Recovery of bean plants from boron-induced oxidative damage by zinc supply
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The effects of zinc on growth, boron uptake, lipid peroxidation, membrane permeability (MP), lypoxygenase (LOX) activity, proline and H2O2 accumulation, and the activities of major antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX)) in bean plants were investigated under greenhouse conditions. Treatments consisted of control, 20 mg/kg B, and 20 mg/kg B plus 20 mg/kg Zn. When the plants were grown with 20 mg/kg Zn, B toxicity was less severe. Zinc supplied to soil counteracted the deleterious effects of B on root and shoot growth. Excess B significantly increased and Zn treatment reduced B concentrations in shoot and root tissues. Applied Zn increased the Zn concentration in the roots and shoots. While the concentrations of H2O2 and proline were increased by B toxicity, their concentrations were decreased by Zn supply. Boron toxicity increased the MP, malondialdehyde content, and LOX activity in excised bean leaves. Applied Zn significantly ameliorated the membrane deterioration. Compared with control plants, the activity of SOD was increased while that of CAT was decreased and APX remained unchanged in B-stressed plants. However, application of Zn decreased the SOD and increased the CAT and APX activities under toxic B conditions. It is concluded that Zn supply alleviates B toxicity by preventing oxidative membrane damage.
Key wordsPhaseolus vulgaris antioxidant enzymes B toxicity oxidative stress proline zinc
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- 1.Torun, A., Gultekin, I., Kalayci, M., Yilmaz, A., Eker, S., and Cakmak, I., Effects of Zinc Fertilization on Grain Yield and Shoot Concentrations of Zinc, Boron, and Phosphorus of 25 Wheat Cultivars Grown on a Zinc Deficient and Boron-Toxic Soil, J. Plant Nutr., 2001, vol. 24, pp. 1817–1829.CrossRefGoogle Scholar
- 2.Cakmak, I. and Marschner, H., Increase in Membrane Permeability and Exudation in Roots of Zinc Deficient Plants, J. Plant Physiol., 1988, vol. 132, pp. 356–361.Google Scholar
- 11.Singh, J.P., Dahiya, D.J., and Narwai, R.P., Boron Uptake and Toxicity in Relation to Zinc Supply, Nutr. Cyc. Agroec., 1990, vol. 24, pp. 105–110.Google Scholar
- 13.Marschner, H. and Cakmak, I., High Light Intensity Enhances Chlorosis and Necrosis in Leaves of Zn, K and Mg Deficient Plants, J. Plant Physiol., 1989, vol. 134, pp. 308–315.Google Scholar
- 18.Nakano, Y. and Asada, K., Hydrogen Peroxide Is Scavenged by Ascorbate-Specific Peroxidase in Spinach Chloroplasts, Plant Cell Physiol., 1981, vol. 22, pp. 867–880.Google Scholar
- 20.Axelrod, B., Cheesbrough, T.M., and Laakso, S., Lipoxigenases from Soybeans, Methods Enzymol., Lowenstein, J.M., Ed., New York: Academic, 1981, pp. 441–451.Google Scholar
- 36.Garcia, P.O.C., Rivero, R.M., Lopez-Lefebre, L.R., Sanchez, E., Ruiz, J.M., and Romero, L., Response of Oxidative Metabolism to the Application of Carbendazim plus Boron in Tobacco, Aust. J. Plant Physiol., 2001, vol. 28, pp. 801–806.Google Scholar