Abstract
Atropa belladonna L. plants were grown in water culture for 8 weeks before the nutrient medium was supplemented with NiCl2 to final concentrations of 0 (control treatment), 50, 100, 150, 200, 250, and 300 μM. After 4 days of plant growing in the presence of nickel chloride, the content of water, proline, Ni, Fe, free polyamines, as well as lipid peroxidation rates were measured. The addition of 100–150 μM Ni to the medium significantly reduced the fresh weight increments and water content in comparison with these parameters for untreated plants; 200 μM Ni caused serious, although nonlethal damage to the plants, whereas 250 and 300 μM Ni proved to be lethal. In the aboveground organs, the major part of Ni was accumulated in the apical leaves. When the plants were treated with 200 μM Ni, the Ni content in apical leaves was 220 μg/g dry wt, while Ni content in roots reached 1500 μg/g dry wt. The treatment of plants with proline in the presence of 200 μM Ni inhibited Ni accumulation in tissues. The proline-treated plants exhibited elevated iron content in leaves and especially in roots and were characterized by comparatively low rates of lipid peroxidation and by sustained leaf water status. When 200 μM Ni was applied, the content of free putrescine decreased, while the contents of spermine and spermidine in leaves increased appreciably with respect to the control values. The toxic effect of nickel was accompanied not only by an enhanced accumulation of high- molecular-weight polyamines but also by their oxidative degradation, which was evident from the 14-fold increase in the content of 1,3-diaminopropane. The protective effect of exogenous proline in the presence of high nickel concentrations was manifested in lowered lipid peroxidation rates, alleviation of iron deficiency, and in retarded oxidative degradation of polyamines.
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Abbreviations
- DAP:
-
diaminopropane
- HM:
-
heavy metal
- Spd:
-
spermidine
- Spm:
-
spermine
References
Mikroelementy v okruzhayushchei srede. Biogeokhimiya, biotekhnologiya i bioremediatsiya (Micronutrients in Environment. Bio- and Geochemistry, Biotechnology, and Bioremediation) Prasad, M.N.V., et al., Eds., Moscow: Fizmatlit, 2009.
Temp, G.A., Nickel in Plants and Its Toxicity, Ustoichivost’ k tyazhelym metallam dikorastushchikh vidov (Resistance of Wild Species to Heavy Metals), Alekseeva-Popova, N.V., Ed., Leningrad: Lenuprizdat, 1991.
Seregin, I.V. and Kozhevnikova, A.D., Physiological Role of Nickel and Its Toxic Effects on Higher Plants, Russ. J. Plant Physiol., 2006, vol. 53, pp. 257–277.
Lee, J., Reeves, R.D., Drooks, R.R., and Jaffre, T., The Relation between Nickel and Citric Acid in Some Nickel-Accumulating Plants, Phytochemistry, 1978, vol. 17, pp. 1033–1035.
Kholodova, V., Volkov, K., and Kuznetsov, Vl.V., Plants under Heavy Metal Stress in Saline Environments, Soil Heavy Metals/Soil Biol., 2010, vol. 19, pp. 163–183.
Shevyakova, N.I., Il’ina, E.N., and Kuznetsov, Vl.V., Polyamines Increase the Plant Potential of Phytoremediation during Soil Cleaning after Heavy Metal Pollution, Dokl. Akad. Nauk, 2008, vol. 423, pp. 1–4.
Shevyakova, N.I., Il’ina, E.N., Stetsenko, L.A., and Kuznetsov, Vl.V., Putrescine Increases Nickel Accumulation in Rape Shoots (Brassica napus L.) Increased by Putrescine, Int. J. Phytoremed., 2010, doi: 10.1080/152265514.2010.495147.
Farago, M.E. and Mullen, W.A., Plants which Accumulate Metals. Part IV. A Possible Cooper-Proline Complex from the Roots of Armeria maritima, Inorg. Chim. Acta, 1979, vol. 32, pp. 93–94.
Kuznetsov, Vl.V. and Shevyakova, N.I., Polyamines and Stress Tolerance of Plants (Invited Review), Plant Stress, 2007, vol. 1, pp. 50–71.
Grinkevich, N.I., Borovkova, L.I., and Gribovskaya, I.F., Effects of Microelements on Alkaloid Content in Atropa belladonna Plant, Farmatsiya, 1970, no. 5, pp. 41–47.
Ali, R.M., Role of Putrescine in Salt Tolerance of Atropa belladonna Plant, Plant Sci., 2000, vol. 152, pp. 173–179.
Khazieva, F.M. and Konon, N.T., Introduction of Atropa belladonna (Solanaceae) in Moscow Oblast, Zh. Rastit. Resursy, 2009, no. 2, pp. 31–37.
Winter, K. and Holtum, J.A.M., Environment or Development? Lifetime Net CO2 Exchange and Control of the Expression of Crassulacean Acid Metabolism in Mesembryanthemum crystallinum, Plant Physiol., 2007, vol. 143, pp. 98–107.
Golubkina, N.A., Fluorimetry for Selenium Determination, Zh. Anal. Khim., 1995, vol. 50, pp. 492–497.
Heath, R.L. and Packer, L., Photoperoxidation in Isolated Chloroplasts. Kinetics and Stoichiometry of Fatty Acid Peroxidation, Arch. Biochem. Biophys., 1968, vol. 125, pp. 189–198.
Rakitin, V.Yu., Prudnikova, O.N., Karyagin, V.V., Rakitina, T.Ya., Vlasov, P.V., Borisova, T.A., Novikova, G.V., and Moshkov, I.E., Ethylene Evolution and ABA and Polyamine Contents in Arabidopsis thaliana during UV-B Stress, Russ. J. Plant Physiol., 2008, vol. 55, pp. 321–327.
Bates, L.S., Waldren, R.P., and Teare, I.D., Rapid Determination of Free Proline for Water-Stress Studies, Plant Soil, 1973, vol. 39, pp. 205–207.
Zaitsev, G.N., Matematicheskaya statistika v eksperimental’noi botanike (Mathematic Statistics in Experimental Botany), Moscow: Nauka, 1984.
Mizuno, T., Usui, K., Horie, K., Nosaka, S., Mizuno, N., and Obata, H., Cloning of Three ZIP/Nramp Transporter Genes from a Ni Hyperaccumulator Plant Thlaspi japonicum and Their Ni2+-Transport Ability, Plant Physiol. Biochem., 2005, vol. 43, pp. 793–801.
Migocka, M. and Klobus, G., The Properties of the Mn, Ni and Pb Transport Operating at Plasma Membranes of Cucumber Roots, Physiol. Plant., 2007, vol. 129, pp. 578–587.
Grinkevich, N.I. and Sorokina, A.A., Role of Geochemical Environmental Factors in Plant Production of Biological Active Compounds, Biologicheskaya rol’ mikroelementov (Biological Role of Micronutrients), Moscow: Nauka, 1983.
Boyd, R.S. and Martens, S.N., Nickel Hyperaccumulation by Thlaspi montanum var. montanum (Brassicaceae): A Constitutive Trait, Am. J. Bot., 1998, vol. 85, pp. 259–265.
Kuznetsov, Vl.V. and Shevyakova, N.I., Proline under Stress: Biological Role, Metabolism, and Regulation, Russ. J. Plant Physiol., 1999, vol. 46, pp. 274–289.
Radyukina, N.L., Shashukova, A.V., Shevyakova, N.I., and Kuznetsov, Vl.V., Proline Involvement in the Common Sage Antioxidant System in the Presence of NaCl and Paraquat, Russ. J. Plant Physiol., 2008, vol. 55, pp. 649–656.
Shevyakova, N.I., Bakulina, E.A., and Kuznetsov, Vl.V., Proline Antioxidant Role in the Common Ice Plant Subjected to Salinity and Paraquat Treatment Inducing Oxidative Stress, Russ. J. Plant Physiol., 2009, vol. 56, pp. 663–669.
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Original Russian Text © L.A. Stetsenko, N.I. Shevyakova, V.Yu. Rakitin, Vl.V. Kuznetsov, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 2, pp. 275–282.
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Stetsenko, L.A., Shevyakova, N.I., Rakitin, V.Y. et al. Proline protects Atropa belladonna plants against nickel salt toxicity. Russ J Plant Physiol 58, 337–343 (2011). https://doi.org/10.1134/S102144371102021X
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DOI: https://doi.org/10.1134/S102144371102021X