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Stress responses of wheat leaves to dehydration: Participation of endogenous NO and effect of sodium nitroprusside

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Abstract

Dynamics of endogenous NO in the leaves of 7-day-old seedlings of spring wheat (Triticum aestivum L., cv. Debyut) and the effect of exogenous NO donor (sodium nitroprusside, SNP) on the development of oxidative stress and activity of antioxidant enzymes in the leaves under water deficiency were investigated. Quick and phasic accumulation of NO in the leaves was observed under growing dehydration (0–3 h) and subsequent rehydration (0–3 h), which points to identical response of NO signal system to opposite changes in the water status of plants. A decrease in relative turgidity of tissues brought about accumulation of H2O2 and MDA therein. Protective effect of NO donor infiltrated in the leaves was associated with an elevation of ascorbate peroxidase and catalase activities and suppression of lipid peroxidation upon dehydration. Pretreatment with SNP (50–250 μM) induced the elevation of NO level in the leaves both before action of the stress agent and in the beginning (0–30 min) of dehydration. The obtained results suggest that brief increase in endogenous NO is necessary for triggering protective and adaptive responses in wheat plants during the development of water deficiency.

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Abbreviations

APO:

ascorbate peroxidase

CAT:

catalase

POL:

peroxidation of lipids

RWC:

relative water content

SNP:

sodium nitroprusside

References

  1. Neill, S., Barros, R., Bright, J., Desikan, R., Hancock, J., Harrison, J., Morris, P., Ribeiro, D., and Wilson, I., Nitric Oxide, Stomatal Closure and Abiotic Stress, J. Exp. Bot., 2008, vol. 59, pp. 165–176.

    Article  PubMed  CAS  Google Scholar 

  2. Zhao, L., Zhang, F., and Guo, J., Nitric Oxide Functions as a Signal in Salt Resistance in the Calluses from Two Ecotypes of Reed, Plant Physiol., 2004, vol. 134, pp. 849–857.

    Article  PubMed  CAS  Google Scholar 

  3. Zhang, H., Li, Y.H., Hu, L.Y., Wang, S.H., Zhang, F.Q., and Hu, K.D., Effects of Exogenous Nitric Oxide Donor on Antioxidant Metabolism in Wheat Leaves under Aluminum Stress, Russ. J. Plant Physiol., 2008, vol. 55, pp. 469–474.

    Article  CAS  Google Scholar 

  4. Zhao, M., Chen, L., Zhang, L., and Zhang, W., Nitric Reductase-Dependent Nitric Oxide Production Is Involved in Cold Acclimation and Freezing Tolerance in Arabidopsis, Plant Physiol., 2009, vol. 151, pp. 755–767.

    Article  PubMed  CAS  Google Scholar 

  5. Uchida, A., Jagendorf, A., Hibino, T., and Takabe, T., Effects of Hydrogen Peroxide and Nitric Oxide on Both Salt and Heat Stress Tolerance in Rice, Plant Sci., 2002, vol. 163, pp. 515–523.

    Article  CAS  Google Scholar 

  6. Tyan, S.R. and Lei, Yu.B., Physiological Responses of Wheat Seedlings to Drought and UV-B Radiation. Effect of Exogenous Sodium Nitroprusside Application, Russ. J. Plant Physiol., 2007, vol. 54, pp. 676–682.

    Article  Google Scholar 

  7. Passioura, J., The Drought Environment: Physical, Biological and Agricultural Perspectives, J. Exp. Bot., 2007, vol. 58, pp. 113–117.

    Article  PubMed  CAS  Google Scholar 

  8. García-Mata, C. and Lamattina, L., Nitric Oxide Induces Stomatal Closure and Enhances the Adaptive Plant Responses against Drought Stress, Plant Physiol., 2001, vol. 126, pp. 1196–1204.

    Article  PubMed  Google Scholar 

  9. Zhou, B., Guo, Z., Xing, J., and Huang, B., Nitric Oxide Is Involved in Abscisic Acid-Induced Antioxidant Activities In Stylosanthes guianensis, J. Exp. Bot., 2005, vol. 56, pp. 3223–3228.

    Article  CAS  Google Scholar 

  10. Zhang, A., Jiang, M., Zhang, J., Ding, H., Xu, S., Hu, X., and Tan, M., Nitric Oxide Induced by Hydrogen Peroxide Mediates Abscisic Acid-Induced Activation of the Mitogen-Activated Protein Kinase Cascade Involved in Antioxidant Defense in Maize Leaves, New Phytol., 2007, vol. 175, pp. 36–50.

    Article  PubMed  CAS  Google Scholar 

  11. Sang, J., Jiang, M., Lin, F., Xu, S., Zhang, A., and Tan, M., Nitric Oxide Reduced Hydrogen Peroxide Accumulation Involved in Water Stress-Induced Subcellular Antioxidant Defense in Maize Plants, J. Integr. Plant Biol., 2008, vol. 50, pp. 231–243.

    Article  PubMed  CAS  Google Scholar 

  12. Xu, M.-J., Dong, J.-F., and Zhu, M.-Y., Nitric Oxide Mediates the Fungal Elicitor-Induced Hypericin Production of Hypericum perforatum Cell Suspension Cultures through a Jasmonic-Acid-Dependent Signal Pathway, Plant Physiol., 2005, vol. 139, pp. 991–998.

    Article  PubMed  CAS  Google Scholar 

  13. Gay, C. and Gebicki, J.M., A Critical Evaluation of the Effect of Sorbitol on the Ferric-Xylenol Orange Hydroperoxide Assay, Anal. Biochem., 2000, vol. 284, pp. 217–220.

    Article  PubMed  CAS  Google Scholar 

  14. Heath, R.L. and Packer, L., Photoperoxidation in Isolated Chloroplasts. 1. Kinetics and Stoichiometry of Fatty Acid Peroxidation, Arch. Biochem. Biophys., 1968, vol. 125, pp. 189–198.

    Article  PubMed  CAS  Google Scholar 

  15. Tekhnika biokhimicheskogo issledovaniya subkletochnykh struktur i biopolimerov (Technique for Biochemical Investigations of Subcellular Structures and Biopolymers), Minsk: Nauka i Tekhnika, 1977.

  16. Nacano, Y. and Asada, K., Hydrogen Peroxide Is Scavenged by Ascorbate Specific Peroxidase in Spinach Chloroplasts, Plant Cell Physiol., 1981, vol. 22, pp. 867–880.

    Google Scholar 

  17. Beers, R.F. and Sizer, J.F., Colorimetric Method for Estimation of Catalase, J. Biol. Chem., 1953, vol. 195, pp. 133–139.

    Google Scholar 

  18. Kholodova, V.P., Bormotova, T.S., Semenov, O.G., Dmitrieva, G.A., and Kuznetsov, Vl.V., Physiological Mechanisms of Adaptation of Alloplasmic Wheat Hybrids to Soil Drought, Russ. J. Plant Physiol., 2007, vol. 54, pp. 480–486.

    Article  CAS  Google Scholar 

  19. Gould, K., Lamotte, O., Klinguer, A., Pugin, A., and Wendehenne, D., Nitric Oxide Production in Tobacco Leaf Cells: A Generalized Stress Response? Plant Cell Environ., 2003, vol. 26, pp. 1851–1862.

    Article  CAS  Google Scholar 

  20. Bright, J., Desikan, R., Hancock, J., Weir, I., and Neill, S., ABA-Induced NO Generation and Stomatal Closure in Arabidopsis Are Dependent on H2O2 Synthesis, Plant J., 2006, vol. 45, pp. 113–122.

    Article  PubMed  CAS  Google Scholar 

  21. Chirkova, T.V., Fiziologicheskie osnovy ustoichivosti rastenii (Physiological Basics of Plant Resistance), St. Petersburg: St. Petersburg Gos. Univ., 2002.

    Google Scholar 

  22. Dhindsa, R., Drought Stress, Enzymes of Glutathione Metabolism, Oxidation Injury, and Protein Synthesis in Tortula ruralis, Plant Physiol., 1991, vol. 95, pp. 648–651.

    Article  CAS  Google Scholar 

  23. Song, L., Ding, W., Zhao, M., Sun, B., and Zhang, L., Nitric Oxide Protects against Oxidative Stress under Heat Stress in the Calluses from Two Ecotypes of Reed, Plant Sci., 2006, vol. 171, pp. 449–458.

    Article  CAS  Google Scholar 

  24. Hu, X., Neill, S., Tang, Z., and Cai, W., Nitric Oxide Mediates Gravitropic Bending in Soybean Roots, Plant Physiol., 2005, vol. 137, pp. 663–670.

    Article  PubMed  CAS  Google Scholar 

  25. Jasid, S., Simontacchi, M., Bartoli, C., and Puntarulo, S., Chloroplasts as a Nitric Oxide Cellular Source. Effect of Reactive Nitrogen Species on Chloroplastic Lipids and Proteins, Plant Physiol., 2006, vol. 142, pp. 1246–1255.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology and Soil Science, Kazan Federal University, Kremlevskaya ul. 18, Kazan, 420008, Russia

    A. V. Boyarshinov & E. V. Asafova

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  1. A. V. Boyarshinov
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  2. E. V. Asafova
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Correspondence to A. V. Boyarshinov.

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Original Russian Text © A.V. Boyarshinov, E.V. Asafova, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 6, pp. 891–897.

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Boyarshinov, A.V., Asafova, E.V. Stress responses of wheat leaves to dehydration: Participation of endogenous NO and effect of sodium nitroprusside. Russ J Plant Physiol 58, 1034–1039 (2011). https://doi.org/10.1134/S1021443711060033

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