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The isolation and analysis of a soybean CO Homologue GmCOL10

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Abstract

The CONSTANS (CO) gene is a key regulator of the response to photoperiod in the model plant Arabidopsis thaliana, and its homologues are present in many plant species. We describe here the isolation of the CO homologue for zinc finger protein gene GmCOL10 (Glycine max CONSTANS-Like 10) from the soybean cultivar Kennong18. Sequence comparisons showed that the closest A. thaliana gene to GmCOL10 is COL5. The expression of GmCOL10 was regulated in a circadian manner, especially under short-day conditions. The expression of GmCOL10 was concentrated in vegetative organs, and in particular in the unifoliolates and cotyledons. An analysis of subcellular localization found GmCOL10 in the nucleus. Our data suggested that GmCOL10 was not related to the photoperiodic pathway of floral transition as Arabidopsis CO does.

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Abbreviations

ACT11:

actin 11

CCT:

CO, CO-like, TOC1

CO:

CONSTANS

COL:

CO-like

FT:

FLOWERING LOCUS T

LD:

long day

SD:

short day

SKIP16:

SKP1/Ask-Interacting Protein 16

TUA5:

alpha-tubulin

UKN1:

hypothetical protein

ZT:

zeitgeber time

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Authors and Affiliations

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100094, China

    L. Liu & Y. Han

  2. College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China

    J. Ma & X. Chen

  3. Institute of Crop Sciences, National Key Facility of Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Y. -F. Fu

Authors
  1. L. Liu
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  2. J. Ma
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  3. Y. Han
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  4. X. Chen
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  5. Y. -F. Fu
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Correspondence to Y. -F. Fu.

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This test was submitted by the authors in English.

These authors contributed equally to the work.

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Liu, L., Ma, J., Han, Y. et al. The isolation and analysis of a soybean CO Homologue GmCOL10 . Russ J Plant Physiol 58, 330–336 (2011). https://doi.org/10.1134/S1021443711020105

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  • DOI: https://doi.org/10.1134/S1021443711020105

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