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Effect of leghemoglobin A gene expression from soybean on tobacco plant growth and antioxidant state under damaging action of cadmium

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Abstract

Transgenic tobacco (Nicotiana tabacum L. plants, cv. Samsun) bearing the gene for soybean (Glycine max (L.) Merr.) leghemoglobin A under the control of 35S CaMV promoter were produced. The effects of this gene expression on tobacco growth and respiration, MDA content, and also activities of catalase and guaicol peroxidase were studied. The growth rate of transformed plant was reduced, respiratory losses were increased, and lipid peroxidation was substantially suppressed. In plants expressing the laghemoglobin A gene, the negative effects of toxic cadmium concentrations on growth parameters and plant oxidative status were weakened.

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Abbreviations

AO:

alternative oxidase

Hg:

hemoglobin

Lg:

leghemoglobin

MS:

Murashige and Skoog nutrient medium

P g :

gross photosynthesis

POL:

peroxidation of lipids

R a :

adaptation respiration

RGR:

relative growth rate

ΣR :

total respiration

W:

plant dry weight

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Authors and Affiliations

  1. Department of Plant Physiology, Faculty of Biology, Bashkir State University, ul. Zaki Validi 32, Ufa, 450007, Russia

    M. Yu. Dmitryukova, V. V. Fedyaev & Z. F. Rakhmankulova

  2. Institute of Biochemistry and Genetics, Russian Academy of Sciences, Ufa Research Center, Ufa, Russia

    A. Kh. Baimiev

Authors
  1. M. Yu. Dmitryukova
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  2. A. Kh. Baimiev
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  3. V. V. Fedyaev
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  4. Z. F. Rakhmankulova
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Correspondence to M. Yu. Dmitryukova.

Additional information

Original Russian Text © M.Yu. Dmitryukova, A.Kh. Baimiev, V.V. Fedyaev, Z.F. Rakhmankulova, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 6, pp. 915–921.

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Dmitryukova, M.Y., Baimiev, A.K., Fedyaev, V.V. et al. Effect of leghemoglobin A gene expression from soybean on tobacco plant growth and antioxidant state under damaging action of cadmium. Russ J Plant Physiol 58, 1055–1061 (2011). https://doi.org/10.1134/S1021443711060057

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  • DOI: https://doi.org/10.1134/S1021443711060057

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