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Experimental investigation of vortex structure formation in a gas-vortex bioreactor

Thermophysics and Aeromechanics volume 29, pages 683–688 (2022)Cite this article

Abstract

The formation of circulation vortex cells in a liquid medium of a gas-vortex bioreactor has been experimentally studied. The study was carried out in an industrial glass bioreactor with a volume of 10 liters and a reactor vessel diameter D = 190 mm. The vortex motion of air was generated by a vane wheel (activator) when 50 and 80% of the reactor vessel was filled with the model medium. A 65% water solution of glycerol with density ρ = 1150 kg/m3 and kinematic viscosity ν = 15 mm2/s was used as a model medium. To observe the pattern of vortex motion, the method of particle image velocimetry (PIV) was used. It is shown that when the activator rotates, the meridional and circulation motions of liquid occur simultaneously. Regularities of the vortex motion of the model medium are determined depending on the reactor filling and the intensity of activator rotation. It is found that the cells of centrifugal circulation appear under the interface; with an increase in the activator rotational speed they develop into the depth of the reactor. It is established that centrifugal circulation of liquid develops similarly as it takes place in a circulating vortex flow of one liquid and in a system of confined vortex motion of two immiscible liquids.

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References

  1. A.K. Gupta, D.G. Lilley, and N. Syred, Swirl Flows, Abacus Press, Tunbridge Wells, England, 1984.

    Google Scholar 

  2. A.V. Savelyeva, A.A. Nemudraya, V.F. Podgornyi, N.V. Laburkina, Y.A. Ramazanov, A.P. Repkov, E.V. Kuligina, and V.A. Richter, Analysis of the efficiency of recombinant escherichia coli strain cultivation in a gas-vortex bioreactor, Biotechnology and Applied Biochemistry, 2017, Vol. 64, Iss. 5, P. 712–718.

    Article  Google Scholar 

  3. S. Fang, P.W. Todd, and T.R. Hanley, Enhanced oxygen delivery to a multiphase continuous bioreactor, Chemical Engng Sci., 2017, Vol. 170, P. 597–605.

    Article  Google Scholar 

  4. T.O. Chaplina, Experimental study of substance transfer in vortex and wave flows in multicomponent media, Physical and Mathematical Modeling of Earth and Environment Processes, 2019, P. 159–173.

  5. N.P. Mertvetsov, Yu.A. Ramazanov, A.P. Repkov, A.N. Dudarev, and V.I. Kislykh, Gas-Vortex Bioreactors “BIOK”. Use in Modern Biotechnology, Nauka, Novosibirsk, 2002.

    Google Scholar 

  6. R.G. Gevorgiz, A.A. Gontcharov, S.N. Zheleznova, L.V. Malakhova, T.E. Alyomova, T. Maoka, and M.V. Nekhoroshev, Biotechnological potential of a new strain of cylindrotheca fusiformis producing fatty acids and fucoxanthin, Bioresource Technology Reports, 2022, Vol. 18, P. 101098.

    Article  Google Scholar 

  7. V.N. Shtern, Cellular Flows. Topological Metamorphoses in Fluid Mechanics, Cambridge University Press, Cambridge, 2018.

    Chapter  MATH  Google Scholar 

  8. I.V. Naumov, V.G. Glavniy, B.R. Sharifullin, and V.N. Shtern, Formation of a thin circulation layer in a two-fluid rotating flow, Physical Review Fluids, 2019, Vol. 4, Iss. 5, P. 054702–1–054702–17.

    Article  ADS  Google Scholar 

  9. J.-C. Tsai, C.-Y. Tao, Y.-C. Sun, C.-Y. Lai, K.-H. Huang, W.-T. Juan, and J.-R. Huang, Vortex-induced morphology on a two-fluid interface and the transitions, Phys. Rev. E., 2015, Vol. 92, P. 031002–1–031002–5.

    Article  ADS  Google Scholar 

  10. I.V. Naumov, B. Sharifullin, and V.N. Shtern, Vortex breakdown in the lower fluid of a two-fluid swirling flow, Phys. Fluids, 2020, Vol. 32, No. 1, P. 014101–1–014101–16.

    Article  Google Scholar 

  11. I.V. Naumov, B.R. Sharifullin, M.A. Tsoy, and V.N. Shtern, Dual vortex breakdown in a two-fluid confined flow, Phys. Fluids, 2020, Vol. 32, P. 061706–1–061706–5.

    Article  ADS  Google Scholar 

  12. B.R. Sharifullin and I.V. Naumov, Angular momentum transfer across the interface of two immiscible liquids, Thermophysics and Aeromechanics, 2021, Vol. 28, No. 1, P. 65–76.

    Article  ADS  Google Scholar 

  13. I.V. Naumov, S.G. Skripkin, B.R. Sharifullin, and V.N. Shtern, Study of vortex motion in a gas-liquid bioreactor, Processes in Geomedia, 2021, No. 3(29), P. 1242–1250.

  14. I.V. Naumov, S.G. Skripkin, and V.N. Shtern, Counter flow slip in a two-fluid whirlpool, Phys. Fluids, 2021. Vol. 33, Iss. 6. P. 061705–1–061705–7.

    Article  ADS  Google Scholar 

  15. I.V. Naumov and V.N. Shtern, Two-Story Tornado, Pripoda, 2021, No. 4, P. 12–19.

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Authors and Affiliations

  1. Kutateladze Institute of Thermophysics SB RAS, Novosibirsk, Russia

    I. V. Naumov, R. G. Gevorgiz, S. G. Skripkin & B. R. Sharifullin

  2. Kovalevsky Institute of Biology of the Southern Seas RAS, Sevastopol, Russia

    R. G. Gevorgiz

Authors
  1. I. V. Naumov
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  2. R. G. Gevorgiz
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  3. S. G. Skripkin
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  4. B. R. Sharifullin
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Correspondence to I. V. Naumov.

Additional information

The work was financially supported by a grant of the Russian Science Foundation (project code 19-19-00083).

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Naumov, I.V., Gevorgiz, R.G., Skripkin, S.G. et al. Experimental investigation of vortex structure formation in a gas-vortex bioreactor. Thermophys. Aeromech. 29, 683–688 (2022). https://doi.org/10.1134/S0869864322050067

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  • DOI: https://doi.org/10.1134/S0869864322050067

Keywords

  • hydrodynamics
  • circulating vortex flow
  • heat and mass transfer
  • vortex bioreactors