Abstract
Nine volunteers aged 27 to 42 participated in an experiment with 370-day antiorthostatic hypokinesia at–5°C, and their blood serum samples were tested for the concentrations of lipid peroxidation (LPO) derivatives, including diene conjugates (DCs), malonic dialdehyde (MDA), and Schiff bases (SBs), and indices of the antioxidant defense system, including the tocopherol (TP) concentration and total antioxidant activity (AOA). The subjects were divided into two groups, which differed in physical training regimen and prophylaxis measures. Initial LPO steps were inhibited in both of the groups by 54–73% from day 50, while the level of SBs, which are final LPO products, decreased by 50–61% by day 230 and remained much the same up to the end of the experiment. The MDA and SB concentrations decreased by a factor of 1.6–2.3 during recovery. Total AOA decreased as an aftereffect during recovery to a level far lower than physiologically normal. Based on the significant inhibition of free-radical LPO throughout the experiment, long-term adaptation to simulated hypogravity was accompanied by a pronounced decrease in biological oxidation and caused severe stress. Substantial long-term readaptation stress developed during recovery after 370-day antiorthostatic hypokinesia, as was evident from the facts that the LPO activity was almost halved, TP concentration significantly increased, and the functional reserves of water-soluble antioxidants were exhausted. Lack of LPO activation was assumed to reflect adequate compensation in the subjects.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Markin, A.A., Zhuravleva, O.A., Morukov, B.V., et al., Changes in metabolic processes in humans during a 120-day antiorthostatic hypokinesia, Hum. Phys., 2009, vol. 35, no. 4, p. 449.
Gunina, L.A., Oxidative stress and adaptation: Metabolic aspect in the effect of exercise, Nauka Olimp. Sporte, 2013, no. 4, p. 19.
Baraboi, V.A., Bioantioksidanty (Biological Antioxidants), Kiev: Kniga plyus, 2006.
Huerta, J.M., Gonzalez, S., Fernandez, S., et al., Lipid peroxidation, antioxidant status and survival in institutionalized elderly: A five-year longitudinal study, J. Free Radical Res., 2006, vol. 40, no. 6, p. 571.
Vinogradov, V.V., Stress i patologiya (Stress and Pathology), Minsk: Belorusskaya nauka, 2007.
Surina-Marysheva, E.F., Lipid peroxidation intensity in immobilization stress, Vestn. YuUrGU, 2008, no. 4, p. 86.
Fatouros, I.G., Jamurtas, A.Z., Villiotou, V., et al., Oxidative stress responses in older men during endurance training and detraining, J. Med. Sci. Sports Exerc., 2004, vol. 36, no. 12, p. 2065.
Vladimirov, Yu.A. and Archakov, A.I., Perekisnoe okislenie lipidov v biologicheskikh membranakh (Lipid Peroxidation in Biological Membranes), Moscow: Nauka, 1972.
Davydov, B.V. and Golikov, P.P., Lipid peroxidation and the a-tocopherol level in the liver in rats with developing myocardial infarction, Serdechnaya nedostatochnost' v ostrom periode infarkta miokarda (Heart Failure in the Acute Phase of Myocardial Infarction), Moscow, 1987, pp. 125–131.
Delenyan, N.V. and Markin, A.A., State of the lipid peroxidation system in rat tissues after a 7-day space flight onboard the Kosmos 1667 biological orbiter, Kosm. Biol. Aviakosm. Med., 1988, vol. 23, no. 4, p. 34.
Yagi, K., A simple fluorometric assay for lipoperoxide in blood plasma, Biochem. Med, 1976, vol. 15, no. 2, p. 212.
Stocks, J., Gutteridge, J.M.C., Sharp, R.J., et al., Assay using brain homogenate for measuring the antioxidant activity of biological fluids, Clin. Sci. Mol. Med., 1974, vol. 47, no. 3, p. 215.
Zaks, L., Statisticheskoe otsenivanie (Statistical Evaluation), Moscow, 1976.
Belyakov, N.A. and Semes’ko, S.G., Antioxidant activity of biological fluids in humans: Methods and clinical significance, Efferentn. Ter., 2005, vol. 11, no. 1, p. 5.
Kamyshnikov, V.S., Spravochnik po klinikobiokhimicheskim issledovaniyam i laboratornoi diagnostike (Guidebook on Clinical Chemistry Tests and Laboratory Diagnosis), Moscow: MEDpress-inform, 2009.
Naidina, V.P., Larina, I.M., Sukhanov, Yu.V., and Zharkovskaya, E.E., Phospholipid compositions of membranes and the blood serum and the serum content of prostaglandins during long-term antiorthostatic hypokinesia, Kosm. Biol. Aviakosm. Med., 1991, vol. 25, no. 5, p. 33.
Mikhailov, V.M., Popova, I.A., Larina, I.M., and Buravkova, L.B., Hormonal mechanisms providing for muscular activity under conditions of prolonged antiorthostatic hypokinesia, Hum. Phys., 1999, vol. 25, no. 3, p. 351.
Klenova, N.A., Biokhimiya patologicheskikh sostoyanii: uchebnoe posobie (Biochemistry of Pathological Conditions: A Handbook), Klenova, N.A., Ed., Samara: Izdvo “Samarskii universitet”, 2006.
Kamskova, Yu.G., Changes in antioxidant status and lipid peroxidation level in the course of 30-day hypokinesia, Byull. Eksp. Biol. Med., 2001, no. 10, p. 387.
Markin, A.A. and Zhuravleva, O.A., Blood chemistry testing, in Orbital’naya stantsiya “Mir” (Mir Space Station), Moscow, 2001, vol. 1, chapt. 14, p. 606.
Zhuravleva, O.A., Morukov, B.V., Markin, A.A., et al., Lipid peroxidation rate and the antioxidant protection system during the readaptation period after long-term space flights on board the international space station, Hum. Phys., 2011, vol. 37, no. 3, p. 92.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.A. Juravlyova, A.A. Markin, D.S. Kuzichkin, V.I. Loginov, I.V. Zabolotskaya, L.V. Vostrikova, 2016, published in Fiziologiya Cheloveka, 2016, Vol. 42, No. 1, pp. 94–99.
Rights and permissions
About this article
Cite this article
Juravlyova, O.A., Markin, A.A., Kuzichkin, D.S. et al. Dynamics of oxidation stress markers during long-term antiorthostatic hypokinesia: A retrospective study. Hum Physiol 42, 79–83 (2016). https://doi.org/10.1134/S0362119715060109
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0362119715060109