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Improving Glycyrrhiza uralensis salt tolerance with N+ ion irradiation

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Abstract

Low energy (25 keV) N+ ions were implanted into liquorice (Glycyrrhiza uralensis) seeds at a fluency of either zero (control) or 900 × (2.6 × 1013) ions/cm2. After irradiation, all the seeds were planted in the plastic pots for a growth period of one month. Thereafter, the seedlings in the pot were subjected to saline stress at 600 mM for about 3 days. The morphological and physiological characteristics such as total chlorophyll content, proline level, activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ATPase, and triphenyl tetrazolium chloride (TTC) reduction in seedling roots were investigated. Our results indicated that ion irradiation significantly increased the shoot height, leaflet number, taproot lenght, lateral root number, and shoot and root weights of liquorice seedlings with or without saline stress. Furthermore, the total chlorophyll content, proline level, SOD, POD, CAT, ATPase activities, and root TTC reduction vigor of seedlings were all found to be significantly increased under saline stress by ion irradiation compared with their corresponding controls. These results indicated that ion irradiation can strengthen the resistance of liquorice seedlings to saline stress and may have a potential application for the improvement of plants in sand areas.

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Abbreviations

CAT:

catalase

POD:

peroxidase

SOD:

superoxide dismutase

TTC:

2,3,5-triphenyltetrazolium chloride

References

  1. Ramoliya, P.J. and Pandey, A.N., Effect of Increasing Salt Concentration on Emergence, Growth and Survival of Seedlings of Salvadora oleoides (Salvadoraceae), J. Arid Environ., 2002, vol. 51, pp. 121–132.

    Article  Google Scholar 

  2. Schabes, F.I. and Sigstad, E.E., Calorimetric Studies of Quinoa (Chenopodium quinoa Willd.) Seed Germination under Saline Stress Conditions, Thermochim. Acta, 2005, vol. 428, pp. 71–75.

    Article  CAS  Google Scholar 

  3. Wang, Y.Q. and He, R.X., Analysis on Glycyrrhiza uralensis Fisch and Soil Desertification, J. Eco-Agron., 2004, vol. 12, pp. 194–195.

    Google Scholar 

  4. Asish, K.P. and Anath, B.D., Salt Tolerance and Salinity Effects on Plants: A Review, Ecotoxic. Environ. Safety, 2005, vol. 60, pp. 324–349.

    Article  Google Scholar 

  5. Meloni, D.A., Oliva, M.A., and Carlos, A., Photosynthesis and Activity of Superoxide Dismutase, Peroxidase and Glutathione Reductase in Cotton under Salt Stress, Environ. Exp. Bot., 2003, vol. 49, pp. 69–76.

    Article  CAS  Google Scholar 

  6. Sunyo, J., Variation in Antioxidant Metabolism of Young and Mature Leaves of Arabidopsis thaliana Subjected to Drought, Plant Sci., 2004, vol. 166, pp. 459–466.

    Article  Google Scholar 

  7. Rene, M., Erika, N., Barbel, L., Steffi, H., Susan, F., Waltraud, S., Aurora, N., and Hans, B., Improvement of Tolerance to Paraquat and Drought in Barley (Hordeum vulgare L.) by Exogenous 2-Aminoethanol: Effects on Superoxide Dismutase Activity and Chloroplast Ultrastructure, Plant Sci., 2005, vol. 168, pp. 691–698.

    Article  Google Scholar 

  8. David, R.D. and Jack, M.W., Osmotic Induced Stimulation of the Reduction of the Viability Dye 2,3,5-Triphenyltetrazolium Chloride by Maize Roots and Callus Cultures, J. Plant Physiol., 2004, vol. 161, pp. 397–403.

    Article  Google Scholar 

  9. Arora, A., Sairam, R.K., and Srivastava, G.C., Oxidative Stress and Antioxidative Systems in Plants, Curr. Sci., 1982, vol. 57, pp. 1227–1238.

    Google Scholar 

  10. Mokhamed, A.M., Raldugina, G.N., Kholodova, V.P., and Kuznetsov., Vl.V., Osmolyte Accumulation in Different Rape Genotypes under Sodium Chloride Salinity, Russ. J. Plant Physiol., 2006, vol. 53, pp. 649–655.

    Article  CAS  Google Scholar 

  11. Popova, L.G., Kornilova, A.G., Shumkova, G.A., Andreev, I.M., and Balnokin, Yu.V., Na+-Transporting ATPase in the Plasma Membrane of Halotolerant Microalga Dunaliella maritima Operates as a Na+ Uniporter, Russ. J. Plant Physiol., 2006, vol. 53, pp. 474–480.

    Article  CAS  Google Scholar 

  12. Ashraf, M., Salt Tolerance of Cotton: Some New Advances, Crit. Rev. Plant Sci., 2004, vol. 21, pp. 1–30.

    Article  Google Scholar 

  13. Yu, Z.L., Qiu, L.J., and Huo, Y.P., Progress in Studies of Biological Effect and Crop Breeding Induced by Ion Irradiation, J. Anhui Agric. Coll., 1991, vol. 18, pp. 251–257.

    Google Scholar 

  14. Yu, Z.L., Introduction to Ion Beam Biotechnology, New York: Springer-Verlag, 2006.

    Google Scholar 

  15. Yang, M.Y., Yang, F., and Wu, Z.H., The Effect of Ar+ Ion Beam Injection on Seedling Growth and POX of Japonica Rice, J. Jilin Agric. Univ., 2001, vol. 23, pp. 37–40.

    CAS  Google Scholar 

  16. Wang, W.D., Su, M.J., and Wen, J., Effects of Ion Irradiation on Germination Percentage of Wheat Seeds and GS Vigor of the Seedlings, Henan Agric. Sci., 2004, vol. 12, pp. 18–21.

    Google Scholar 

  17. Lin, G.P., Yin, F.S., and Li, J.Z., Effect of Ion Beam Implantation on Seed Germination of Nicotia Seeds, J. Anhui Agric. Univ., 1994, vol. 22, pp. 268–270.

    Google Scholar 

  18. Huang, Z.W., Zhao, J.J., and Chang, S.H., The Effect of N+ Ion Beam Implantation on Seedling Growth and POD Iso-Enzyme of Maize, Henan Agric. Sci., 2004, vol. 12, pp. 11–14.

    Google Scholar 

  19. Liang, Q.X., Wang, F.Z., and Liu, L.A., The Preliminary Study of Low Energy Ion Beam Irradiation on Tomato Seeds, Henan Agric. Sci., 2003, vol. 7, pp. 40–42.

    Google Scholar 

  20. Wu, Y.J., Wu, J.D., Lu, G.F., Wu, J.D., Yu, Z.L., Deng, J.D., and He, J.J., Studies on the Free Radicals and SOD Enzyme of Germinating Rice Seeds under Ion Beam Irradiation, Acta Agric. Sinica, 1996, vol. 22, pp. 320–324.

    Google Scholar 

  21. Guo, J.H., Xie, C.X., Xu, J., Hu, C.Z., and Yu, Z.L., Effect of N+ Ion Implantation on Seed Vigor of Soybean and Some Antioxidase Activity in Soybean Seedling, Acta Laser Biol. Sinica, 2003, vol. 12, pp. 368–372.

    CAS  Google Scholar 

  22. Reddy, A.R., Chaitanya, K.V., and Jutur, P.P., Differential Antioxidative Responses to Water Stress among Five Mulberry (Morus alba L.) Cultivars, Environ. Exp. Bot., 2004, vol. 52, pp. 33–42.

    Article  CAS  Google Scholar 

  23. Zhao, K.F., Fan, H., and Zhou, S., Study on the Salt and Drought Tolerance of Suaeda salsa and Kalanchoe daigremontiana under Iso-Osmotic Salt and Water Stress, Plant Sci., 2003, vol. 165, pp. 837–844.

    Article  CAS  Google Scholar 

  24. Zhu, M.J., Chen, J., and Zhu, Q.H., Identification of Plasma Membrane of Maize Roots by Two-Phase Partition, Chin. Biochem., 1997, no. 6, pp. 686–690.

  25. Wei, S.L. and Zhang, X.S., Studies on Effect of N+ Ion Beam Implantation on Some Drought Tolerant Characteristics, Nucleic Tech., 2004, vol. 27, pp. 824–827.

    Google Scholar 

  26. Okusanya, O.T. and Ungar, I.A., The Growth and Mineral Composition of Three Species of Spergularia as Affected by Salinity and Nutrients at High Salinity, Am. J. Bot., 1984, vol. 71, pp. 439–447.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. College of Life Science, Yangtze University, Jingzhou, 434025, China

    X. Zhang & T. Xiong

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  1. X. Zhang
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  2. T. Xiong
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Correspondence to X. Zhang.

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Original Russian Text © X. Zhang, T. Xiong, 2008, published in Fiziologiya Rastenii, 2008, Vol. 55, No. 3, pp. 381–386.

This text was submitted by the authors in English.

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Zhang, X., Xiong, T. Improving Glycyrrhiza uralensis salt tolerance with N+ ion irradiation. Russ J Plant Physiol 55, 344–349 (2008). https://doi.org/10.1134/S1021443708030096

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  • DOI: https://doi.org/10.1134/S1021443708030096

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