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Interaction of gibberellic acid and 24-epibrassinolide in the regulation of Arabidopsis thaliana seedling scotomorphogenesis

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Abstract

The effects of GA3, 24-epibrassinolide (EBL), and their combination on morphogenesis of Arabidopsis thaliana (L.) Heynh seven-day-old seedlings were studied. Four plant lines were analyzed: wild type Ler and ga4-1 mutant, belonging to the Landsberg erecta ecotype and wild type Col and det2 mutant, both of the Columbia ecotype. In ga4-1 and det2, GA4/1-and brassinosteroid-deficient mutants, the highest hypocotyl growth response to the lack of hormones was noted. The cotyledon shape and size were dependent on EBL, and the root length was both GA3-and EBL-regulated, indicating organ specificities in the responses to these hormones. Simultaneous treatment of dark-grown plants with GA3 and EBL exerted an additive stimulatory effect on the root growth of det2, reduced the inhibitory effect of EBL on hypocotyl elongation of ga4-1, and enhanced the effect of EBL on hypocotyl and cotyledon elongation of det2.

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Abbreviations

BR(s):

brassinosteroid(s)

EBL:

24-epibrassinolide

MS:

Murashige and Skoog nutrient medium

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Authors and Affiliations

  1. Department of Plant Physiology and Biotechnology, Tomsk State University, pr. Lenina 36, Tomsk, 634050, Russia

    I. F. Golovatskaya

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  1. I. F. Golovatskaya
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Correspondence to I. F. Golovatskaya.

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Original Russian Text © I.F. Golovatskaya, 2008, published in Fiziologiya Rastenii, 2008, Vol. 55, No. 5, pp. 738–745.

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Golovatskaya, I.F. Interaction of gibberellic acid and 24-epibrassinolide in the regulation of Arabidopsis thaliana seedling scotomorphogenesis. Russ J Plant Physiol 55, 663–669 (2008). https://doi.org/10.1134/S1021443708050105

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