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Light absorption and scattering by cell suspensions of some cyanobacteria and microalgae

  • M. N. MerzlyakEmail author
  • O. B. Chivkunova
  • I. P. Maslova
  • K. R. Naqvi
  • A. E. Solovchenko
  • G. L. Klyachko-Gurvich
Methods

Abstract

Absorption spectra of cyanobacteria (Anacystis nidulans, Anabaena variabilis, and Chlorogloeopsis fritschii), red (Cyanidium caldarum and Porphyridium cruentum), green (Dunaliella maritima and Dunaliella salina) and diatom (Thalassiosira weisflogii) alga cell suspensions are presented; the spectra were obtained by using an approach developed earlier to compensate for scattering [1, 2]. In all species, the shapes of the absorption spectra were independent of the cell concentration. For Th. weisflogii and D. maritima, the analysis of selective and nonselective scattering was carried out. The effect of mechanical cell disruption on optical properties (absorption, scattering, and “package” effect) on D. maritima was studied. The character and dynamics of optical changes in D. salina under the influence of sodium chloride were followed.

Key words

cyanobacteria diatom algae green algae red algae absorption scattering spectrophotometry 

Abbreviations

Chl

chlorophyll

D

attenuance (optical density)

IS

integrating sphere

NIR

Near Infra Red

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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • M. N. Merzlyak
    • 1
    Email author
  • O. B. Chivkunova
    • 1
  • I. P. Maslova
    • 2
  • K. R. Naqvi
    • 3
  • A. E. Solovchenko
    • 1
  • G. L. Klyachko-Gurvich
    • 2
  1. 1.Faculty of BiologyMoscow State UniversityMoscowRussia
  2. 2.Timiriazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  3. 3.Department of PhysicsNorwegian University of Science and Technology (NTNU)TrondheimNorway

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