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Effect of Short-term Dry Immersion on Proteolytic Signaling in the Human Soleus Muscle

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Abstract

We analyzed the signaling processes initiating proteolytic events in the human soleus muscle during short-term exposure under the non-weight-bearing conditions. Dry immersion (DI) was used to induce weight deprivation in the m. soleus for 3 days. Western blotting was used to determine the level of insulin receptor substrate 1 (IRS-1), total and phosphorylated neuronal NO synthase (nNOS), and adenosine monophosphate-activated protein kinase (AMPK), which control the anabolic and catabolic signaling pathways, and the level of cytoskeletal protein desmin and Са2+-activated protease calpain. By day 3 of DI, calpain- dependent proteolysis manifests itself by reductions in both the total content and level of nNOS phosphorilation. The rate of AMPK phosphorylation was significantly decreased.

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Authors and Affiliations

  1. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, 123007, Russia

    N. A. Vil’chinskaya, T. M. Mirzoev, Yu. N. Lomonosova, I. B. Kozlovskaya & B. S. Shenkman

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  1. N. A. Vil’chinskaya
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  2. T. M. Mirzoev
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  3. Yu. N. Lomonosova
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  4. I. B. Kozlovskaya
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  5. B. S. Shenkman
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Correspondence to N. A. Vil’chinskaya.

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Original Russian Text © N.A. Vil’chinskaya, T.M. Mirzoev, Yu.N. Lomonosova, I.B. Kozlovskaya, B.S. Shenkman, 2016, published in Aviakosmicheskaya i Ekologicheskaya Meditsina, 2016, Vol. 50, No. 1, pp. 28–34.

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Vil’chinskaya, N.A., Mirzoev, T.M., Lomonosova, Y.N. et al. Effect of Short-term Dry Immersion on Proteolytic Signaling in the Human Soleus Muscle. Hum Physiol 43, 787–792 (2017). https://doi.org/10.1134/S0362119717070209

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