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Changes in the composition of ionogenic groups of the wall of the lily pollen grain during germination

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Abstract

Changes in the composition of ionogenic groups of the polymeric matrix of the cell walls of lily (Lilium longiflorum Thunb.) pollen grains were studied during its activation at the early stages of pollen germination. In the cell walls isolated from nonactivated and activated pollen grains, four types of ionogenic groups were identified: amino groups, carboxylic groups of uronic acids, phenolic OH-groups. and groups with pKa 7–8. During the early stages of germination, ionization constants of each type groups remained unchanged, but the quantitative composition of ionogenic groups in the intine changed. In this matrix, a decrease in the content of phenolic groups and demethylated carboxylic groups of uronic acids was detected. It is supposed that, at early stages of germination, the intine loses some part of acid pectins and some phenolic compounds.

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Abbreviations

AO:

acridine orange

PME:

pectin methyl esterase

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Authors and Affiliations

  1. Department of Plant Physiology, Faculty of Biology, Moscow State University, Moscow, Russia

    N. R. Meychik, A. V. Smirnova, N. P. Matveeva, Yu. I. Nikolaeva & I. P. Ermakov

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  1. N. R. Meychik
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  2. A. V. Smirnova
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  3. N. P. Matveeva
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  4. Yu. I. Nikolaeva
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  5. I. P. Ermakov
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Correspondence to N. R. Meychik.

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Original Russian Text © N.R. Meychik, A.V. Smirnova, N.P. Matveeva, Yu.I. Nikolaeva, I.P. Ermakov, 2009, published in Fiziologiya Rastenii, 2009, Vol. 56, No. 2, pp. 232–240.

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Meychik, N.R., Smirnova, A.V., Matveeva, N.P. et al. Changes in the composition of ionogenic groups of the wall of the lily pollen grain during germination. Russ J Plant Physiol 56, 211–218 (2009). https://doi.org/10.1134/S1021443709020095

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  • DOI: https://doi.org/10.1134/S1021443709020095

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