Abstract
The tryptophan fluorescence of bovine serum albumin (BSA) in solutions with different concentrations of cationic detergent cetyltrimethylammonium bromide (CTAB) at different pH is investigated, providing information on BSA denaturation under the action of CTAB. It is found that BSA denaturation under the action of CTAB at all of the investigated pH values (3.5–8.0) is a single-stage process, as determined by BSA tryptophan fluorescence quenching, by an increased degree of the BSA tryptophan fluorescence polarization, and by the values of the parameters for the rotational diffusion of BSA molecules in CTAB solutions. It is shown that the cationic detergent CTAB is more efficient for BSA denaturation at pH values higher than the BSA isoelectric point (4.9).
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References
Yu. A. Gryzunov and G. E. Dobretsov, Blood Serum Albumin in Clinical Medicine (IRIUS, Moscow, 1994) [in Russian].
V. M. Stepanov, Molecular Biology. Structure and Functions of Proteins (Mosc. Gos. Univ., Moscow, 2005) [in Russian].
L. A. Osterman, Methods of Protein and Nucleic Acid Research (MTsNMO, Moscow, 2002) [in Russian].
R. Dawson, D. Elliott, W. Elliott, and K. Jones, Data for Biochemical Research (Clarendon, Oxford, 1986).
R. B. Gennis, Biomembranes: Molecular Structure and Function (Springer, New York, 1989; Mir, Moscow, 1997).
I. M. Vlasova, D. V. Polyansky, and A. M. Saletsky, Laser Phys. Lett. 4, 890 (2007).
I. M. Vlasova and A. M. Saletsky, J. Appl. Spectrosc. 76, 536 (2009).
I. M. Vlasova and A. M. Saletsky, Vestn Mosk. Univ., Ser. Fiz. Astron., No. 1, 58 (2010).
I. M. Vlasova, A. Yu. Zemlyanskii, and A. M. Saletsky, J. Appl. Spectrosc. 73, 743 (2006).
I. M. Vlasova, A. A. Vlasov, and A. M. Saletsky, J. Mol. Struct. 984, 332 (2010).
I. M. Vlasova, V. V. Zhuravleva, and A. M. Saletsky, Russ. J. Phys. Chem. A 86, 509 (2012)
M. Dockal, D. C. Carter, and F. Ruker, J. Biol. Chem. 275, 3042 (2000).
E. L. Gelamo, C. H. T. P. Silva, H. Imasato, and M. Tabak, Biochem. Biophys. Acta 1594, 84 (2002).
E. L. Gelamo and M. Tabak, Spectrochim. Acta A 56, 2255 (2000).
E. Lissi, E. Abuin, M. E. Lanio, and C. Alvarez, J. Biochem. Biophys. Methods 50, 261 (2002).
Y. Moriyama, D. Ohta, K. Hachiya, Y. Mitsui, and K. Takeda, J. Protein Chem. 15, 265 (1996).
L. V. Levshin and A. M. Saletskii, Optical Methods of Investigation of Molecular Systems. I. Molecular Spectroscopy (Mosk. Gos. Univ., Moscow, 1994) [in Russian].
V. Schmidt, Optical Spectroscopy in Chemistry and Life Sciences (Wiley-VCH, Weinheim, 2005; Tekhnosfera, Moscow, 2007).
I. Tinoko, Jr., K. Sauer, J. Wang, and J. Puglisi, Physical Chemistry. Principles and Application in Biological Sciences (Prentice Hall, New Jersey, 1995; Tekhnosfera, Moscow, 2005).
I. M. Vlasova and A. M. Saletsky, Russ. J. Phys. Chem. A 85, 876 (2011).
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Original Russian Text © I.M. Vlasova, V.V. Zhuravleva, A.M. Saletskii, 2013, published in Zhurnal Fizicheskoi Khimii, 2013, Vol. 87, No. 6, pp. 1040–1047.
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Vlasova, I.M., Zhuravleva, V.V. & Saletskii, A.M. Denaturation of bovine serum albumin under the action of cetyltrimethylammonium bromide, according to data from fluorescence analysis. Russ. J. Phys. Chem. 87, 1027–1034 (2013). https://doi.org/10.1134/S0036024413060319
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DOI: https://doi.org/10.1134/S0036024413060319