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Molecular mass characteristics and hydrodynamic and conformational properties of hyperbranched poly-L-lysines


Hydrodynamic and conformational properties of hyperbranched poly(amino acids) based on lysine have been studied by static and dynamic light scattering, velocity sedimentation, translational diffusion, and viscometry in dilute aqueous-saline solutions (0.2 M NaCl). The effects of synthesis conditions of hyperbranched poly(amino acids), modification of their end groups by histidine fragments, and incorporation of diacylated lysine residues between branching points of oligomers and polymers composed of lysine and glutamic acid on the molecular mass characteristics of the hyperbranched polymers have been ascertained. The hydrodynamic properties of the hyperbranched poly(amino acids) differ appreciably from the behavior of both linear polylysine and lysine dendrimers due to conformational features of their macromolecules.

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  1. 1.

    M. C. Morris, L. Chaloin, F. Heitz, and G. Davita, Curr. Opin. Biotechnol. 11, 461 (2000).

    Article  CAS  Google Scholar 

  2. 2.

    M. Ohsaki, T. Okuda, A. Wada, et al., Bioconjugate Chem. 13, 510 (2002).

    Article  CAS  Google Scholar 

  3. 3.

    T. Okuda, S. Kidoaki, M. Ohsaki, et al., Org. Biomol. Chem. 1, 1270 (2003).

    Article  CAS  Google Scholar 

  4. 4.

    G. P. Vlasov, V. I. Korol’kov, G. A. Pankova, et al., Bioorg. Khim. 30, 12 (2004).

    Article  CAS  Google Scholar 

  5. 5.

    T. Okuda, A. Sugiyama, T. Niidome, and H. Aoyagi, Biomaterials 25, 537 (2004).

    Article  CAS  Google Scholar 

  6. 6.

    G. P. Vlasov, V. I. Korol’kov, I. A. Gur’yanov, et al., Bioorg. Khim. 31, 167 (2005).

    CAS  Google Scholar 

  7. 7.

    G. P. Vlasov, G. M. Pavlov, N. V. Bayanova, et al., Dokl. Akad. Nauk 399, 366 (2004).

    Google Scholar 

  8. 8.

    G. P. Vlasov, I. I. Tarasenko, S. V. Valueva, et al., Polymer Science, Ser. A 47, 422 (2005) [Vysokomol. Soedin., Ser. A 47, 731 (2005)].

    Google Scholar 

  9. 9.

    G. P. Vlasov, A. P. Filippov A.P., I. I. Tarasenko, et al., Polymer Science, Ser. A 50(2008) [Vysokomol. Soedin., Ser. A 50, 589 (2008)].

  10. 10.

    W. Daly and D. Poche, Tetrahedron Lett. 29, 5859.

  11. 11.

    V. N. Tsvetkov, V. E. Eskin, and S. Ya. Frenkel’, The Structure of Macromolecules in Solutions (Nauka, Moscow, 1964) [in Russian].

    Google Scholar 

  12. 12.

    V. N. Tsvetkov, Rigid-Chain Polymers (Nauka, Leningrad, 1986; Plenum, New York, 1989).

    Google Scholar 

  13. 13.

    E. B. Tarabukina, A. A. Shpyrkov, D. V. Potapova, et al., Polymer Science, Ser. A 47, 1304 (2005) [Vysokomol. Soedin., Ser. A 47, 2157 (2005)].

    Google Scholar 

  14. 14.

    E. B. Tarabukina, A. A. Shpyrkov, D. V. Potapova, et al., Polymer Science, Ser. A 48, 974 (2006) [Vysokomol. Soedin., Ser. A 48, 1655 (2006)].

    Article  Google Scholar 

  15. 15.

    E. B. Tarabukina, A. A. Shpyrkov, E. V. Tarasova, et al., Polymer Science, Ser. A 50 (2008).

  16. 16.

    V. N. Tsvetkov and S. I. Klenin, Dokl. Akad. Nauk SSSR 88, 49 (1953).

    CAS  Google Scholar 

  17. 17.

    V. N. Tsvetkov, P. N. Lavrenko, and S. V. Bushin, Usp. Khim. 51, 1698 (1982).

    CAS  Google Scholar 

  18. 18.

    G. M. Pavlov, E. V. Korneeva, S. A. Nepogod’ev, et al., Polymer Science, Ser. A 40, 1282 (1998) [Vysokomol. Soedin., Ser. A 40, 2056 (1998)].

    Google Scholar 

  19. 19.

    V. E. Eskin, Light Scattering by Polymer Solutions (Nauka, Leningrad, 1986) [in Russian].

    Google Scholar 

  20. 20.

    E. Anufrieva, T. Ananieva, N. Bayanova, et al., Macromol. Symp. 237, 1 (2006).

    Article  CAS  Google Scholar 

  21. 21.

    E. V. Anufrieva, M. G. Krakovyak, T. D. Anan’eva, et al., Polymer Science, Ser. A 49, 671 (2006) [Vysokomol. Soedin., Ser. A 49, 1013 (2006)].

    Article  Google Scholar 

  22. 22.

    G. Vlasov, I. Tarasenko, G. Pankova, et al., in Proceedings of 5 International Dendrimer Symposium, Toulouse, 2007, p. O–74.

  23. 23.

    S. D. Zaitsev, A. A. Turshatov, G. M. Pavlov, et al., Polymer Science, Ser. B 46, 241 (2004) [Vysokomol. Soedin., Ser. B 46, 1443 (2004)].

    Google Scholar 

  24. 24.

    T. H. Mourey, S. R. Turner, M. Rubinstein, et al., Macromolecules 25, 2401 (1992).

    Article  CAS  Google Scholar 

  25. 25.

    I. B. Rietveld and J. A. M. Smith, Macromolecules 32, 4608 (1999).

    Article  CAS  Google Scholar 

  26. 26.

    R. Scherrenberg, B. Coussens, P. Vliet, et al., Macromolecules 31, 456 (1998).

    Article  CAS  Google Scholar 

  27. 27.

    G. M. Pavlov, E. V. Korneeva, and E. W. Meijer, Colloid Polym. Sci. 280, 416 (2002).

    Article  CAS  Google Scholar 

  28. 28.

    A. V. Lezov, A. B. Mel’nikov, G. E. Polushina, et al., Dokl. Akad. Nauk 381, 69 (2001).

    CAS  Google Scholar 

  29. 29.

    E. A. Tatarinova, E. A. Rebrov, V. D. Myakushev, et al., Izv. Akad. Nauk, Ser. Khim., No. 11, 2484 (2004).

  30. 30.

    H. Mori, D. C. Seng, H. Lecher, et al., Macromolecules 35, 9270 (2002).

    Article  CAS  Google Scholar 

  31. 31.

    H. Mori, A. Walther, X. Andre, et al., Macromolecules 37, 2054 (2004).

    Article  CAS  Google Scholar 

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Correspondence to A. P. Filippov.

Additional information

Original Russian Text © A.A. Shpyrkov, I.I. Tarasenko, G.A. Pankova, I.E. Il’ina, E.V. Tarasova, E.B. Tarabukina, G.P. Vlasov, A.P. Filippov, 2009, published in Vysokomolekulyarnye Soedineniya, Ser. A, 2009, Vol. 51, No. 3, pp. 377–386.

This work was supported by the Russian Foundation for Basic Research (project nos. 05-03-33152 and 07-03-00290) and the program of Basic Research of the Division of Chemistry and Materials Sciences, Russian Academy of Sciences “Creation and Study of Macromolecules and Macromolecular Structures of New Generations.”

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Shpyrkov, A.A., Tarasenko, I.I., Pankova, G.A. et al. Molecular mass characteristics and hydrodynamic and conformational properties of hyperbranched poly-L-lysines. Polym. Sci. Ser. A 51, 250–258 (2009).

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  • Polymer Science Series
  • Intrinsic Viscosity
  • Hydrodynamic Radius
  • Hyperbranched Polymer
  • Static Light Scattering