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Maximal Aerobic Effort Increases Genetic Expression of HSP90AA1, HSP90AB1, and PTGES3 in Elite Taekwondo Athletes

Abstract

Heat shock proteins (HSPs) work in response to a variety of external and internal stresses to maintain homeostasis and are upregulated upon cellular damage. This study aimed to determine the effect of maximal aerobic effort test (\({{\dot {V}}}{{{\text{O}}}_{2}}\)max test) on the expression of genes related to heat shock proteins’ (HSPs) cycle (HSP90AA1, HSP90AB1, PTGES3). Eleven black-belt taekwondo male athletes participated in this study and performed a \({{\dot {V}}}{{{\text{O}}}_{2}}\)max test on a treadmill connected to the Cortex gas analyzer till the point of physical exhaustion. Gene expression was analyzed by real-time PCR, while the physiological parameters were measured spectrophotometrically or by immune assays using serum form the blood Before the test (Bf), Immediately After (IA), and Two Hours After (2HA) the \({{\dot {V}}}{{{\text{O}}}_{2}}\)max test. The levels of fold change expression were affected by the VO2max test. HSP90AA1, HSP90AB1 and PTGES3 mRNA levels were significantly higher IA the test (P ≤ 0.05). HSPs surpassed the cut-off value (1.5-fold) IA showed (M = 2.29 times for HSP90AA1), (M = 1.68 times for HSP90AB1), while PTGES3 did not reach the cut-off value (M = 1.12 times). The expression levels of the three genes were significantly decreased 2HA and returned to the baseline values. Moreover, physiological parameters including growth hormone, creatinine kinase, white blood cells, platelets count, and blood lactate showed a peak in their levels IA the test and then dropped 2HA (P ≤ 0.05). Our findings demonstrate that genetic expressions of HSP90AA1 and HSP90AB1 genes were significantly elevated IA the test and decreased 2HA. The same applied on most of the physiological parameters; strongly suggesting the possible utility of using those parameters as a reference to confirm evidence of exercise-induced cell stress.

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ACKNOWLEDGMENTS

Authors would like to express their thankfulness to the athletes who participated in the present study.

Funding

This research was funded by a grant from the University of Jordan (Grant number: 30 049, 2018/2019). The authors report no involvement in the research by the sponsor that could have influenced the outcome of this work.

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Correspondence to B. Azab.

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COMPLIANCE WITH ETHICAL STANDARDS

This study was approved by the Faculty of Graduate Studies in the University of Jordan. Ethical approval was obtained from the Research Ethics Committee (no. 4970 2018−2019). The objective of the study was explained to all participants and a consent form was signed by all the subjects who agreed to participate. All the work was conducted according to the principles of good clinical practice that have their origin in the declaration of Helsinki and good laboratory practice for the analysis of blood samples.

CONFLICTS OF INTERESTS

The authors declare that they have no competing interests with no conflict of interests with any financial organization regarding the material discussed in the manuscript.

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Hammad, R., Srour, L., Hammad, S. et al. Maximal Aerobic Effort Increases Genetic Expression of HSP90AA1, HSP90AB1, and PTGES3 in Elite Taekwondo Athletes. Hum Physiol 48, 254–260 (2022). https://doi.org/10.1134/S0362119722030070

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  • DOI: https://doi.org/10.1134/S0362119722030070

Keywords:

  • HSP90AA1
  • HSP90AB1
  • PTGES3
  • blood
  • VO2max