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Processes hindering activation of lipid peroxidation in cold-tolerant plants under hypothermia

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Abstract

The reasons why the rate of lipid peroxidation (POL) associated with a long-term action of low above-zero temperature (5°C, 6 days) on 6-week-old plants of two potato (Solanum tuberosum L.) cultivars (cold-tolerant cv. Desnitsa and less tolerant cv. Desiree) did not rise were investigated. Upon a long-term action of low hardening temperatures on the plants of both cultivars, there was an equilibrium between the rate of generation of superoxide anion (O ·−2 and activity of superoxide dismutase (SOD), which inactivated it with the formation of H2O2. Among the enzymes breaking up hydrogen peroxide, the highest activity was observed for guaiacol peroxidases, which was an order of magnitude greater than the activity of catalase. In potato cultivars, POL processes were not considerably activated; however, activities of antioxidant enzymes (SOD, catalase, and guaiacol peroxidases) in cold-tolerant cv. Desnitsa and less tolerant cv. Desiree differed. It was concluded that, upon a long-term action of hardening temperatures, cold-tolerant plants could sup-press POL processes. Moreover, a test for tolerance to damaging temperature (−3°C, 18 h) showed that detected preservation of the prooxidant/antioxidant equilibrium not only maintained vital activities at low above-zero temperatures but also elevated tolerance to short-term frosts, with this adaptability being cultivar-specific.

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Abbreviations

POL:

peroxidation of lipids

PMSF:

phenylme-thylsulfonyl fluoride

ROS:

reactive oxygen species

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    M. S. Sin’kevich, N. V. Naraikina & T. I. Trunova

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  1. M. S. Sin’kevich
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  2. N. V. Naraikina
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  3. T. I. Trunova
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Correspondence to M. S. Sin’kevich.

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Original Russian Text © M.S. Sin’kevich, N.V. Naraikina, T.I. Trunova, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 6, pp. 875–882.

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Sin’kevich, M.S., Naraikina, N.V. & Trunova, T.I. Processes hindering activation of lipid peroxidation in cold-tolerant plants under hypothermia. Russ J Plant Physiol 58, 1020–1026 (2011). https://doi.org/10.1134/S1021443711050232

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