskii, P.P., Shevyakova, N.I., and Kuznetsov, Vl.V., Role of Antioxidant Systems in Wild Plant Adaptation to Salt Stress, Russ. J. Plant Physiol., 2008, vol. 55, pp. 463–468.

    Article  CAS  Google Scholar 

  6. Merzlyak, M.N., Activated Oxygen and Plant Activity, Sorosov. Obrazov. Zhurn., 1999, no. 9, pp. 20–26.

  7. Boyarkin, D.V., Gubanov, L.N., and Fedorovskii, D.N., Neutralization of Sludge from Municipal Wastewaters Using Legume Plants, Voda i Ekologiya: Problemy i Resheniya, 2003, no. 4, pp. 55–58.

  8. Savinov, A.B., Kurganova, L.N., and Shekunov, Yu.I., Lipid Peroxidation Rates in Taraxacum officinale Wigg. and Vicia cracca L. from Biotopes with Different Levels of Soil Pollution with Heavy Metals, Ekologiya (Yekaterinburg), 2007, no. 3, pp. 191–195.

  9. Prasad, M.N.V., Trace Elements as Contaminants and Nutrients. Consequences in Ecosystems and Human Health, Chichester: Wiley, 2008.

    Book  Google Scholar 

  10. Opredelitel’ sosudistykh rastenii Srednego Urala (Vascular Plants of the Middle Urals), Gorchakovskii, P.L., Shurova, E.A., Knyazev, M.S., et al., Eds., Moscow: Nauka, 1994.

    Google Scholar 

  11. Zhuikova, T.V., Bezel’, V.S., and Mordvina, E.S., Phytocenosis of Technogeneously Disturbed Areas and Their Role in the Biogenic Cycles of Chemical Elements, Uch. zapiski Nizhnetagil’skoi gosudarstvennoi sotsial’no-pedagogicheskoi akademii (Scientific Proceeding of Nizhnetagil. Gos. Soc.-Ped. Akad.), Zhuikova, T.V., Ed., Nizhnii Tagil: Nizhnetagil. Gos. Soc.-Ped. Akad., 2006, pp. 31–72.

    Google Scholar 

  12. Bates, L.S., Rapid Determination of Free Proline for Stress Studies, Plant Soil, 1973, vol. 39, pp. 205–207.

    Article  CAS  Google Scholar 

  13. Kalinkina, L.G., Nazarenko, L.V., and Gordeeva, E.E., Modified Method for Extraction of Free Amino Acids and Their Determination in Amino Acid Analyzer, Sov. Plant Physiol., 1990, vol. 37, pp. 617–621.

    CAS  Google Scholar 

  14. Metody otsenki ustoichivosti rastenii k stressovym faktoram. Rukovodstvo dlya bol’shogo spetsial’nogo praktikuma po fiziologii i biokhimii rastenii (Methods for the Assessment of Plant Tolerance to Stresses. Special Manual for Plant Physiologists and Biochemists), Nekrasova, G.F., Maleva, M.G., Eds., Yekaterinburg: Ural’sk. Gos. Univ. im. A.M. Gor’kogo, 2007.

    Google Scholar 

  15. Beauchamp, C. and Fridovich, I., Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels, Anal. Biochem., 1971, vol. 44, pp. 276–287.

    Article  PubMed  CAS  Google Scholar 

  16. Devi, S.R. and Prasad, M.N.V., Antioxidant Capacity of Brassica juncea Plants Exposed to Elevated Levels of Copper, Russ. J. Plant Physiol., 2005, vol. 52, pp. 205–208.

    Article  CAS  Google Scholar 

  17. Kholodova, V.P., Volkov, K.S., and Kuznetsov, Vl.V., Adaptation of the Common Ice Plant to High Copper and Zinc Concentrations and Their Potential Using for Phytoremediation, Russ. J. Plant Physiol., 2005, vol. 52, pp. 748–757.

    Article  CAS  Google Scholar 

  18. Shusharin, M.M. and Zhuravskaya, A.N., Investigation of Plant Adaptive Resources in Zones with Anthropogenic Impact, Ekologiya (Yekaterinburg), 2007, no. 2, pp. 93–98.

  19. Luna, C.M., Gonzalez, C.A., and Trippi, V.S., Oxidative Damage Caused by an Excess of Copper in Oat Leaves, Plant Cell Physiol., 1994, vol. 35, pp. 11–15.

    CAS  Google Scholar 

  20. Kurganova, L.N., Lipid Peroxidation Is One of Possible Components of Plant Rapid Response to Stress, Mater. vyezdnoi sessii OFR RAN po problemam bioelektrogeneza i adaptatsii u rastenii, Vestn. Nizhegorod. un-ta im. N.M. Lobachevskogo. Ser. Biologiya (Proc. Soc. Plant Physiol. Ross. Akad. Nauk, Vest. Nizhegorod. Gos. Univ. im. N.M. Lobachevskogo, Ser. Biology), Nizhnii Novgorod: Nizhegorod. Gos. Univ., 2001, pp. 76–78.

    Google Scholar 

  21. Gamalei, I.A. and Klyubin, I.V., Hydrogen Peroxide as a Signal Molecule, Tsitologiya, 1996, vol. 38, pp. 1233–1247.

    CAS  Google Scholar 

  22. Maksyutova, N.N., Viktorova, L.V., Trifonova, T.V., and Galeeva, I.E., Hydrogen Peroxide Formation and Activity of Extracellular Peroxidases during Injection of Salicylic Acid into Wheat Leaf Apoplast, Vestn. Kharkov. Nats. Univ. im. V.N. Karazina, Ser. Biologiya, 2009, Iss. 10, no. 878, pp. 28–33.

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Authors and Affiliations

  1. Gorkii Ural State University, pr. Lenina 51, Yekaterinburg, 620083, Russia

    E. R. Fazlieva & I. S. Kiseleva

  2. Nizhnii Tagil State Social and Pedagogical Academy, Nizhnii Tagil, Russia

    T. V. Zhuikova

Authors
  1. E. R. Fazlieva
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  2. I. S. Kiseleva
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  3. T. V. Zhuikova
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Correspondence to I. S. Kiseleva.

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Original Russian Text © E.R. Fazlieva, I.S. Kiseleva, T.V. Zhuikova, 2012, published in Fiziologiya Rastenii, 2012, Vol. 59, No. 3, pp. 369–375.

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Fazlieva, E.R., Kiseleva, I.S. & Zhuikova, T.V. Antioxidant activity in the leaves of Melilotus albus and Trifolium medium from man-made disturbed habitats in the Middle Urals under the influence of copper. Russ J Plant Physiol 59, 333–338 (2012). https://doi.org/10.1134/S1021443712030065

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