Advertisement

Protein quantification

  • N. S. Stepanchenko
  • G. V. NovikovaEmail author
  • I. E. Moshkov
Lecture

Abstract

Protein quantification is an integral part of any investigation related to protein isolation, purification, characterization, and analysis. Although the methods considered in this lecture have multi-year history and applied widely in the laboratory practice, there are some crucial points, which must be taken into consideration while choosing the method permitting reliably and with a high specificity and reproducibility to quantify protein.

Keywords

plant protein quantification Lowry method Bradford method BCA reagent 

Abbreviations

BCA

bicinchoninic acid

References

  1. 1.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, pp. 265–275.PubMedGoogle Scholar
  2. 2.
    Bradford, M.M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.PubMedCrossRefGoogle Scholar
  3. 3.
    Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., and Klenk, D.C., Measurement of Protein Using Bicinchoninic Acid, Anal. Biochem., 1985, vol. 150, pp. 76–85.PubMedCrossRefGoogle Scholar
  4. 4.
    Gornall, A.G., Bardawill, C.S., and David, M.M., Determination of Serum Proteins by Means of the Biuret Method, J. Biol. Chem., 1949, vol. 177, pp. 751–766.PubMedGoogle Scholar
  5. 5.
    Wiechelman, K.J., Braun, R.D., and Fitzpatrick, J.D., Investigation of the Bicinchoninic Acid Protein Assay: Identification of the Groups Responsible for Color Formation, Anal. Biochem., 1988, vol. 175, pp. 231–237.PubMedCrossRefGoogle Scholar
  6. 6.
    Layne, E., Spectrophotometric and Turbidimetric Methods for Measuring Proteins, Methods Enzymol., 1957, vol. 3, pp. 447–455.CrossRefGoogle Scholar
  7. 7.
    Fasman, G.D., Practical Handbook of Biochemistry and Molecular Biology, Boca Raton: CRC, 1989.Google Scholar
  8. 8.
    Stoscheck, C.M., Quantitation of Protein, Methods Enzymol., 1990, vol. 182, pp. 50–68.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • N. S. Stepanchenko
    • 1
  • G. V. Novikova
    • 1
    Email author
  • I. E. Moshkov
    • 1
  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia

Personalised recommendations