Abstract
Coloration and color pattern are basic information for plant physiology, ecology, and taxonomy. Until recently, such color analysis of biological materials required expensive equipment. Here we report a convenient method for analyzing color of plant leaves with ImageJ, free software. Color digital images of croton leaves were divided with ImageJ into Red, Green, and Blue images, and then exported as two-dimensional numeric data. After processing images with Adobe Photoshop Elements and Microsoft Excel, color patterns were obtained as histograms. A comparison of the histograms evaluated characteristics of croton leaf variegation. The method successfully quantified color information of the plant leaves with an inexpensive PC set. Apart from taxonomic applications, the simple algorithms allow application of the present methodology to various types of color and color pattern analyses.
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Abbreviations
- B:
-
blue
- G:
-
green
- R:
-
red
References
Lev-Yadun, R., Inbar, M., Izhaki, I., Ne’eman, G., and Dafni, A., Colour Patterns in Vegetative Parts of Plants Deserve More Research Attention, Trends Plant Sci., 2002, vol. 7, pp. 59–60.
Lev-Yadun, R., Dafni, A., Flaishman, M.A., Inbar, M., Izhaki, I., Katzir, G., and Ne’eman, G., Plant Coloration Undermines Herbivorous Insect Camouflage, BioEssays, 2004, vol. 26, pp. 1126–1130.
Stintzing, F.C. and Carle, R., Functional Properties of Anthocyanins and Betalains in Plants, Food, and in Human Nutrition, Trends Food Sci. Technol., 2004, vol. 15, pp. 19–38.
Yamasaki, H., A Function of Colour, Trends Plant Sci., 1997, vol. 2, pp. 7–8.
Harborne, J.B. and Williams, C.A., Advances in Flavonoid Research since 1992, Phytochemistry, 2000, vol. 55, pp. 481–504.
Pietrini, F., Iannelli, M.A., and Massacci, A., Anthocyanin Accumulation in the Illuminated Surface of Maize Leaves Enhances Protection from Photoinhibitory Risks at Low Temperature, without Further Limitation to Photosynthesis, Plant Cell Environ., 2002, vol. 25, pp. 1251–1259.
Takahashi, S., Tamashiro, Y., Sakihama, Y., Yamamoto, Y., Kawamitsu, Y., and Yamasaki, H., High-Susceptibility of Photosynthesis to Photoinhibition in the Tropical Plant Ficus microcarpa L. f. cv. Golden Leaves, BMC Plant Biol., 2002, vol. 2, p. 2.
Clement, J.S. and Mabry, T.J., Pigment Evolution in the Caryophyllales: A Systematic Overview, Bot. Acta, 1996, vol. 109, pp. 360–367.
Francis, F.J., Quality as Influenced by Color, Food Quality and Preference, 1995, vol. 6, pp. 149–155.
Majidi, B. and Moshiri, B., Industrial Assessment of Horicultural Products’ Quality Using Image Data Fusion, Fusion 2003 (Proc. 6th Int. Conf. Information Fusion), pp. 868–873.
Farzad, M., Griesbach, R., Hammond, J., Weiss, M.R., and Elmendorf, H.G., Differential Expression of Three Key Anthocyanin Biosynthetic Genes in a Color-Changing Flower, Viola cornuta cv. Yesterday, Today and Tomorrow, Plant Sci., 2003, vol. 165, pp. 1333–1342.
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Published in Russian in Fiziologiya Rastenii, 2006, Vol. 53, No. 1, pp. 139–147.
The text was submitted by the authors in English.
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Shimoji, H., Tokuda, G., Tanaka, Y. et al. A simple method for two-dimensional color analyses of plant leaves. Russ J Plant Physiol 53, 126–133 (2006). https://doi.org/10.1134/S102144370601016X
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DOI: https://doi.org/10.1134/S102144370601016X