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Increased activity of ATP-sulfurylase and increased contents of cysteine and glutathione reduce high cadmium-induced oxidative stress in mustard cultivar with high photosynthetic potential

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Abstract

Cadmium is known to reduce photosynthesis and overall growth of plants. Plants adopt several mechanisms of Cd detoxification, such as accumulation of sulfur-rich compounds, like glutathione (GSH) and its precursor cysteine. The accumulation of GSH is regulated by the activity of ATP-sulfurylase, a rate-limiting enzyme in sulfur assimilation. The carbon of Cys is provided through photosynthesis. Thus, a plant with the higher photosynthetic potential and ATP-sulfurylase activity may have the higher contents of Cys and GSH and therefore may provide for a greater tolerance to Cd stress. Mustard (Brassica juncea L. Czern and Coss.) cvs. Varuna (high photosynthetic potential) and RH30 (low photosynthetic potential) were subjected to 0 and 200 mg Cd/kg soil, and the activity of ATP-sulfurylase, the contents of Cys and GSH, oxidative stress, and activities of antioxidant enzymes were studied. Under 200 mg Cd/kg soil, cv. Varuna showed an increased ATP-sulfurylase activity, the higher contents of Cys and GSH, and the net photosynthetic rate than cv. RH30. In contrast, the activity of superoxide dismutase, the contents of thiobarbituric acid-reactive substances, and H2O2, and electrolyte leakage were found to be greater in cv. RH30 showing an increased oxidative stress than cv. Varuna. However, the activities of ascorbate peroxidase and glutathione reductase were greater in cv. Varuna than cv. RH30. The results show that a greater ATP-sulfurylase activity, an enhanced production of Cys and GSH, and an efficient antioxidant enzyme system in the high photosynthetic mustard cv. Varuna helped to the reduce the oxidative stress maintaing high photosynthesis.

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Abbreviations

APX:

ascorbate peroxidase

Cd:

cadmium

DAS:

days after sowing

GR:

glutathione reductase

g s :

stomatal conductance

GSH:

glutathione reduced

C i :

intercellular CO2 concentration

P N :

net photosynthetic rate

PCs:

phytochelatins

SOD:

superoxide dismutase

TBARS:

thiobarbituric acid-reactive substances

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Authors and Affiliations

  1. Department of Botany, Aligarh Muslim University, Aligarh, 202002, India

    N. A. Khan, N. A. Anjum, R. Nazar & N. Iqbal

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  1. N. A. Khan
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  2. N. A. Anjum
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Correspondence to N. A. Khan.

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Published in Russian in Fiziologiya Rastenii, 2009, Vol. 56, No. 5, pp. 743–750.

This text was submitted by the authors in English.

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Khan, N.A., Anjum, N.A., Nazar, R. et al. Increased activity of ATP-sulfurylase and increased contents of cysteine and glutathione reduce high cadmium-induced oxidative stress in mustard cultivar with high photosynthetic potential. Russ J Plant Physiol 56, 670–677 (2009). https://doi.org/10.1134/S1021443709050136

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  • DOI: https://doi.org/10.1134/S1021443709050136

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