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Effects of 3-Day Immersion on Blood Proteome in Female Volunteers

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Abstract

Chromatography–mass spectrometry was used to analyze dried blood spots collected from 6 female volunteers in a 3-day dry immersion (DI) test in order to detect proteins involved in acute adaptation of the female physiological systems. Sampling was performed before DI, every day in DI, and on the next day after DI completion. Proteomic analysis demonstrated an immediate reaction of proteins involved in carbohydrate metabolism and proteasome degradation of proteins. On DI day-2, we saw a reaction of immune response proteins and decreased level of PEDF, an angiogenesis inhibitor that controls many processes, including heart protection from injuries. On DI day-3, the response was joined by proteins participating in processes of the complement cascade and coagulation, platelet degranulation, hemostasis, extracellular matrix formation, glycolysis, water–salt balance, antioxidant system, and the neutrophils involving defense response. It should be noted that on DI day-3 we also found increased levels of histidine triad 1 nucleotide-binding protein 1 (HINT1) and myosin light chain 4 (MYL4) that play an important role in cardiac adaptation to a volume overload. Not all of the proteins returned to baseline levels 2 days after DI. Changes in the level of proteins involved in lipid metabolism were observed only after DI completion.

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ACKNOWLEDGMENTS

The team of authors expresses gratitude to Elena Sergeevna Tomilovskaya for the opportunity to participate in the experiment with a 3-day “dry” immersion.

Funding

The work was carried out within the basic theme of the Russian Academy of Sciences 65.3. Work on proteomic analysis of dried blood spot samples was supported by a grant from the Russian Science Foundation No. 22-74-00069.

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Authors and Affiliations

  1. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia

    D. N. Kashirina, L. Kh. Pastushkova, A. G. Brzhozovskii, A. S. Kononikhin & I. M. Larina

  2. Skolkovo Institute of Science and Technology, Moscow, Russia

    A. G. Brzhozovskii, A. S. Kononikhin & E. N. Nikolaev

Authors
  1. D. N. Kashirina
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  2. L. Kh. Pastushkova
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  3. A. G. Brzhozovskii
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  4. A. S. Kononikhin
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  5. E. N. Nikolaev
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  6. I. M. Larina
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Corresponding author

Correspondence to D. N. Kashirina.

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CONFLICT OF INTEREST

The authors of this work declare that they have no conflicts of interest.

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

The study protocol was approved by the Bioethics Commission of the Institute of Medical and Biological Problems of the Russian Academy of Sciences (protocol no. 544 of July 16, 2020) and fully complied with the principles of the 1964 Declaration of Helsinki. Each study participant voluntarily signed an Informed Consent after explaining to her the potential risks, benefits, and nature of the upcoming study.

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Translated by A. Deryabina

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Kashirina, D.N., Pastushkova, L.K., Brzhozovskii, A.G. et al. Effects of 3-Day Immersion on Blood Proteome in Female Volunteers. Hum Physiol 49, 865–874 (2023). https://doi.org/10.1134/S036211972307023X

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  • DOI: https://doi.org/10.1134/S036211972307023X

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