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Russian Journal of Plant Physiology

, Volume 56, Issue 3, pp 377–388 | Cite as

Activity of biochemical pH-stat enzymes in cereal root tips under oxygen deficiency

  • K. Yu. KulichikhinEmail author
  • T. V. Chirkova
  • K. V. Fagerstedt
Research Papers

Abstract

The activity of the enzymes of alcoholic and lactic-acid fermentation: pyruvate decarboxylase (PDC, EC 4.1.1.1), alcohol dehydrogenase (ADH, EC 1.1.1.1), lactate dehydrogenase (LDH, EC 1.1.1.27) and the enzymes of malic acid metabolism: phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.23), NAD-dependent malate dehydrogenase (NAD-MDH, EC 1.1.1.37), and NADP-dependent malic enzyme (NADP-ME, EC 1.1.1.40) involved in the operation of biochemical pH-stat was investigated in the root tips of wheat (Triticum aestivum L.) and rice (Oryza sativa L.) under hypoxia and anoxia. Exposures lasted for 6, 12, and 18 h. The most pronounced response was detected for the enzymes of alcoholic fermentation. The activation of ADH and PDC in wheat occurred only under hypoxia, whereas in rice it was detected both under hypoxia and anoxia. The activation of LDH in wheat occurred under hypoxia, and in rice, the activity of this enzyme was slightly enhanced. The activity of the enzymes of malic acid metabolism did not change except in wheat root tips under hypoxia when PEPC activity decreased and NADP-ME activity simultaneously rose. The role of biochemical pH-stat in the regulation of cytoplasmic pH in plant cells under oxygen deficit and the mechanisms for regulating the activities of enzymes involved in biochemical pH-stat are discussed as well as the interaction between biochemical pH-stat and other mechanisms maintaining pH of plant cells. The results are analyzed within a context of intracellular pH regulation.

Key words

Oryza sativa Triticum aestivum root tip hypoxia anoxia biochemical pH-stat 

Abbreviations

ADH

alcohol dehydrogenase

LDH

lactate dehydrogenase

MDH-NAD

dependent malate dehydrogenase

NADP-ME-NADP

dependent decarboxylating malate dehydrogenase (malic enzyme)

NTP

nucleotide triphosphates

PDC

pyruvate decarboxylase

PEPC

phosphoenolpyruvate carboxylase

pHcyt

cytoplasmic pH

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • K. Yu. Kulichikhin
    • 1
    • 2
    Email author
  • T. V. Chirkova
    • 3
  • K. V. Fagerstedt
    • 1
  1. 1.Department of Biological and Ecological SciencesUniversity of HelsinkiHelsinkiFinland
  2. 2.Laboratory of Photosynthesis, Biological Research InstituteSt. Petersburg State UniversitySt. PetersburgRussia
  3. 3.Department of Plant Physiology and BiochemistrySt. Petersburg State UniversitySt. PetersburgRussia

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