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Biochemical oxidation of high-viscosity oil by indigenous soil microflora

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Abstract

The influence of high-viscosity crude oil from the Usa field on the oxygenase activity of indigenous soil microflora has been investigated in the laboratory. After an adaptation period, microorganisms have adapted to hydrocarbons of high-viscosity oil and the rate of biochemical oxidation has increased. Oil utilization reached 50% within 60 days of the experiment. IR analysis of residual petroleum hydrocarbons showed the appearance of absorption bands at 1070, 1165, 1730, 1270, and 3300 cm−1, which indicate the presence of a large amount of oxygenated compounds and, hence, the occurrence of petroleum hydrocarbon oxidation processes. Gas chromatographic-mass spectrometric analysis has confirmed a high biodegradation rate: utilization of acyclic saturated hydrocarbons has made 77.5%; cyclic hydrocarbons, 99.2%, alkylbenzenes, 99%; and biaromatic, triaromatic, and polyaromatic hydrocarbons, 97–99, 96, and 97%, respectively.

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Correspondence to D. A. Filatov.

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Original Russian Text © D.A. Filatov, E.V. Gulaya, L.I. Svarovskaya, L.K. Altunina, 2013, published in Neftekhimiya, 2013, Vol. 53, No. 1, pp. 64–69.

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Filatov, D.A., Gulaya, E.V., Svarovskaya, L.I. et al. Biochemical oxidation of high-viscosity oil by indigenous soil microflora. Pet. Chem. 53, 59–64 (2013). https://doi.org/10.1134/S0965544113010040

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