Abstract
The effects of ammonium on activity of sucrose synthase (SS) in the roots of pea (Pisum sativum L.) plants were studied. On the medium containing 14.2 mM (NH4)2SO4, SS activity increased by 20–200% for 10–20 days of plant growth as compared with the roots of plants growing without nitrogen. Illuminance affected the degree of effects. Under natural illumination, ammonium affected SS activity not only in sunny days (up to 25 klx) but also in cloudy days (3–6 klx) but to a lower degree. Under stable low light (2.5 klx), ammonium did not affect SS activity. In the in vitro experiments, at (NH4)2SO4 concentrations from 0 to 1 mM, SS activity was suppressed (up to 10%), whereas 1–37.5 mM (NH4)2SO4, it was increased (up to 50%).
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- SS:
-
sucrose synthase
References
Bruskova, R.K., Nikitin, A.V., Satskaya, M.V., and Izmailov, S.F., Effect of Nitrate on Pea Sucrose Synthase, Russ. J. Plant Physiol., 2009, vol. 56, pp. 74–79.
Champigni, M.L., Brauer, M., Bismuth, E., Cao, ThiManh, Siegl, L., Le van Quy, and Stitt, M., The Short-Term Effect of and Assimilation on Sucrose Synthesis in Leaves, J. Plant Physiol., 1992, vol. 139, pp. 361–368.
Yamamoto, J. and Ishimoto, M., Effect of Nitrate Reduction on the Enzyme Levels in Carbon Metabolism in Escherichia coli, J. Biochem., 1975, vol. 78, pp. 307–315.
Bruskova, R.K., Zartdinova, R.F., Satskaya, M.V., and Izmailov, S.F., Activities of Sucrose Synthase and Acid Invertase in Pea Seedling Organs, Russ. J. Plant Physiol., 2004, vol. 51, pp. 631–635.
Pavlinova, O.A. and Prasolova, M.F., Sucrose Synthesizing Enzymes in Sugar Beet Root, Sov. Plant Physiol., 1970, vol. 17, pp. 295–301.
Perry, C., Leigh, R.A., Tomas, A.R., Wyse, R.E., and Hall, J.L., The Regulation of Turgor Pressure during Sucrose Mobilization and Salt Accumulation by Excised Root Tissue of Red Beet, Planta, 1987, vol. 170, pp. 353–361.
Platt, S.G., Plaut, Z., and Bassham, I.A., Ammonia Regulation of Carbon Metabolism in Photosynthesizing Leaf Discs, Plant Physiol., 1977, vol. 60, pp. 739–772.
Paul, J.S., Cornwell, K.L., and Bassham, J.A., Effect of Ammonia on Carbon Metabolism in Photosynthesizing Isolated Mesophyll Cells from Papaver somniferum L., Planta, 1978, vol. 142, pp. 49–54.
Andreeva, T.F., Photosynthesis and Nitrogen Exchange in Plants, Fiziologiya fotosinteza (Physiology of Photosynthesis), Nichiporovich, A.A., Ed., Moscow: Nauka, 1982.
Velikodnaia, V.G., Sarishvili, N.G., and Datunashvili, E.N., Effect of Ammonium on Certain Oxidoreductases of Wine Yeasts, Mikrobiologia, 1975, vol. 44, pp. 800–803.
Nomura, M., Mai, H.T., Fujii, M., Hata, S., Izui, K., and Tajima, S., Phosphoenolpyruvate Carboxylase Plays a Crucial Role in Limiting Nitrogen Fixation in Lotus japonicus Nodules, Plant Cell Physiol., 2006, vol. 47, pp. 613–621.
Galvez, S., Lancien, M., and Hodges, M., Are Isocitrate Dehydrogenases and 2-Oxoglutarate Involved in the Regulation of Glutamate Synthesis? Trends Plant Sci., 1999, vol. 4, pp. 484–490.
Hodges, M., Enzyme Redundancy and the Importance of 2-Oxoglutarate in Plant Ammonium Assimilation, J. Exp. Bot., 2002, vol. 53, pp. 905–916.
Curatti, L., Giarrocco, L., and Salerno, G.L., Sucrose Synthase and RuBisCo Expression Is Similarly Regulated by the Nitrogen Source in the Nitrogen-Fixing Cyanobacterium Anabaena sp, Planta, 2006, vol. 223, pp. 891–900.
Komina, O., Zhou, Y., Sarath, G., and Chollet, R., In Vivo and in Vitro Phosphorylation of Membrane and Soluble Forms of Soybean Nodule Sucrose Synthase, Plant Physiol., 2002, vol. 129, pp. 1664–1673.
Qiu, Q.S., Hardin, S.C., Mace, J., Brutnell, T.P., and Huber, S.C., Light and Metabolic Signals Control the Selective Degradation of Sucrose Synthase in Maize Leaves during Deetiolation, Plant Physiol., 2007, vol. 144, pp. 468–478.
Sasaki, Y., Sakihama, T., Kamikubo, T., and Shinozaki, K., Phytochrome-Mediated Regulation of Two mRNAs, Encoded by Nuclei and Chloroplasts of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase, Eur. J. Biochem., 1983, vol. 133, pp. 617–620.
Yamamoto, N., Mukai, Y., Matsuoka, M., KanoMurakami, Y., Tanaka, Y., Ohashi, Y., Ozeki, Y., and Odani, K., Ligh-Independent Expression of Cab and rbcS Genes in Dark-Grown Pine Seedlings, Plant Physiol., 1991, vol. 95, pp. 379–383.
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Original Russian Text © A.V. Nikitin, R.K. Bruskova, T.M. Andreeva, S.F. Izmailov, 2010, published in Fiziologiya Rastenii, 2010, Vol. 57, No. 1, pp. 76–80.
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Nikitin, A.V., Bruskova, R.K., Andreeva, T.M. et al. Effect of ammonia on sucrose synthase in pea roots. Russ J Plant Physiol 57, 69–73 (2010). https://doi.org/10.1134/S1021443710010097
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DOI: https://doi.org/10.1134/S1021443710010097


