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Role of lipoxygenase and allene oxide synthase in wound-inducible defense response of pea

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Abstract

Lipoxygenase (LOX) and allene oxide synthase (AOS) are key enzymes in the jasmonic acid (JA) biosynthesis pathway. In this work, the role of LOX and AOS in defense response induced by wounding was investigated using pea (Pisum sativum L., cv. Ning Xia) seedlings as the material. The results showed that wound-induced “JA burst” was accompanied by the activation of LOX and AOS and the accumulation of their mRNAs; applied JA also stimulated the accumulation of LOX and AOS. Further experiments conducted with inhibitors demonstrated that the wound-induced JA was regulated by LOX and AOS at both transcriptional and enzymic levels. Their activation was necessary in wound-mediated defense response and could enhance the tolerance of pea seedlings to wounding.

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Abbreviations

AOC:

allene oxide cyclase

AOS:

allene oxide synthase

ASA:

acetylsalicylic acid

JA:

jasmonic acid

HPOT:

hydroperoxyoctadecatrienoic acid

JAs:

jasmonates

LOX:

lipoxygenase

LA:

linoleic acid

LeA:

linolenic acid

MeJA:

methyl jasmonic acid

NDGA:

nordihydroguaiaretic acid

OPDA:

12-oxo-oxophytodienoic acid

OPR3:

12-oxophytodienoic acid reductase

PAL:

phenylalanine ammonialyase.

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Authors and Affiliations

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China

    H. R. Yang, K. Tang & W. D. Huang

  2. School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    H. R. Yang & K. Tang

  3. Institute of Geographic Sciences and Natural Resources Research, the Chinese Academy of Sciences, Beijing, 100101, China

    H. T. Liu

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  1. H. R. Yang
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  2. H. T. Liu
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  3. K. Tang
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  4. W. D. Huang
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Correspondence to W. D. Huang.

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This text was submitted by the authors in English.

These authors contributed equally to this work.

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Yang, H.R., Liu, H.T., Tang, K. et al. Role of lipoxygenase and allene oxide synthase in wound-inducible defense response of pea. Russ J Plant Physiol 58, 238–247 (2011). https://doi.org/10.1134/S1021443711020233

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