Skip to main content
SpringerLink
Log in

Preservation of leukocytes at moderately low temperatures

  • Methods
  • Published:
Human Physiology Aims and scope Submit manuscript

Abstract

It was shown that the use of low-toxic cryopreservative based on glycerol (endocellular protectant), hydroxyethyl starch (exocellular protectant), and 3-hydroxy-6-methyl-2-ethylpyridine succinate (restoring component) makes it possible to preserve nucleated blood cells in a viable state at −40°C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kornienko, E.M. and Bondarenko, V.A., The Promise of Using Combined Cryoprotectants When Freezing Blood Components by the One-Step Method at a Temperature of −196°C, Probl. Kriobiol., 2008, vol. 18, no. 2, p. 248.

    Google Scholar 

  2. Ramazanov, V.V., The Influence of Combined Media on the Damage of Red Blood Cells Frozen with Different Hematocrit, Probl. Kriobiol., 2006, vol. 16, no. 2, p. 155.

    CAS  Google Scholar 

  3. Belous, A.M. and Grishchenko, V.I., Kriobiologiya (Cryobiology), Kiev: Naukova dumka, 1994.

    Google Scholar 

  4. Belous, A.M. and Bondarenko, V.A., Strukturnye izmeneniya biologicheskikh membran pri okhlazhdenii (Structural Changes in Biological Membranes during Cooling), Kiev: Naukova dumka, 1982.

    Google Scholar 

  5. Zakon Rossiiskoi Federatsii o donorstve krovi i ee komponentov 1 5142-1 (Law of the Russian Federation on the Donation of Blood and Blood Components no. 5142-1) Issued 09.06.1993, Moscow: Dom Sovetov Rossii, 1993.

  6. Svedentsov, E.P., Tumanova, T.V., Khudyakov, A.N., et al., Cryopreservation of Functionally Active Blood Nuclear Cell Membranes at −80°C, Biochemistry (Moscow), Ser. A: Membr. Cell Biol., 2008, vol. 25, no. 1, p. 19.

    Google Scholar 

  7. Slavinskii, A.A. and Nikitina, G.V., Cytochemical Detection of Cationic Proteins in Blood Granulocytes with Amide Black 10B for Visual Evaluation and Computer Analysis of Images, Klin. Lab. Diagn., 1999, no. 2, p. 35.

  8. Glantz, S., Mediko-biologicheskaya statistika (Medical and Biological Statistics), Moscow: Praktika, 1998.

    Google Scholar 

  9. Pushkar’, N.S., Lobyntseva, G.S., Polyakova, A.I., et al., Development of Optimum Conditions of Programmed Freezing of Leukocytes, Probl. Gematol. Pereliv. Krovi, 1978, no. 8, p. 8.

  10. Gol’dina, O.A. and Gorbachevskii, Yu.V., Advantage of Modern Hydroxyethyl Starch Products in the Series of Plasma-Infusion Solutions, Vestnik Sluzhby Krovi Rossii, 1998, no. 3, p. 41.

  11. Devyatkina, T.A., Lutsenko, R.V., Vazhnichaya, E.M., and Smirnov, L.D., Effect of Mexidol and Its Structural Components on the Carbohydrate Content and Lipid Peroxidation in Acute Stress, Vopr. Med. Khim., 1999, vol. 45, no. 3, p. 246.

    CAS  Google Scholar 

  12. Belous, A.M., Gordienko, E.A., and Rozanov, L.F., Biokhimiya membrane (Biochemistry of Membranes), Book 3: Zamorazhivanie i krioprotektsiya (Freezing and Cryoprotection), Moscow: Vysshaya shkola, 1987.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Physiology, Komi Research Center, Urals Branch, Russian Academy of Sciences, Syktyvkar, Russia

    E. P. Svedentsov, D. S. Laptev, T. V. Polezhaeva, O. O. Zaitseva, A. N. Khudyakov & O. N. Solomina

Authors
  1. E. P. Svedentsov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. S. Laptev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. V. Polezhaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. O. O. Zaitseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. N. Khudyakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. O. N. Solomina
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Original Russian Text © E.P. Svedentsov, D.S. Laptev, T.V. Polezhaeva, O.O. Zaitseva, A.N. Khudyakov, O.N. Solomina, 2012, published in Fiziologiya Cheloveka, 2012, Vol. 38, No. 5, pp. 124–128.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Svedentsov, E.P., Laptev, D.S., Polezhaeva, T.V. et al. Preservation of leukocytes at moderately low temperatures. Hum Physiol 38, 558–561 (2012). https://doi.org/10.1134/S036211971205012X

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S036211971205012X

Keywords

Search

Navigation